Rice spikelets were fixed in PMEG buffer (50 mM PIPES, 10 mM EGTA, 5 mM MgSO4, and 4% glycerol, pH 6.8) containing 4% paraformaldehyde (PFA) for 3 h and washed six times in PMEG buffer for 2 hours. After dehydration using ethanol series, they were embedded in Technovit7100 resin (Heraeus Kulzer), sectioned in 2 μm thick slices using a LM2255 microtome (Leica Microsystems), stained with 0.1% toluidine blue O (Wako Pure Chemicals) and photographed using a BX50 light microscope (Olympus) and a DP50 camera system (Olympus). Cellulosic cell wall staining was conducted according to the method described previously [63 (link)]. Fluorescent images were captured using a Fluoview FV300 CLSM system (Olympus), and pseudo-colored and merged using Photoshop CS4 (Adobe Systems Inc.).
Dp50 camera system
Manufactured by Olympus
Sourced in Japan
The DP50 camera system is a digital microscope camera designed for image capture and documentation. It features a 5.0-megapixel CCD sensor and supports a variety of resolutions for capturing high-quality images. The DP50 connects to a computer via a USB interface, allowing for seamless integration with image analysis software.
Automatically generated - may contain errors
4 protocols using dp50 camera system
1
Microscopic Analysis of Rice Spikelet Anatomy
2
Immunofluorescence Staining of TSHR and nSMase
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After 3 washes with phosphate-buffered saline (PBS), sections were incubated with 2 μg/ml anti-TSHR or anti-nSMase primary antibodies diluted in a 0.5% solution of bovine serum albumin (BSA) in PBS overnight at 4°C. The slides were washed 3 times with PBS and incubated with fluorochrome-conjugated secondary antibodies for 1 hour at room temperature. Then, after 3 washes with PBS, the slides were mounted with glycerol and coverslips. The samples were examined under a fluorescence microscope (OLYMPUS IX 51) equipped with an OLYMPUS DP 50 camera system and analyzed at 40x magnification.
3
Anticancer Effects of Lactobacillus casei
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For each cell line, 5 × 105 cells were seeded in DMEM containing 10% FBS. After 24 hours the cells were washed with PBS and cultured in fresh medium. Cells were treated with distilled water, followed by 100 ng, 1 μg, or 10 μg/ml L. casei extract alone, anti-cancer drugs alone (doxorubicin 10 nM, 5-FU 10 nM, paclitaxel 10 nM, cisplatin 10 nM), or L. casei extract (10 μg/ml) plus anti-cancer drugs (doxorubicin 10 nM, 5-FU 10 nM, paclitaxel 10 nM, cisplatin 10 nM) for 72 hours. The growth rate was determined using an inverted microscope (Olympus Model IX51, Tokyo, Japan) equipped with a DP50 camera system (Olympus, Tokyo, Japan) using the 0.4% trypan blue dye exclusion method. Cell survival was measured as a percentage of the total number of cells (viable plus non-viable). Mean survival was determined by counting four randomly selected non-overlapping fields. Each culture dish represents one determination, with each experiment replicated independently 4–6 times using different cultures.
4
Immunofluorescence Staining Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After deparaffinization, rehydration through a series of xylene and ethanol washes, and 3 washes with phosphate-buffered saline, sections were incubated with 2 μg/mL anti-e-cadherin and anti-TH primary antibodies diluted in a 0.5% solution of bovine serum albumin in PBS overnight at 4°C. The slides were washed 3 times with PBS and incubated for 1 hour at room temperature with fluorescein isothiocyanate (FITC) secondary antibodies. After 3 washes with PBS, the slides were mounted with glycerol and coverslips. The samples were examined under a fluorescence microscope (Zeiss Axiophot) equipped with an Olympus DP 50 camera system.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!