Enzyme linked immunosorbent assay elisa kit

Insulin levels in plasma and pancreatic tissue homogenates were determined using an Enzyme Linked Immunosorbent Assay (ELISA) kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China). Analysis of glycosylated hemoglobin (HbA1c) content was performed on 100 µl of fresh total blood using a turbidimetric immunoassay performed with a Hitachi 7080 (Tokyo, Japan) automatic biochemistry analyzer. Plasma triglyceride (TG), total cholesterol (TC), and low-density lipoprotein (HDL) levels were measured using the Hitachi 7080 system with the cognate kits.
Kits purchased from Nanjing Jiancheng Bioengineering Institute were used to measure malondialdehyde (MDA) levels, catalase (CAT) activity, superoxide dismutase (SOD) activity, glutathione peroxidase (GSH-Px) activity, and reduced glutathione (GSH) levels in plasma or liver tissue homogenates. An anthrone-sulfuric acid colorimetric method [22] (link) was used to determine liver glycogen content.

In the current study, 24 h after the last CIH exposure, the mice were sacrificed by an overdose of pentobarbital (100 mg/kg i.p.), and the trachea was isolated by blunt dissection. Three successive volumes of 0.3 ml PBS were instilled via the endotracheal tube and aspirated gently. The Bronchoalveolar Lavage Fluid (BALF) was pooled from each aspirate. Each BALF sample was centrifuged at 1,000 × g for 10 min at 4°C, and the supernatants were stored at −80°C until used. The cell pellets were diluted with 0.5 ml PBS. The total cell counts were determined using a hemocytometer by adding 100 μl of the cell suspension to 100 μl 0.4% trypan blue. The differential cell counts were performed using cytocentrifuge preparations (Cytospin 2; Shandon Instruments, Runcorn, United Kingdom) stained with the Wright-Giemsa stain method.
The levels of interleukin (IL)-4, IL-5, and IL-13 in the BALF were determined using an Enzyme-Linked Immunosorbent Assay (ELISA) Kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China), according to the standard protocols.

1-Chloro-2,4-dinitrobenzene (DNCB), acetone, olive oil, and chloral hydrate were obtained from Sinopharm Chemical Reagent Co. (Shanghai, China). Sodium carboxymethylcellulose, absolute ethanol, 4% paraformaldehyde (PFA), xylene, ethylenediaminetetraacetic acid (EDTA), and hematoxylin and eosin were purchased from Wuhan Goodbio Technology Co., Ltd. (Wuhan, China). Prednisone acetate tablets were obtained from Anhui Jintaiyang Pharma (Anhui, China). Enzyme-linked immunosorbent assay (ELISA) kits were purchased from Nanjing Jiancheng Co. (Nanjing, China). Fructus Kochiae was purchased from Anhui Xiehecheng Co., Ltd. (Hehui, China), the place of origin was Henan, and batch code was 15112601. Chromatographically pure acetonitrile was purchased from Tedia (USA). Rutin, batch no. 3920, purity 99.3%, and quercetin, no. 33467, purity 98.6%, were obtained from Shanghai Shidan Biotechnology Co., Ltd. (Shanghai, China).