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Ez pcr kit

Manufactured by Geneflow
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The EZ-PCR kit is a laboratory product designed to facilitate the polymerase chain reaction (PCR) process. It contains the necessary reagents and components required for the amplification of DNA sequences.

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Lab products found in correlation

Rpmi 1640, by Thermo Fisher Scientific (4 mentions) Dulbecco modified eagle medium (dmem), by Merck Group (2 mentions) Transit lt1, by Mirus Bio (2 mentions) Block it lentiviral rnai expression system, by Thermo Fisher Scientific (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Bovine pituitary extract, by Thermo Fisher Scientific (1 mentions) Epidermal growth factor (egf), by Thermo Fisher Scientific (1 mentions) Lncap, by American Type Culture Collection (1 mentions) Foetal calf serum, by Thermo Fisher Scientific (1 mentions) Lncap, by Thermo Fisher Scientific (1 mentions) Keratinocyte serum free medium k sfm, by Thermo Fisher Scientific (1 mentions) Pnt1a, by American Type Culture Collection (1 mentions) L glutamine, by Lonza (1 mentions) Ham s f 12 medium, by Lonza (1 mentions)

5 protocols using ez pcr kit

1

HNSCC Cell Lines and Knockdown

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HNSCC (92-VU-040T, 93-VU-147T) cell lines were kindly provided by Prof Hans Joenje (VU University Medical Center, Amsterdam, the Netherlands), and HeLa cells were obtained from ECACC. All cells were maintained in DMEM (Sigma-Aldrich) supplemented with 10% fetal bovine serum, penicillin (105 U/l), and streptomycin (100 mg/l). Cells were cultured at low passage and authenticated by short tandem repeat analysis (NorthGene, Newcastle upon Tyne, UK) (Supplementary Fig. S1A and S1B) and tested for mycoplasma contamination (EZ-PCR kit; Geneflow). HNSCC cells stably transduced with PTTG, PBF or Scrambled (Scr) shRNA were generated using the BLOCK-iT lentiviral RNAi expression system (Invitrogen) or pre-designed, transduction-ready SMARTvector 2.0 lentiviral particles expressing specific shRNA sequences (Dharmacon) as per manufacturer’s instructions. Further information on HNSCC cells and shRNA sequences are provided (Supplementary Tables S3 and S4).
Read M.L., Modasia B., Fletcher A., Thompson R.J., Brookes K., Rae P.C., Nieto H.R., Poole V.L., Roberts S., Campbell M.J., Boelaert K., Turnell A.S., Smith V.E., Mehanna H, & McCabe C.J. (2018). PTTG and PBF functionally interact with p53 and predict overall survival in head and neck cancer. Cancer research, 78(20), 5863-5876.
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2

Cell Culture Conditions of Prostate Lines

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PNT1A, PC3 and LNCaP cells were purchased from American Type Culture Collection (ATCC). P4E6 were derived in our laboratory51 (link) and BPH1 were a kind gift from Dr Hayward (NorthShore University HealthSystem Research Institute). PNT1A and LNCaP were cultured in Roswell Park Memorial Institute medium (RPMI-1640) (Invitrogen Ltd) supplemented with 10% Foetal Calf Serum (FCS), and 2 mM L-Glutamine (Invitrogen Ltd). BPH1 cells were cultured in RPMI-1640 supplemented with 5% FCS, and 2 mM L-Glutamine. P4E6 cells were cultured in Keratinocyte Serum-Free Medium (KSFM) (Invitrogen Ltd) supplemented with 2% FCS, 2 mM L-Glutamine, 5 ng/ml Epidermal Growth Factor (EGF), 50 μg/ml bovine pituitary extract (Invitrogen Ltd). PC3 cells were cultured in Ham’s F-12 medium (Lonza) supplemented with 7% FCS and 2 mM L-Glutamine. All cell lines were cultured at low passage and routinely tested for Mycoplasma contamination (EZ-PCR kit; Geneflow).
Seed R.I., Taurozzi A.J., Wilcock D.J., Nappo G., Erb H.H., Read M.L., Gurney M., Archer L.K., Ito S., Rumsby M.G., Petrie J.L., Clayton A., Maitland N.J, & Collins A.T. (2019). The putative tumour suppressor protein Latexin is secreted by prostate luminal cells and is downregulated in malignancy. Scientific Reports, 9, 5120.
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3

