Osteosarcoma cells Saos-2 (CL-0202), SW1353 (CL-0447), and HOS (iCell-h099) were purchased from Procell Company (Wuhan, China). U-2OS (ZQ0121), MG63 (ZQ0403), and 143B (ZQ0455) were purchased from Zhong Qiao Xin Zhou Biotechnology Company (Shanghai, China). Osteoblast hFOB 1.19 (ZQ0402) was purchased from Zhong Qiao Xin Zhou Biotechnology Company. All of the cell lines were authenticated by short tandem repeat DNA profiling and were found to be free of mycoplasma infection. Saos-2 cells were cultured in McCoy’s 5A medium (PM150710, Procell Company) with 15% fetal bovine serum (FBS) (P10033, Hyclone, USA). SW1353, U-2OS, and 143B cells were cultured in Dulbecco’s modified Eagle medium (DMEM) (12100-46, Gibco, USA) with 10% FBS. MG63 and HOS cells were cultured in minimum Eagle’s medium with 10% FBS, and hFOB 1.19 cells were cultured in DMEM/F12 medium with 10% FBS and 0.3 mg/ml G418. All cells were maintained in the humidified indicators at 37 °C with 5% CO2.
Mccoy s 5a medium
Manufactured by Procell
Sourced in China, United States
McCoy's 5A medium is a cell culture medium formulation designed for the growth and maintenance of various cell types. It provides essential nutrients, growth factors, and a buffered environment to support cell proliferation and viability. The specific composition and properties of McCoy's 5A medium are designed to cater to the needs of a wide range of cell lines, making it a versatile choice for cell culture applications.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (12 mentions)
Hct116, by Procell (6 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (6 mentions)
Rpmi 1640 medium, by Procell (4 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (4 mentions)
Skov3, by Procell (3 mentions)
Ovcar3, by Procell (3 mentions)
A2780, by Procell (3 mentions)
Caki 1, by Procell (3 mentions)
Ethanol, by Xilong (3 mentions)
Petroleum ether, by Xilong (3 mentions)
Ethyl acetate, by Xilong (3 mentions)
N butanol, by Xilong (3 mentions)
A2780, by Thermo Fisher Scientific (2 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions)
Minimum essential medium (mem), by Procell (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Chamq universal sybr qpcr master mix, by Vazyme (2 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)
Caov 3, by Procell (2 mentions)
31 protocols using mccoy s 5a medium
1
Osteosarcoma Cell Lines Characterization
2
ACSM3 Regulation in Ovarian Cancer Cells
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OV-90, SK-OV-3, OVCAR-3, and A2780 cells were purchased from Procell Life Science&Technology Co., Ltd (Wuhan, China). OV-90 and OVCAR-3 cells were maintained in a specific medium (Procell). SK-OV-3 cells were cultured in McCoy’s 5A medium (Procell). A2780 cells were cultured in DEME (Gibco Life Technologies, NY, USA). All the medium was supplemented with 10% fetal bovine serum (FBS). And cells were incubated in 5% CO2 at 37°C.
Lentiviral vectors expressing small hairpin RNA (shRNA) targeting ACSM3 were named Lv-shRNA1-ACSM3 or Lv-shRNA2-ACSM3. The complementary cDNAs of ACSM3 were synthesized and the lentiviral overexpressed vectors pcDNA3.1 (GenScript, Nanjing, China) of ACSM3 were constructed as Lv-ACSM3. The shRNA sequences were listed as follow. shRNA1-ACSM3: CGATGTTAAGATTGTAGATGT. shRNA2-ACSM3: GCTTGTACAGAATGATATAAC. The Lv-shRNA1-ACSM3 or the Lv-shRNA2-ACSM3 were infected into OV-90 cells. The Lv-ACSM3 was infected into A2780 or SK-OV-3 cells. The overexpressed ITG β1 (Integrin β1) vectors were transfected into A2780 cells by Lipofectamine 3000 (Invitrogen, Carlsbad, California, USA) according to the manufacturer’s instructions.
Lentiviral vectors expressing small hairpin RNA (shRNA) targeting ACSM3 were named Lv-shRNA1-ACSM3 or Lv-shRNA2-ACSM3. The complementary cDNAs of ACSM3 were synthesized and the lentiviral overexpressed vectors pcDNA3.1 (GenScript, Nanjing, China) of ACSM3 were constructed as Lv-ACSM3. The shRNA sequences were listed as follow. shRNA1-ACSM3: CGATGTTAAGATTGTAGATGT. shRNA2-ACSM3: GCTTGTACAGAATGATATAAC. The Lv-shRNA1-ACSM3 or the Lv-shRNA2-ACSM3 were infected into OV-90 cells. The Lv-ACSM3 was infected into A2780 or SK-OV-3 cells. The overexpressed ITG β1 (Integrin β1) vectors were transfected into A2780 cells by Lipofectamine 3000 (Invitrogen, Carlsbad, California, USA) according to the manufacturer’s instructions.
3
Cell Culture and Transfection Protocol
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BT474, SKBR3, MDA-MB-453, MCF-7, and BT20 cells (purchased from the Shanghai Chinese Academy of Sciences) were cultured routinely. BT474 cells (5 × 105 cells per 2 ml) were grown in RPMI1640, along with 10% fetal bovine serum (FBS) in Nunclon 6-well plates. SKBR3 cells were grown in McCoy's 5A medium (5 × 105 cells per2 ml) (Procell) containing 10% FBS in Nunclon 6-well culture plates. MDA-MB-453, MCF-7, and BT20 cells were cultured in a medium of DMEM (5 × 105 cells per 2 ml) containing 10% FBS in Nunclon 6-well plates. When the fusion degree of cells reached 70% ~ 80%, the cells were transfected. The dosage of transfection reagent and TFO was adjusted according to the requirements of each group. The transfection method was performed according to Lip2000 reagent instructions.
