Synergy ht take3 microplate reader

Manufactured by Agilent Technologies

Sourced in United States

The Synergy HT Take3 Microplate Reader is a compact and versatile instrument designed for absorbance-based assays. It provides fast and accurate measurements of samples in microplate format. The Synergy HT Take3 features a Xenon flash lamp, high-performance optics, and a sensitive photodetector to deliver reliable results across a wide range of applications.

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Lab products found in correlation

Mirneasy kit, by Qiagen (4 mentions) Superscript 4 vilo master mix, by Thermo Fisher Scientific (2 mentions) Taqman fast advanced master mix, by Thermo Fisher Scientific (1 mentions) Vilo master mix, by Thermo Fisher Scientific (1 mentions) Superscript 4 vilo master mix with ezdnase enzyme, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Microplate reader (6925 citations) Synergy HT (3550 citations) Synergy microplate reader (229 citations) Synergy HT reader (90 citations) Take3 (34 citations) Take3 microplate (4 citations)

5 protocols using synergy ht take3 microplate reader

Quantitative Gene Expression Analysis

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Real time RT-PCR was used to analyze mRNA expression. Briefly, total RNA was extracted
from lungs using a Qiagen kit (Cat #217004). The input RNA quality and concentration were
measured with a Synergy HT Take3 Microplate Reader (BioTek, Winooski, VT, USA), and cDNA
was prepared using SuperScript IV VILO Master Mix (Cat #11756500, Invitrogen). RT-PCR was
performed in duplicate using TaqMan™ Fast Advanced Master Mix (Cat #44-445-57,
ThermoFischer) for mRNA using an Mx3000P RT-PCR System (Stratagene, Santa Clara, CA, USA).
The primers for the PCR were purchased from Thermo Fisher Scientific (TaqMan). Results
were normalized using an internal control (Tuba1a).

Kitagawa A., Jacob C, & Gupte S.A. (2022). Glucose-6-phosphate dehydrogenase and MEG3 controls hypoxia-induced expression of serum response factor (SRF) and SRF-dependent genes in pulmonary smooth muscle cell. Journal of Smooth Muscle Research, 58, 34-49.

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Quantifying mRNA Expression in Tissues

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RT-PCR was used to analyze mRNA expression. Briefly, total RNA was extracted from lungs and human leukocytes using a Qiagen miRNEasy kit (catalog no.: # 217004). The quality and concentration of the input RNA were measured on the Synergy HT Take3 Microplate Reader (BioTek) and complementary DNA was prepared using SuperScript IV. VILO Master Mix (catalog no.: # 11756500, Invitrogen) for mRNA. Quantitative PCR (qPCR) was performed in duplicate using TaqMan Fast Advanced Master Mix (catalog no.: # 44-445-57) for mRNA using a Mx3000p Real-Time PCR System (Stratagene). The primers for the qPCR were purchased from Thermo Fisher Scientific/TaqMan. mRNA levels were normalized to internal control Tuba1a, and relative mRNA expression was reported.

Jacob C., Kitagawa A., Signoretti C., Dzieciatkowska M., D’Alessandro A., Gupte A., Hossain S., D’Addario C.A., Gupte R, & Gupte S.A. (2022). Mediterranean G6PD variant mitigates expression of DNA methyltransferases and right heart pressure in experimental model of pulmonary hypertension. The Journal of Biological Chemistry, 298(12), 102691.

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Quantitative mRNA Expression Analysis

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Real time PCR was used to analyze mRNA expression. Briefly, total RNA was extracted from lungs and human leukocytes using a Qiagen miRNeasy kit (Cat # 217004). The quality and concentration of the input RNA were measured on the Synergy HT Take3 Microplate Reader (BioTek) and cDNA was prepared using SuperScript™ IV VILO™ Master Mix with ezDNase™ Enzyme (Cat # 11766500, Invitrogen) for mRNA. Quantitative PCR was performed in duplicate using TaqMan™ Fast Advanced Master Mix (Cat # 44‐445‐57) for mRNA using a Mx3000p Real‐Time PCR System (Stratagene). The primers for the qPCR were purchased from Thermo Fisher Scientific/TaqMan. mRNA levels were normalized to internal control TUBA1A, and relative mRNA expression was reported.

D’Addario C.A., Lanier G.M., Jacob C., Bauer N., Hewes J.L., Bhadra A, & Gupte S.A. (2022). Differences in the expression of DNA methyltransferases and demethylases in leukocytes and the severity of pulmonary arterial hypertension between ethnic groups. Physiological Reports, 10(10), e15282.

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Quantitative Analysis of Aortic mRNA Expression

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Real-time PCR (RT-PCR) was used to analyze mRNA expression. Briefly, total RNA was extracted from aortas using a Qiagen miRNEasy kit (Cat # 217004). The quality and concentration of the input RNA were measured with a Synergy HT Take3 Microplate Reader (BioTek, Winooski, VT), and cDNA was prepared using SuperScript IV. VILO Master Mix (Cat # 11756500, Invitrogen) for mRNA. Quantitative PCR (qPCR) was performed in duplicate using TaqManTM Fast Advanced Master Mix (Cat # 44-445-57) for mRNA in a Mx3000p Real-Time PCR System (Stratagene, Santa Clara, CA). The primers for the qPCR were purchased from Thermo Fisher Scientific/TaqMan. mRNA levels were normalized to internal control Tuba1a, and relative mRNA expression was reported.

Signoretti C, & Gupte S.A. (2023). Studies in CRISPR-generated Mediterranean G6PD variant rats reveal G6PD orchestrates genome-wide DNA methylation and gene expression in vascular wall. bioRxiv.

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Quantitative Analysis of mRNA Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

Real-time RT-PCR technique was used to analyze mRNA expression. Briefly, total RNA was extracted from lungs using a Qiagen miRNEasy kit (217004). The input RNA quality and concentration were measured on the Synergy HT Take3 Microplate Reader (BioTek, Winooski, VT), and cDNA was prepared using SuperScript IV VILO Master Mix (11756500; Invitrogen) for mRNA. Quantitative PCR was performed in duplicate using TaqMan Fast Advanced Master Mix (44-445-57) for mRNA using a Mx3000p Real-Time PCR System (Stratagene, Santa Clara, CA). The primers for the QPCR were purchased from Thermo Fisher Scientific/TaqMan. Results for mRNA expression were normalized to internal control Tuba1a, and relative mRNA expression was determined using the ΔCt method.

Kitagawa A., Jacob C., Jordan A., Waddell I., McMurtry I.F, & Gupte S.A. (2021). Inhibition of Glucose-6-Phosphate Dehydrogenase Activity Attenuates Right Ventricle Pressure and Hypertrophy Elicited by VEGFR Inhibitor + Hypoxia. The Journal of Pharmacology and Experimental Therapeutics, 377(2), 284-292.

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