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Rat anti mouse cd45r

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The Rat Anti-mouse CD45R is a laboratory reagent used for the identification and isolation of mouse B cells. It recognizes the CD45R antigen, which is expressed on the surface of mouse B cells. This product can be used in various immunological techniques, such as flow cytometry, to detect and analyze B cell populations.

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Lab products found in correlation

Rabbit anti mouse cd3, by Abcam (2 mentions) Rabbit anti mouse bcl 2, by Abcam (2 mentions) Vector peroxidase substrate kit, by Vector Laboratories (2 mentions) Vectastain abc kit, by Vector Laboratories (2 mentions) Antibody diluent, by Agilent Technologies (2 mentions) Vectashield dapi mounting medium, by Vector Laboratories (2 mentions) Alexa fluor 488 donkey anti goat igg h l, by Thermo Fisher Scientific (2 mentions) Goat polyclonal igg anti mouse cd3ε, by Santa Cruz Biotechnology (2 mentions) Alexa fluor 594 donkey anti rat igg h l, by Thermo Fisher Scientific (2 mentions) Rat anti mouse lamp1, by BD (1 mentions) Rabbit anti mouse α tubulin, by Abcam (1 mentions) Goat anti mouse igg f ab 2, by Jackson ImmunoResearch (1 mentions) Ovalbumin (ova), by Merck Group (1 mentions) Mouse anti β actin, by Abcam (1 mentions) Mouse anti mouse ubiquitin p4d1, by Santa Cruz Biotechnology (1 mentions) Donkey anti rabbit igg alexa 488, by Thermo Fisher Scientific (1 mentions) Goat anti rabbit igg alexa 546, by Thermo Fisher Scientific (1 mentions) Donkey anti rat igg alexa546, by Thermo Fisher Scientific (1 mentions) Donkey anti rat alexa647, by Thermo Fisher Scientific (1 mentions) Mg132, by Merck Group (1 mentions)

5 protocols using rat anti mouse cd45r

1

Immunofluorescence Analysis of Proteasome Components

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We used Rat anti-mouse LAMP1 (BD Bioscience, San Jose, CA), Rabbit anti-mouse α-Tubulin (Abcam), Rabbit anti-mouse γ-Tubulin (Abcam), Rabbit anti-mouse S4/19S RP (Abcam), Rabbit anti-mouse αβ/20S proteasome (Abcam), Rabbit anti-mouse α4/20S proteasome (Abcam), Mouse anti-mouse Ubiquitin P4D1 (Santa Cruz), Rabbit anti-mouse pSyk (Y525/526) (cellsignalling), Rabbit anti-mouse Syk (Abcam), anti-mouse β-actin (Abcam), Rat anti-mouse CD45R (BD Bioscience), Goat anti-mouse IgGFab2 (Jackson ImmunoResearch), Goat anti-mouse IgM Fab2 (Jackson ImmunoResearch). For secondary antibodies: Donkey anti-rabbit IgG-Alexa488 (LifeTech), Goat anti-rabbit IgG-Alexa546 (ThermoScientific), Donkey anti-rat IgG-Alexa546 (ThermoScientific), Donkey anti-rat-Alexa647 (ThermoScientific), Phalloidin-Alexa647 (ThermoScientific), DAPI (Abcam). Ovalbumin was purchased from Sigma-Aldrich, MG-132 and Epoxomicin were purchased from Merk (Millipore).
Ibañez-Vega J., Del Valle Batalla F., Saez J.J., Soza A, & Yuseff M.I. (2019). Proteasome Dependent Actin Remodeling Facilitates Antigen Extraction at the Immune Synapse of B Cells. Frontiers in Immunology, 10, 225.
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2

Immunohistochemistry and Senescence Assays

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Sections from paraffin-embedded tissue were dewaxed and rehydrated following standard protocols. Slides were incubated with primary antibody (Rat Anti-mouse CD45R; BD Pharmingen) (Rabbit Anti-mouse CD3; Abcam)(Rabbit Anti-mouse Bcl-2; Abcam) at a dilution of 1:100 overnight at 4 °C. For detection, the Vectastain ABC Kit was used followed by the Vector Peroxidase Substrate Kit (Vector Labs). Terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick-end labelling (TUNEL) assays were carried out on paraffin-embedded sections according to the manufacturer’s specifications (Calbiochem, Darmstadt, Germany). Senescence associated β-Galactosidase (SAβGal) assay was performed essentially as described [34 (link)].
Riley M.F., You M.J., Multani A.S, & Lozano G. (2015). Mdm2 overexpression and p73 loss exacerbate genomic instability and dampen apoptosis resulting in B-cell lymphoma. Oncogene, 35(3), 358-365.
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3

