Individual polyphenols were determined by using a reversed-phase HPLC (Waters 2695, Milford, MA, USA), which was equipped with a C18 column (Nova-Pak, 150 × 3.9 mm, 4 μm, Waters), a 600 E multi-solvent delivery system, a Waters U6K sampler and a Waters 2996 photodiode array detector (DAD) [22 (link)]. Briefly, a gradient elution of polyphenols was performed using a binary solvent system (acetonitrile and acidified distilled water (pH 2 with orthophosphoric acid)) under isothermal conditions (25 °C). A detection wavelength range of 210–550 nm was used. The identification of the single compounds was confirmed by comparing their retention times and UV-DAD spectra with those of the corresponding standards, as well as with those reported in the literature [25 (link)]. The amount of each compound (expressed as area unit (AU) × 106), was calculated before and after digestion.
U6k sampler
Manufactured by Waters Corporation
Sourced in United States
The U6K sampler is a laboratory equipment designed for sample collection and handling. It provides automated and precise liquid sampling capabilities for use in various analytical applications.
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Lab products found in correlation
2 protocols using u6k sampler
1
HPLC Analysis of Polyphenol Content
2
Polyphenol Analysis of Prunus spinosa
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The hydroalcoholic extract from branches of Prunus spinosa L. was analysed by HPLC using a Waters (Milford, Massachusetts, USA) 600E multi-solvent delivery system, a
Waters U6K sampler and a Waters 991 photodiode-array detector. The chromatographic conditions were the same as those applied by Pinacho et al. (2015) .
The identity of polyphenols was ascertained using data from HPLC/DAD and by comparison of spectral data with results from Pinacho et al. (2015) .
Waters U6K sampler and a Waters 991 photodiode-array detector. The chromatographic conditions were the same as those applied by Pinacho et al. (2015) .
The identity of polyphenols was ascertained using data from HPLC/DAD and by comparison of spectral data with results from Pinacho et al. (2015) .
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