T cells were quantified by size, granularity and positive expression of CD3 and either CD4 or CD8. T-cell activation was determined using PE-conjugated anti-CD38, FITC-conjugated anti-HLA-DR, peridinin-chlorophyll-protein-complex-conjugated anti-CD3, allophycocyanin-cy7-conjugated anti-CD8 and allophycocyanin-conjugated anti-CD4 antibodies all from BD Biosciences. T-cell activation was defined as expression of co-expression of CD38 and HLA-DR. Measurements were performed using a LSR II flow cytometer and analyzed using FACSDiva software.
Allophycocyanin conjugated anti cd4
Manufactured by BD
Allophycocyanin-conjugated anti-CD4 is a laboratory reagent used for the detection and analysis of CD4-positive cells. It consists of a monoclonal antibody targeting the CD4 receptor, conjugated to the fluorescent dye allophycocyanin. This product can be used in various immunological and cell analysis techniques, such as flow cytometry, to identify and quantify CD4-expressing cells.
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Lab products found in correlation
Peridinin chlorophyll protein complex conjugated anti cd3, by BD (1 mentions)
Fitc conjugated anti hla dr, by BD (1 mentions)
Pe conjugated anti cd38, by BD (1 mentions)
Nf κb p65, by Abcam (1 mentions)
Anti bax, by Cell Signaling Technology (1 mentions)
Concanavalin a (cona), by Merck Group (1 mentions)
Alcian blue stain kit, by Solarbio (1 mentions)
Anti il 1β, by Bioss Antibodies (1 mentions)
Anti il 10, by Bioss Antibodies (1 mentions)
Anti tnf α, by Bioss Antibodies (1 mentions)
Anti caspase 3, by Cell Signaling Technology (1 mentions)
β actin, by Abcam (1 mentions)
Metronidazole, by Solarbio (1 mentions)
Ampicillin, by Solarbio (1 mentions)
Vancomycin, by Solarbio (1 mentions)
Neomycin, by Solarbio (1 mentions)
2 protocols using allophycocyanin conjugated anti cd4
1
Quantifying T-cell Activation Using Flow Cytometry
2
Investigating Gut Inflammation Pathways
Check if the same lab product or an alternative is used in the 5 most similar protocols
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We used the following materials: Con A was acquired from Sigma-Aldrich (St. Louis, MO). BBR, alcian blue stain kit, methylprednisolone (MP), ampicillin, neomycin, metronidazole, and vancomycin were afforded by Solarbio (Beijing, China). Anti-Caspase-3, anti-Bax, and anti-F4/80 antibodies were offered by cell signaling technology (Boston, MA). Anti-TNF-α, anti-interferon-γ, anti-IL-1β, anti-IL-17A, and anti-IL-10 antibodies were purchased from Bioss (Beijing, China). Rabbit anti-mouse primary antibodies, including zonula occludens-1 (ZO-1), occludin, TLR4, NF-κB P65, inhibitor of NF-κB (IκB), phospho-inhibitor of NF-κB (p-IκB), and β-actin, were obtained from Abcam (Cambridge, UK). Allophycocyanin-conjugated anti-CD4, fluorescein isothiocyanate–conjugated anti-IL-17A, and phycoerythrin-conjugated anti-Foxp3 antibodies were purchased from BD (Franklin Lakes, NJ). LPS ELISA kits were supplied by Cloud-clone (Hubei, China).
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