Transaminase cii test

Manufactured by Fujifilm

Sourced in Japan

The Transaminase CII-Test is a lab equipment product manufactured by Fujifilm. Its core function is to measure the activity of the enzyme transaminase, which is important in the diagnosis and monitoring of liver diseases.

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28 protocols using transaminase cii test

Hepatic Enzyme Assays Protocol

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Hepatic functional parameters (alanine aminotransferase (ALT) and aspartate aminotransferase (AST) assays were conducted spectrophotometrically using commercially available kits (Transaminase CII-Test; Wako Pure Chemical Industries, Ltd., Osaka, Japan).

Shin M.R., Shin S.H, & Roh S.S. (2020). Diospyros kaki and Citrus unshiu Mixture Improves Disorders of Lipid Metabolism in Nonalcoholic Fatty Liver Disease. Canadian Journal of Gastroenterology & Hepatology, 2020, 8812634.

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Serum Biochemical Analysis in Rats

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Serum biochemical parameters were analyzed by the following methods. SUN measurements were determined by a colorimetric assay using a urease-indophenol method (Wako Pure Chemicals, Osaka, Japan). IGF-1 and glucose measurements were analyzed with an enzyme-linked immunosorbent assay (ELISA; MG100; R&D Systems, Minneapolis, MN, USA) and a colorimetric assay by the mutarotase-glucose oxidase method (Glucose CII-test; Wako), respectively. Insulin and glucagon were measured using ELISA kits (AKAIN-010T; Shibayagi, Gunma, Japan, and Wako, respectively). Cortisol was analyzed by an ELISA kit (KGE008; R&D Systems). AST and ALT were measured by the pyruvate oxidase-N-ethyl-N-(2-hydroxy-3-sulfopro-pyl)-m-toluidine method with a commercial kit (Transaminase CII-test; Wako). To measure renal tissue cAMP content, kidneys were prepared by homogenizing with HCl. Extracted cAMP levels were analyzed by using a cAMP radioimmunoassay kit (Yamasa, Chiba, Japan), and were corrected by protein concentration.
All biochemical parameters were measured according to the manufacturers’ instructions and are expressed as the mean ± standard deviation (SD) (n = 6 rats per treatment group).

Kugita M., Nishii K., Yamaguchi T., Suzuki A., Yuzawa Y., Horie S., Higashihara E, & Nagao S. (2017). Beneficial effect of combined treatment with octreotide and pasireotide in PCK rats, an orthologous model of human autosomal recessive polycystic kidney disease. PLoS ONE, 12(5), e0177934.

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Whole Blood Processing and ALT Assay

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Whole blood samples were obtained from the heart and serum was collected after centrifugation at 1000× g for 30 min. Alanine aminotransferase (ALT) was assayed with the Transaminase CII Test (Wako, Osaka, Japan).

Yamamotoya T., Nakatsu Y., Matsunaga Y., Fukushima T., Yamazaki H., Kaneko S., Fujishiro M., Kikuchi T., Kushiyama A., Tokunaga F., Asano T, & Sakoda H. (2017). Reduced SHARPIN and LUBAC Formation May Contribute to CCl4- or Acetaminophen-Induced Liver Cirrhosis in Mice. International Journal of Molecular Sciences, 18(2), 326.

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Hepatic Enzyme Assays Using Microplate

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Hepatic functional parameters aspartate aminotransferase (AST) and alanine aminotransferase (ALT) assays were measured with a microplate fluorescence reader using a commercial kit (transaminase CII-test from Wako Pure Chemical Industries Ltd., Osaka, Japan).

Lee J.A., Shin M.R., Kim M.J., Lee J.H., Park H.J, & Roh S.S. (2021). Protective Effects of Inflammation of Curcumae Longae Rhizoma 30% EtOH Extract on Acute Reflux Esophagitis Rats. BioMed Research International, 2021, 8854945.

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Portal Vein Blood ALT Assay

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A 0.5 ml of blood sample was collected from the portal vein. Plasma samples were assayed for ALT levels using a commercially available analytical kit (Transaminase CII-Test; Wako Pure Chemical Industries).

Lin S.H., Chung P.H., Wu Y.Y., Fung C.P., Hsu C.M, & Chen L.W. (2017). Inhibition of nitric oxide production reverses diabetes-induced Kupffer cell activation and Klebsiella pneumonia liver translocation. PLoS ONE, 12(5), e0177269.

