Tumor and spleen were harvested and placed in empty 15mL conical tubes. Tissues were cut up with scissors then wrapped with a 5-micron Nylon filter paper (Sterlitech) and stuffed filter down into a 1.5 mL conical tube making sure the tissue did not touch the bottom. Tissues were centrifuged at 4000 rpm for 2 hours. Interstitial fluid was assayed for L-lactate concentration using a colorimetric detection kit according to manufacturer’s protocol (abcam).
Colorimetric detection kit
Manufactured by Abcam
Sourced in Germany
Colorimetric detection kit is a laboratory equipment designed to facilitate quantitative analysis of target analytes through color-based detection. The kit provides the necessary reagents and materials to perform such colorimetric assays, enabling researchers to measure the presence and concentration of specific compounds in their samples.
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Lab products found in correlation
3 protocols using colorimetric detection kit
1
Interstitial Fluid Lactate Quantification
2
Interstitial Fluid Lactate Quantification
3
Cell Proliferation Assay with BrdU
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The cells were seeded into 96-well plates at a concentration of 8000 cells/well, allowed to attach overnight, and incubated with the test substances for 3 days at 37 °C with 5% CO2. Incorporation of BrdU into the DNA was measured with a colorimetric detection kit (Abcam, Berlin, Germany) by following the manufacturer’s instructions. The cells were exposed to the BrdU reagent during the final 24 h of incubation. The cells that were incubated without the BrdU reagent served as the negative control. The absorbance values were read at 450 nm with a spectrophotometric microplate reader (FLUOstar Optima). The mean absorbance for each group was determined after subtracting the mean value of the negative control.
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