Soluble recombinant human TRAIL was obtained from Enzo Life Sciences (San Diego, CA, USA). The general caspase inhibitor, z-VAD-FMK, and caspase-3/7-specific inhibitor, z-DEVD-FMK, were purchased from Merck Japan (Tokyo, Japan). Glibenclamide, FCCP, antimycin A and U37883A were from Sigma-Aldrich (St. Louis, MO, USA). Potassium chloride (KCl) was obtained from Wako Pure Chemicals (Osaka, Japan). Insoluble reagents (z-VAD-FMK, z-DEVD-FMK, Glibenclamide, FCCP, antimycin A and U37883A) were dissolved in dimethyl sulfoxide (DMSO) and diluted with high glucose-containing Dulbecco’s modified Eagle’s medium (DMEM; Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS; Sigma-Aldrich) or Hank’s balanced salt solution (HBSS) (pH 7.4) to a final concentration of <0.1% prior to use. The manganese-porphyrin superoxide dismutase mimetic MnTBaP (Enzo Life Sciences) were dissolved in 1 mM NaOH (pH 8.0) and added HBSS to lower pH 7.4.
Z devd fmk
Manufactured by Merck Group
Sourced in United States, China, Japan
Z-DEVD-FMK is a lab equipment product manufactured by Merck Group. It is a synthetic tetrapeptide that functions as a potent and irreversible inhibitor of caspase-3, a key enzyme involved in apoptosis (programmed cell death). The core function of Z-DEVD-FMK is to inhibit caspase-3 activity, which can be useful in various research applications related to cell biology and apoptosis.
Automatically generated - may contain errors
Lab products found in correlation
Z vad fmk, by Merck Group (22 mentions)
Z lehd fmk, by Merck Group (11 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (7 mentions)
Puromycin, by Merck Group (4 mentions)
Rabbit anti human β actin antibody, by Merck Group (4 mentions)
Fetal bovine serum (fbs), by Biowest (3 mentions)
Protease inhibitor cocktail, by Merck Group (3 mentions)
P erk, by Cell Signaling Technology (3 mentions)
N acetylcysteine nac, by Merck Group (3 mentions)
Dulbecco s modified eagle s medium dmem, by Merck Group (3 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (3 mentions)
Soluble recombinant human trail, by Enzo Life Sciences (3 mentions)
Sb203580, by Merck Group (3 mentions)
Pd98059, by Merck Group (3 mentions)
Z ietd fmk, by Merck Group (3 mentions)
Trypsin edta, by Thermo Fisher Scientific (3 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (3 mentions)
Salubrinal, by Merck Group (2 mentions)
Dulbecco s modified eagle s medium dmem, by Biowest (2 mentions)
Polyvinylidene difluoride pvdf membrane, by Merck Group (2 mentions)
47 protocols using z devd fmk
1
Caspase Inhibitors and Mitochondrial Modulators
2
Apoptosis Mechanism Investigation Protocol
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Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were obtained from Biowest (Nuaillé, France). Polyvinylidene difluoride (PVDF) membranes and goat anti-rabbit and horseradish peroxidase-conjugated immunoglobulin (Ig) G were obtained from Millipore (Bellerica, MA, USA). MTT, 3-MA, salubrinal, Z-DEVD-FMK, Z-VAD-FMK and β-actin antibody were purchased from Sigma (St. Louis, MO, USA). An annexin V-FITC/PI apoptosis detection kit was purchased from Pharmingen (San Diego, CA, USA). Enhanced chemiluminescence (ECL) reagents were purchased from Pierce Biotechnology (Rockford, IL, USA).
3
Cellular Apoptosis Assays
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WM-3835, all the antibodies, viral constructs, and mRNA primers were provided by Dr. Cao at Soochow University [25 (link)]. The caspase inhibitors zDEVD-fmk and zVAD-fmk, as well as the cell counting kit-8 (CCK-8) kit, docetaxel, and lactate dehydrogenase (LDH) assay kit were purchased from Sigma (St. Louis, MO). All fluorescence dyes were purchased from Thermo-Fisher Invitrogen (Shanghai, China).
4
Apoptosis Induction and Inhibition
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LB-100 was provided by Selleck (Beijing, China). The caspase-3 inhibitor zDEVDfmk and the pan caspase inhibitor zVADfmk were purchased from Sigma (Nanjing, China). All the antibodies of this study were purchased from Cell Signaling Technology (Beverly, MA). The tissue-culture reagents were provide by Gibco (Nanjing, China).
5
Apoptosis Induction and Oxidative Stress Evaluation
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Polybrene, n-acetylcysteine (NAC), ATP, z-DEVD-fmk, z-VAD-fmk, and puromycin were provided by Sigma-Aldrich (St. Louis, MO). IMT1 and antibodies were from Dr. Wang [19 (link)]. Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) antibodies were provided by Dr. Xu [20 (link)]. Fluorescence probes, including TUNEL, tetraethylbenzimidazolylcarbocyanine iodide (JC-1), DAPI (4′,6-diamidino-2-phenylindole), CellROX, dichlorodihydrofluorescein diacetate (DCF-DA), propidium iodide (PI) were obtained from Thermo-Fisher Invitrogen (Shanghai, China).