Immunofluorescence Staining of Salivary Glands

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Salivary glands surgically removed from each mouse at time of euthanasia were placed in 10% phosphate-buffered formalin for 24 h, embedded in paraffin and sectioned at 5 µm thickness. Following deparaffination and dehydration, each section was treated with blocking solution containing donkey serum. Sections were then stained with purified rat anti-mouse CD45R (Clone 30-F11, BD Pharmingen, San Jose, CA, USA) diluted 1:25 and goat polyclonal IgG anti-mouse CD3ε (Clone M-20, Santa Cruz Biotechnology, Santa Cruz, CA, USA) diluted 1:50 in an antibody diluent (Dako, Carpinteria, CA, USA) for 1 h at 25 °C. The slides were then washed with PBS followed by a 1 h incubation with Alexa Fluor 488 donkey anti-goat IgG (H+L) and Alexa Fluor 594 donkey anti-rat IgG (H+L) (Life Technologies, Grand Island, NY, USA). After a thorough wash with PBS, the slides were treated with a Vectashield DAPI-mounting medium (Vector Laboratory, Burlingame, CA, USA) and visualized microscopically at 200×.
Peck A.B., Nguyen C.Q, & Ambrus J. (2021). Early Covert Appearance of Marginal Zone B Cells in Salivary Glands of Sjögren′s Syndrome-Susceptible Mice: Initiators of Subsequent Overt Clinical Disease. International Journal of Molecular Sciences, 22(4), 1919.
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4

Immunofluorescence Staining of Salivary Glands

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Salivary glands surgically removed from each mouse at time of euthanasia were placed in 10% phosphate-buffered formalin for 24 h, embedded in paraffin and sectioned at 5 µm thickness. Following deparaffination and dehydration, each section was treated with blocking solution containing donkey serum. Sections were then stained with purified rat anti-mouse CD45R (Clone 30-F11, BD Pharmingen, San Jose, CA, USA) diluted 1:25 and goat polyclonal IgG anti-mouse CD3ε (Clone M-20, Santa Cruz Biotechnology, Santa Cruz, CA, USA) diluted 1:50 in an antibody diluent (Dako, Carpinteria, CA, USA) for 1 h at 25 °C. The slides were then washed with PBS followed by a 1 h incubation with Alexa Fluor 488 donkey anti-goat IgG (H+L) and Alexa Fluor 594 donkey anti-rat IgG (H+L) (Life Technologies, Grand Island, NY, USA). After a thorough wash with PBS, the slides were treated with a Vectashield DAPI-mounting medium (Vector Laboratory, Burlingame, CA, USA) and visualized microscopically at 200×.
Peck A.B., Nguyen C.Q, & Ambrus J.L. (2021). Upregulated Chemokine and Rho-GTPase Genes Define Immune Cell Emigration into Salivary Glands of Sjögren’s Syndrome-Susceptible C57BL/6.NOD-Aec1Aec2 Mice. International Journal of Molecular Sciences, 22(13), 7176.
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5

Immunohistochemistry and Senescence Assays

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Sections from paraffin-embedded tissue were dewaxed and rehydrated following standard protocols. Slides were incubated with primary antibody (Rat Anti-mouse CD45R; BD Pharmingen) (Rabbit Anti-mouse CD3; Abcam)(Rabbit Anti-mouse Bcl-2; Abcam) at a dilution of 1:100 overnight at 4 °C. For detection, the Vectastain ABC Kit was used followed by the Vector Peroxidase Substrate Kit (Vector Labs). Terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick-end labelling (TUNEL) assays were carried out on paraffin-embedded sections according to the manufacturer’s specifications (Calbiochem, Darmstadt, Germany). Senescence associated β-Galactosidase (SAβGal) assay was performed essentially as described [34 (link)].
Riley M.F., You M.J., Multani A.S, & Lozano G. (2015). Mdm2 overexpression and p73 loss exacerbate genomic instability and dampen apoptosis resulting in B-cell lymphoma. Oncogene, 35(3), 358-365.
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