1H NMR experiments were carried out on a Varian UNITY INOVA 500 spectrometer (Agilent Technologies, CA, USA) operating at 499.839 MHz. Spectra were acquired at 300 K using the standard 1D-NOESY pulse sequence for water suppression with a mixing time of 1 ms and a recycle time of 21.5 s. Spectra were recorded with a spectral width of 6000 Hz, a 90° pulse, and 128 scans. Prior to Fourier transformation the free induction decays (FID) were multiplied by an exponential weighting function equivalent to a line broadening of 0.5 Hz and zero-filled to 64 K. All spectra were phased and baseline corrected using MestReNova software (Version 9.0, Mestrelab Research S.L.). Chemical shifts were referred to the TSP single resonance at 0.00 ppm. 2D NMR 1H-1H COSY spectra were acquired with a spectral width of 6000 Hz in both dimensions, 4096 data points and 512 increments with 64 transients per increment.
Varian unity inova 500 spectrometer
The Varian UNITY INOVA 500 spectrometer is a high-performance nuclear magnetic resonance (NMR) instrument designed for analytical and research applications. It is capable of operating at a magnetic field strength of 500 MHz, providing high-resolution NMR spectroscopy capabilities. The UNITY INOVA 500 is a versatile instrument that can be used to analyze a wide range of chemical and biological samples.
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6 protocols using varian unity inova 500 spectrometer
Comprehensive NMR Characterization Protocol
1H NMR experiments were carried out on a Varian UNITY INOVA 500 spectrometer (Agilent Technologies, CA, USA) operating at 499.839 MHz. Spectra were acquired at 300 K using the standard 1D-NOESY pulse sequence for water suppression with a mixing time of 1 ms and a recycle time of 21.5 s. Spectra were recorded with a spectral width of 6000 Hz, a 90° pulse, and 128 scans. Prior to Fourier transformation the free induction decays (FID) were multiplied by an exponential weighting function equivalent to a line broadening of 0.5 Hz and zero-filled to 64 K. All spectra were phased and baseline corrected using MestReNova software (Version 9.0, Mestrelab Research S.L.). Chemical shifts were referred to the TSP single resonance at 0.00 ppm. 2D NMR 1H-1H COSY spectra were acquired with a spectral width of 6000 Hz in both dimensions, 4096 data points and 512 increments with 64 transients per increment.
1H NMR Spectroscopy Acquisition Protocol
NMR Spectroscopy Protocol for Characterization
1H-NMR experiments were performed on a Varian UNITY INOVA 500 spectrometer (Agilent Technologies, CA, USA) operating at 499.839 MHz. NMR spectra were acquired at 300 K using the standard one-dimensional NOESY pulse sequence for water suppression with a mixing time of 1 ms and a recycle time of 21.5 s. Spectra were recorded with a spectral width of 6000 Hz, a 90° pulse, and 128 scans. Prior to Fourier transformation, the free induction decays (FID) were multiplied by an exponential weighing function that was equivalent to a line broadening of 0.5 Hz and zero-filled to 64 K. All of the spectra were phased, and baselines were corrected using MestReNova software (Version 7.1.2, Mestrelab Research S.L.). 2D NMR 1H-1H COSY spectra were acquired with a spectral width of 6000 Hz in both dimensions, 4096 data points, and 512 increments with 64 transients per increment.
1H NMR Spectroscopy Protocol for Biochemical Analysis
NMR Spectroscopy of Milk and Urine
Spectroscopic Analysis of Organic Compounds
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