Log In Sign up
The largest database of trusted experimental protocols

2 mercaptoenthanol

Manufactured by Thermo Fisher Scientific
Visit Supplier

2-Mercaptoethanol is a chemical compound used in various laboratory applications. It serves as a reducing agent, protecting sulfhydryl groups from oxidation and facilitating the maintenance of protein structure and function.

Automatically generated - may contain errors

Lab products found in correlation

Insulin, by Merck Group (2 mentions) Lsm 880 airyscan confocal microscope, by Zeiss (2 mentions) Poly ornithine, by Merck Group (2 mentions) Papain, by Worthington (2 mentions)

2 protocols using 2 mercaptoenthanol

1

Culturing and Transfecting Cortical Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols
For preparing WT and snph−/− cortical neuron cultures, E18 embryonic mice (sex: random) were used. After dissociation by papain (Worthington), neurons were re-suspended in plating medium (Neurobasal medium supplemented with 2% B-27, 0.5 mM GlutaMAX, 55 μM 2-Mercaptoenthanol (all from Thermo Fisher Scientific), 10% fetal bovine serum (HyClone) and 0.25 μg/ml insulin (Sigma-Aldrich)) and plated onto 12-mm coverslips (Deckgläser) coated with poly-ornithine (Sigma-Aldrich; 1:3 in PBS) in a 24-well tissue culture plate. After 24 hr of growing neurons in plating medium, half of the plating medium was replaced with the same amount of neuronal feeding medium (Neurobasal medium supplemented with 2% B-27, 0.5 mM GlutaMAX, and 5 μM 5-Fluoro-2-deoxyuridine) to inhibit glia proliferation. Neurons were fed every three days by aspirating half the medium and replacing it with the same amount of neuronal feeding medium. Neurons were transfected with various constructs at DIV7–9 using the calcium phosphate method and imaged at DIV14 with a Zeiss LSM880 Airyscan confocal microscope.
Li S., Xiong G.J., Huang N, & Sheng Z.H. (2020). The crosstalk of energy sensing and mitochondrial anchoring sustains synaptic efficacy by maintaining presynaptic metabolism. Nature metabolism, 2(10), 1077-1095.
+ Open protocol
+ Expand
2

Culturing and Transfecting Cortical Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols
For preparing WT and snph−/− cortical neuron cultures, E18 embryonic mice (sex: random) were used. After dissociation by papain (Worthington), neurons were re-suspended in plating medium (Neurobasal medium supplemented with 2% B-27, 0.5 mM GlutaMAX, 55 μM 2-Mercaptoenthanol (all from Thermo Fisher Scientific), 10% fetal bovine serum (HyClone) and 0.25 μg/ml insulin (Sigma-Aldrich)) and plated onto 12-mm coverslips (Deckgläser) coated with poly-ornithine (Sigma-Aldrich; 1:3 in PBS) in a 24-well tissue culture plate. After 24 hr of growing neurons in plating medium, half of the plating medium was replaced with the same amount of neuronal feeding medium (Neurobasal medium supplemented with 2% B-27, 0.5 mM GlutaMAX, and 5 μM 5-Fluoro-2-deoxyuridine) to inhibit glia proliferation. Neurons were fed every three days by aspirating half the medium and replacing it with the same amount of neuronal feeding medium. Neurons were transfected with various constructs at DIV7–9 using the calcium phosphate method and imaged at DIV14 with a Zeiss LSM880 Airyscan confocal microscope.
Li S., Xiong G.J., Huang N, & Sheng Z.H. (2020). The crosstalk of energy sensing and mitochondrial anchoring sustains synaptic efficacy by maintaining presynaptic metabolism. Nature metabolism, 2(10), 1077-1095.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!