6
Purification of 13-Acetoxysarcocrassolide from Soft Coral
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13-Acetoxysarcocrassolide was purified after extracting it from the cultured Formosa soft coral S. crassocaule, according to the method described by others (Duh et al. 2000 (link)). Dimethyl sulfoxide (DMSO), 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT), Z-DEVD-FMK (caspase-3 inhibitor), Z-VAD-FMK (a cell-permeable pan-caspase inhibitor), a protease inhibitor cocktail, and rabbit anti-human β-actin antibodies were purchased from Sigma (St. Louis, MO, USA). Antibodies against pro-caspase 9, cleaved-caspase 9, pro-caspase 3, cleaved-caspase 3, cleaved-PARP-1, Bax, Bad, Bcl-xL, Mcl-1, p-Bad, PARP-1, Bcl-2, PI3K, p-PI3K, AKT, p-AKT, mTOR, p-mTOR, p70S6K, p-p70S6K, 4EBP1, p-4EBP1, p-S6, p-eIF4B, and p-eIF4E, and goat anti-rabbit and horseradish peroxidase-conjugated immunoglobulin G were obtained from Cell Signalling Technology (Danvers, MA, USA). Dulbecco’s modified Eagle’s medium (DMEM) and foetal bovine serum (FBS) were obtained from Biowest (Nuaillé, France).
7
Caspase Inhibitors in DNA Damage Response
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KU-0060648 was provided by GuideChem (Shanghai, China). The caspase-3 specific inhibitor z-DEVD-fmk and the general caspase inhibitor Z-VAD-fmk were purchased from Sigma Chemicals (Louis, MO). NU-7026 and NU-7441 were purchased from Calbiochem (San Diego, CA). p-DNA-PKcs (Thr 2609) antibody was purchased from Santa Cruz (Shanghai, China), All other antibodies utilized in this study were obtained from Cell Signaling Tech (Danvers, MA), as described previously [10 (link), 22 (link)]. The concentrations of agents applied and the treatment durations were chosen based on published literatures and results from pre-experiments.
8
Flow Cytometry Strategy for B Cell Subsets
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A flow cytometry gating strategy for B cell subsets was performed as previously described (21 (link)). All antihuman fluorochromes were obtained from BD Biosciences. Harvested PBMCs were stained for 30 min at 4°C using CD19 (APC-H7; HIB19), CD10 (PerCP-Cy5.5; HI10a), CD21 (PE-Cy7; B-ly4), CD27 (BV510; L128), CD95 (PE; DX2), and the corresponding isotype controls with or without caspase-3 (Z-DEVD-FMK [Sigma-Aldrich, St. Louis, MO]) and caspase-1 (VX-765 [MedChem Express, Monmouth Junction, NJ]) inhibitors. When conducting in vitro inhibitor experiments, PBMCs were transferred to preheated complete medium (Roswell Park Memorial Institute 1640/10% fetal calf serum) and treated with Z-DEVD-FMK (50 μM) or VX-765 (2 μM), then cultured at 37°C for 4 h before harvesting cells.
9
Evaluation of Apoptosis Markers in Cells
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CdCl2 was obtained from Sigma-Aldrich (Poole, Dorset, UK). Antibodies against Tom40, GAPDH, cytochrome c, Bax, BCL-xl, HO-1 and caspase 3 were obtained from Santa Cruz Biotechnology (Middlesex, UK). Horseradish peroxidase-conjugated goat anti-rabbit antibody was obtained from Bio-Rad laboratory (Hempstead, UK). Polyacrylamide (30 %) was purchased from Seven Biotech Ltd (Worcestershire, UK). Nitrocellulose membranes were purchased from Amersham Biosciences (Amersham, Bucks, UK). Caspase 3 and Calpain activity detection kits were obtained from Calbiochem (Nothingham, UK) and Promega (Southampton, UK), respectively. Annexin V-Cy3 Apoptosis detection Kit Plus (Cat # K202-25) was obtained from BioVision (Mountain View, CA, USA). Protoporphrin IX cobalt chloride (CoPPIX), SnPPIX, Z-DEVD-FMK, and all other chemicals were of the highest grade available and were obtained from Sigma-Aldrich (Poole, Dorset, UK).
10
Kinase Inhibitor and Caspase Assays
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OSI-027 and AT406 were provide by Selleck Co. (Shanghai, China). Caspase inhibitors including z-VAD-fmk and z-DEVD-fmk were purchased from Sigma (Shanghai, China). All kinase antibodies were purchased from Cell Signaling Tech (Denver MA). The other antibodies utilized in this study were obtained from Santa Cruz Biotech (Santa Cruz, CA).
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