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Liver Lipid Extraction and Analysis

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Liver lipids were extracted by the method described in the work of Folch et al. [18 (link)]. Triacylglycerol (TG), total cholesterol (T-cho), HDL-cholesterol, glutamic-oxaloacetic transaminase (GOT), and glutamic-pyruvic transaminase (GPT) in plasma and liver extracts were measured using test kits (Triacylglycerol E-Test, Cholesterol E-Test, HDL-Cholesterol E-Test, and Transaminase CII-Test, Wako Pure Chemical Industries, Osaka, Japan).

Yamada Y., Endou M., Morikawa S., Shima J, & Komatshzaki N. (2018). Lactic Acid Bacteria Isolated from Japanese Fermented Fish (Funa-Sushi) Inhibit Mesangial Proliferative Glomerulonephritis by Alcohol Intake with Stress. Journal of Nutrition and Metabolism, 2018, 6491907.

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Lipid and Liver Enzyme Profiling

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Plasma levels of triglyceride (TG), aspartate aminotransferase (AST), and alanine transaminase (ALT) were measured using the TG E-test (Wako Chemical, Osaka, Japan) and the transaminase CII-test (Wako Chemical), respectively. High-density lipoprotein cholesterol (HDL-chol) and low-density (LDL-chol) and very low-density lipoprotein-cholesterol (VLDL-chol) were assayed using HDL-C and LDL-C/VLDL-C Quantification Kit (BioVision, Milpitas, CA, USA).

Yamashita S., Hirashima A., Lin I.C., Bae J., Nakahara K., Murata M., Yamada S., Kumazoe M., Yoshitomi R., Kadomatsu M., Sato Y., Nezu A., Hikida A., Fujino K., Murata K., Maeda-Yamamoto M, & Tachibana H. (2018). Saturated fatty acid attenuates anti-obesity effect of green tea. Scientific Reports, 8, 10023.

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Determination of Hepatic Glutathione Levels

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Hepatic reduced glutathione (GSH) and oxidized glutathione (GSSG) levels were determined using the method of Anderson [14 (link)] and Raman et al. [15 (link)]. Briefly, 1 volume of liver tissue was homogenized in 9 volumes of 5% 5-sulfosalicylic acid, and centrifuged (10,000× g for 5 min at 4 °C). The supernatant was used in the 5,5′-dithiobis(2-nitrobenzoic acid)-glutathione reductase recycling assay to determine total glutathione and GSSG concentrations. GSH concentration was calculated from the difference between total glutathione and GSSG. Plasma ALT activity was measured using a commercial kit, Transaminase CII-test (Wako Pure Chemical Industries, Tokyo, Japan), according to the manufacturer’s instructions.

Nishimura N., Tanabe H., Komori E., Sasaki Y., Inoue R, & Yamamoto T. (2018). Transplantation of High Hydrogen-Producing Microbiota Leads to Generation of Large Amounts of Colonic Hydrogen in Recipient Rats Fed High Amylose Maize Starch. Nutrients, 10(2), 144.

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Evaluating EGCG and Eriodictyol for Myeloma Treatment

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Seven-week-old female BALB/c mice were obtained from Kyudo (Tosu, Japan). Mice were inoculated subcutaneously in the interscapular area with 1 × 10⁶ MPC-11 mouse myeloma cells. After the appearance of palpable tumours, mice were divided randomly into four groups with an even distribution of tumour sizes and injected i.p. daily with either saline alone, EGCG (15 mg/kg), eriodictyol (15 mg/kg), or with EGCG plus eriodictyol (both at 15 mg/kg) every 2 days. Tumor growth was measured with callipers, and the tumour volume calculated as (length × width²)/2.
Aminotransferase (AST) and alanine transaminase (ALT) activities were measured using the transaminase CII-test (Wako Pure Chemical Industries Ltd., Osaka, Japan) to assess heptatoxicity. Immunohistochemical staining and analysis were performed as previously described16 (link).

Kumazoe M., Fujimura Y., Hidaka S., Kim Y., Murayama K., Takai M., Huang Y., Yamashita S., Murata M., Miura D., Wariishi H., Maeda-Yamamoto M, & Tachibana H. (2015). Metabolic Profiling-based Data-mining for an Effective Chemical Combination to Induce Apoptosis of Cancer Cells. Scientific Reports, 5, 9474.

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Hepatic Function Assay Protocol

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Hepatic functional parameters, such as AST and ALT, were measured using specific assay kits and a microplate fluorescence reader (Transaminase CII-Test; Wako Pure Chemical Industries, Ltd., Osaka, Japan).

Shin M.R., Lee S.H, & Roh S.S. (2022). The Potential Hepatoprotective Effect of Paeoniae Radix Alba in Thioacetamide-Induced Acute Liver Injury in Rats. Evidence-based Complementary and Alternative Medicine : eCAM, 2022, 7904845.

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