4
Evaluating miR-9-5p in CRC Chemoresistance
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According to previous studies (21 (link),22 (link)), human CRC HCT-116 and HT-29 cell lines were chosen to evaluate the effect of miR-9-5p in CRC chemoresistance. HCT-116 and HT-29 cells were obtained from Procell Life Science & Technology Co., Ltd., and maintained in McCoy's 5A medium (Procell Life Science & Technology Co., Ltd.) containing 10% fetal bovine serum (FBS). 293T cells (Shanghai Zhongqiao Xinzhou Biotechnology Co., Ltd.) were incubated in Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich; Merck KGaA) containing 10% FBS. Cells were cultured at 37°C with 5% CO2.
5
Cell Culture Conditions for Osteosarcoma
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The 293FT cell line was acquired from Thermofisher (catalogue #R700‐07) and maintained in Dulbecco's modified Eagle's medium (DMEM, catalogue #SH30243.01, Gibco) supplemented with 10% fetal bovine serum (FBS) (catalogue #A511‐001, Lonsera). MG‐63 and U2OS osteosarcoma cell lines were procured from Procell and cultured in minimal essential medium (MEM, Procell, China) or McCoy's 5A medium (Procell, China), respectively, with 10% FBS supplementation. All cell lines were preserved under standard conditions of 37°C and 5% CO2. Upon reaching approximately 90% confluence, cells were passaged.
6
Characterization of Renal Cell Carcinoma Lines
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Human renal clear cell carcinoma cell lines 786-O and Caki-1, human normal kidney proximal tubular cell line HK-2, and human monocyte cell line THP-1 were purchased from SIBS (Shanghai, China). Caki-1 cells were cultured with McCoy’s 5A medium (PM150710, Procell, China) and other three cell lines were cultured with RPMI 1640 medium (10-040-CV, CORNING, China), these medium were contained 10% fetal bovine serum (FBS, 10099-141, GIBCO, China) and 1% Penicillin–Streptomycin (E607011, Sangon, China). In addition, THP1 cells were used to induce macrophages by treating with 100 ng/mL phorbol 12-myristate 13-acetate (PMA; Sigma, USA) for 48 h. For further M2 phenotype macrophage induction, cells were treated with 20 ng/mL IL-4 for 48 h in the presence of PMA.
7
Breast Cancer Cell Line Characterization
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The human breast cancer cell lines (T47D, BT549, MCF7, MDA-MB-468, MDA-MB-231, and SKBR3) were purchased from Shaanxi Yike Biotechnology (Xi’an, China). MCF10A cells were cultured in DMEM/F12 (Procell) supplemented with 5% horse serum and 10 μg/mL insulin (Sigma). SKBR3 cells were grown in McCoy’s 5A medium (Procell) containing 10% fetal bovine serum (FBS). T47D and BT549 cells were cultured in a RPMI-1640 medium (Gibco) supplemented with 10% FBS. The MCF7, MDA-MB-231, and MDA-MB-468 cells were grown in DMEM (Gibco) supplemented with 10% FBS. All cells were incubated in a 5% CO2 incubator at 37 °C. Dasatinib (HY-10181) and Adezmapimod (HY-10256) were purchased from MedChemExpress (MCE, Shanghai, China). Phorbol 12-myristate 13-acetate (PMA) was purchased from Solabio (P6741).
8
Culturing Cancer Cell Lines for Research
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HeLa cells, HT29 cells, HCT116 cells, and CT26 cells were obtained from Procell Life Science & Technology Co. Ltd. (Wuhan, China). HeLa cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Procell) with 10% fetal bovine serum (FBS; Gibco) and 1% penicillin-streptomycin (Gibco). HT29 and HCT116 cells were cultured in McCoy’s 5A medium (Procell) with 10% FBS (Gibco) and 1% penicillin-streptomycin (Gibco). CT26 cells were cultured in RPMI 1640 medium (Procell) with 10% FBS (Gibco) and 1% penicillin-streptomycin (Gibco). All the cells were incubated at 37°C with 5% CO2 for culture and passage unless otherwise stated. As for hypoxic condition, HeLa cells were cultured in a hypoxic incubator at 37°C with 1% O2 and 5% CO2.
9
Culturing Human Osteosarcoma Cell Lines
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The human osteosarcoma cell lines MG-63 and Saos-2 were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, United States) and McCoy’s 5A medium (Procell, China) respectively supplemented with 10% fetal bovine serum (FBS) (Gibco). All the cells were cultured in an incubator at 37°C with 5% CO2.
10
Culturing KIRC and HK-2 cell lines
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Both the human KIRC cell line (Caki-1) and the human renal cortex proximal tubule epithelial cells (HK-2) were bought from the American Type Culture Collection. Caki-1 was cultured in McCoy's 5A medium (Procell, Wuhan, China) with 10% fetal bovine serum (Gibco, Thermo Fisher Scientific, United States) and placed in an incubator set at 37°C and 5% CO2. HK-2 was cultured in Minimum Essential Medium (MEM, Procell, Wuhan, China) with 10% fetal bovine serum (Gibco, Thermo Fisher Scientific, United States) and placed in an incubator set at 37°C and 5% CO2.
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