Fmoc amino acids

Fmoc-amino acids and resins in this study were purchased from GL Biochem Ltd. (Shanghai; HPLC-purified; purity > 99%, identified by mass spectra). Paclitaxel was obtained from Demochem Co (Shanghai, China). All other chemicals were obtained from Sigma-Aldrich unless otherwise noted. The human recombinant MMP2 was purchased from Biomol International, Inc (Plymouth, PA).

Rhein standard (purity > 98%) was purchased from Sangon Biotech (Shanghai, China). Fmoc‐amino acids were obtained from GL Biochem (Shanghai, China). All other materials used to synthesis Rh‐GFFYE and the other two compounds were obtained from Alfa (Beijing, China). Dulbecco's modified eagle medium (DMEM), penicillin/streptomycin were obtained from Life Technologies (Carlsbad, CA, USA). Calf serum was purchased from FuHeng BioLogy (Shanghai, China).H₂DCF‐DA was purchased from Beyotime Biotechnology Co., Ltd. (Shanghai, China). 4′,6‐Diamidino‐2‐phenylindole (DAPI) was purchased from Amy Jet Scientific Inc Co., Ltd. (Wuhan, China).

Fmoc-amino acids were obtained from GL Biochem (Shanghai). Doxorubicin hydrochloride was purchased from Aladdin. Commercially available reagents were used without further purification, unless noted otherwise. Nanopure water was used for all experiments. All other chemicals were reagent grade or better.

All Fmoc amino acids, rink amide-AM resin, HCTU, and HOBT were purchased from GL Biochem (Shanghai, China). The cell counting Kit-8 (CCK-8) and LDH cytotoxicity assay kit were order from Beyotime (Shanghai, China). Crystal violet and trypan blue were obtained from Sigma-Aldrich.

Rink Amide Resin (1.2 mmol/g) and 2-Cl-trityl chloride resin (1.1 mmol/g) were obtained from Nankai University resin Co., Ltd. Fmoc-amino acids and o-benzotriazol-1-yl-N,N,N′,N′-tetramethyluronium hexafluorophosphate (HBTU) were received from GL Biochem (Shanghai). Chemical regents and solvents were used as received from commercial sources. All the other starting materials were obtained from Alfa. Rapamycin was purchased from Alladin. 3-methyl adenine was purchased from J&K scientific. Cyanine5.5 NHS ester was purchased from Seebio. Horseradish peroxidase-conjugated goat antimouse IgG, IgG1, IgG2a, or IgG2b were obtained from Southern Biotechnologies (AL, USA). Recombinant mouse GM-CSF and IL-4 were purchased from Peprotech (Rocky Hill, USA). Mouse IL-5, IFN-γ, TNF-α, IL-2, IL-6, and IL-12 ELISA kits were obtained from Biolegend (CA, USA). Fluorochromelabeled anti-mouse monoclonal antibodies (PE-CD40, APC-CD80) and endotoxin free ovalbumin (OVA, endotoxins <1 EU/mg) were purchased from InvivoGen (CA, USA). Alum adjuvant was purchased from Pierce Biotechnology (IL, USA). Goat anti-mouse α-SMA, CD206, CD3 and iNOS antibodies were purchased from Abcam. Six to eight-week-old C57BL/6 mice were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. and maintained in specific pathogen-free conditions in the animal facility at the Nankai University, Tianjin, China.