Thyroid Cell Culture and Transfection

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Human thyroid samples were obtained with local ethics committee approval (Birmingham Clinical Research Office, UK) and informed patient consent. Thyroid TPC-1 cells were kindly provided by Dr Rebecca Schweppe (University of Colorado, Denver, CO, USA) and maintained in RPMI 1640 (Life Technologies, Paisley, UK) supplemented with 10% fetal bovine serum, penicillin (105 U/l), and streptomycin (100 mg/l). Cells were cultured as recommended at low passage and authenticated by short tandem repeat analysis (DNA Diagnostics Centre, London, UK) and tested for mycoplasma contamination (EZ-PCR kit; Geneflow, Lichfield, UK). pEFP BRAFV600E containing BRAFV600E cDNA was kindly provided by Jim Fagin (Memorial Sloan-Kettering Cancer Center, New York, USA). Cells were transfected using TransIT LT1 (Mirus Bio LLC, Madison, WI, USA) according to manufacturer's protocol.
Read M.L., Fong J.C., Modasia B., Fletcher A., Imruetaicharoenchoke W., Thompson R., Nieto H., Reynolds J.J., Bacon A., Mallick U., Hackshaw A., Watkinson J.C., Boelaert K., Turnell A.S., Smith V.E, & McCabe C.J. (2017). Elevated PTTG and PBF predicts poor patient outcome and modulates DNA damage response genes in thyroid cancer. Oncogene, 36(37), 5296-5308.
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4

Thyroid Cell Culture and Transfection

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Human thyroid samples were obtained with local ethics committee approval (Birmingham Clinical Research Office, UK) and informed patient consent. Thyroid TPC-1 cells were kindly provided by Dr Rebecca Schweppe (University of Colorado, Denver, CO, USA) and maintained in RPMI 1640 (Life Technologies, Paisley, UK) supplemented with 10% fetal bovine serum, penicillin (105 U/l), and streptomycin (100 mg/l). Cells were cultured as recommended at low passage and authenticated by short tandem repeat analysis (DNA Diagnostics Centre, London, UK) and tested for mycoplasma contamination (EZ-PCR kit; Geneflow, Lichfield, UK). pEFP BRAFV600E containing BRAFV600E cDNA was kindly provided by Jim Fagin (Memorial Sloan-Kettering Cancer Center, New York, USA). Cells were transfected using TransIT LT1 (Mirus Bio LLC, Madison, WI, USA) according to manufacturer's protocol.
Read M.L., Fong J.C., Modasia B., Fletcher A., Imruetaicharoenchoke W., Thompson R., Nieto H., Reynolds J.J., Bacon A., Mallick U., Hackshaw A., Watkinson J.C., Boelaert K., Turnell A.S., Smith V.E, & McCabe C.J. (2017). Elevated PTTG and PBF predicts poor patient outcome and modulates DNA damage response genes in thyroid cancer. Oncogene, 36(37), 5296-5308.
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5

Thyroid and Cervical Cancer Cell Culture

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Thyroid (TPC-1, 8505C, SW1736) cancer cell lines were maintained in RPMI-1640 (Life Technologies), while HeLa and HEK293 cancer cells were maintained in DMEM (Sigma-Aldrich). Media was supplemented with 10% fetal bovine serum (FBS), penicillin (105 U/l), and streptomycin (100 mg/l) and cell lines were maintained at 37ºC and 5% CO2 in a humidified environment. Cell lines were obtained from ECACC (HEK293, HeLa) and DSMZ (8505C), while TPC-1 and SW1736 cell lines were kindly provided by Dr Rebecca Schweppe (University of Colorado). Cells were cultured at low passage, authenticated by short tandem repeat analysis (NorthGene; Supp Fig. S1) and tested for mycoplasma contamination (EZ-PCR kit; Geneflow; latest test - 7/2023). Thawed cells were cultured for at least 2 weeks prior to use. Stable TPC-1-NIS and 8505C-NIS cells were generated by transfection of parental TPC-1 or 8505C cells with pcDNA3.1-NIS. Geneticin-resistant monoclonal colonies were expanded following FACS single cell sorting (University of Birmingham Flow Cytometry Facility), and Western blotting used to confirm NIS expression.
Read M.L., Brookes K., Zha L., Manivannan S., Kim J., Kocbiyik M., Fletcher A., Gorvin C.M., Firth G., Fruhwirth G.O., Nicola J.P., Jhiang S., Ringel M.D., Campbell M.J., Sunassee K., Blower P.J., Boelaert K., Nieto H.R., Smith V.E, & McCabe C.J. (2024). Combined Vorinostat and Chloroquine Inhibit Sodium Iodide Symporter Endocytosis and Enhance Radionuclide Uptake In Vivo. Clinical cancer research : an official journal of the American Association for Cancer Research, 30(7), 1352-1366.
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