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Varian unity inova 500 spectrometer

Manufactured by Agilent Technologies
Sourced in United States

The Varian UNITY INOVA 500 spectrometer is a high-performance nuclear magnetic resonance (NMR) instrument designed for analytical and research applications. It is capable of operating at a magnetic field strength of 500 MHz, providing high-resolution NMR spectroscopy capabilities. The UNITY INOVA 500 is a versatile instrument that can be used to analyze a wide range of chemical and biological samples.

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6 protocols using varian unity inova 500 spectrometer

1

Comprehensive NMR Characterization Protocol

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1H NMR experiments were carried out on a Varian UNITY INOVA 500 spectrometer (Agilent Technologies, CA, USA) operating at 499.839 MHz. Spectra were acquired at 300 K using the standard 1D-NOESY pulse sequence for water suppression with a mixing time of 1 ms and a recycle time of 21.5 s. Spectra were recorded with a spectral width of 6000 Hz, a 90° pulse, and 128 scans. Prior to Fourier transformation the free induction decays (FID) were multiplied by an exponential weighting function equivalent to a line broadening of 0.5 Hz and zero-filled to 64 K. All spectra were phased and baseline corrected using MestReNova software (Version 9.0, Mestrelab Research S.L.). Chemical shifts were referred to the TSP single resonance at 0.00 ppm. 2D NMR 1H-1H COSY spectra were acquired with a spectral width of 6000 Hz in both dimensions, 4096 data points and 512 increments with 64 transients per increment.
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2

1H NMR Spectroscopy Acquisition Protocol

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The 1H NMR experiments were performed at 300 K on a Varian UNITY INOVA 500 spectrometer (Agilent Technologies, Inc., Santa Clara, CA, United States), operating at a frequency of 499.83 MHz. One-dimensional (1D) 1H NMR spectra were recorded using a standard pulse sequence (1D nuclear Overhauser effect spectroscopy), with pre-saturation during relaxation and mixing time for water suppression. Typically, 256 transients were acquired over a spectral width of 6000 Hz, with a total acquisition time of 1.5 s and a mixing time of 0.1 s. Prior to Fourier transformation, an exponential line-broadening factor of 0.3 Hz was applied to the free induction decays (FIDs). Then, spectra were phased and baseline-corrected, and the chemical shift scale was set by assigning a value of δ = 0.00 ppm to the signal for the internal standard TSP.
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3

NMR Spectroscopy Protocol for Characterization

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1H-NMR experiments were performed on a Varian UNITY INOVA 500 spectrometer (Agilent Technologies, CA, USA) operating at 499.839 MHz. NMR spectra were acquired at 300 K using the standard one-dimensional NOESY pulse sequence for water suppression with a mixing time of 1 ms and a recycle time of 21.5 s. Spectra were recorded with a spectral width of 6000 Hz, a 90° pulse, and 128 scans. Prior to Fourier transformation, the free induction decays (FID) were multiplied by an exponential weighing function that was equivalent to a line broadening of 0.5 Hz and zero-filled to 64 K. All of the spectra were phased, and baselines were corrected using MestReNova software (Version 7.1.2, Mestrelab Research S.L.). 2D NMR 1H-1H COSY spectra were acquired with a spectral width of 6000 Hz in both dimensions, 4096 data points, and 512 increments with 64 transients per increment.
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4

1H NMR Spectroscopy Protocol for Biochemical Analysis

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1H NMR experiments were carried out on a Varian UNITY INOVA 500 spectrometer (Agilent Technologies, CA, USA) operating at 499.839 MHz for proton. All the spectra were acquired at 300K using a standard 1D-NOESY pulse sequence for water presaturation with a mixing time of 1 ms and a recycle time of 6 sec. Spectra were recorded with a spectral width of 6000Hz, a 90° pulse, and 128 scans. Prior to Fourier transformation the free induction decays (FID) were multiplied by an exponential weighting function equivalent to a line broadening of 0.5 Hz and data were zero-filled to 64K. Chemical shifts were referred to the TSP single resonance at 0.00 ppm. All spectra were phased and baseline corrected using MestReNova software (Version 9.0.0, Mestrelab Research S.L.).
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5

NMR Spectroscopy of Milk and Urine

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1H NMR experiments were performed at 300 K with a Varian UNITY INOVA 500 spectrometer (Agilent Technologies, Inc., Santa Clara, CA, USA), operating at a frequency of 499.83 MHz. One-dimensional (1D) 1H NMR spectra were acquired using a standard pulse sequence (1D NOESY), with pre-saturation during relaxation and mixing time for water suppression. Both the milk and urine spectra were acquired with 128 scans with 64 k data points, a spectral width of 6000 Hz, a recycle time of 3.5 s, and a mixing time of 1 ms. A 0.3 Hz line-broadening factor was applied to each spectrum prior to Fourier transformation. After phased and baseline correction, the chemical shift scale was set by assigning a value of δ = 0.00 ppm to the signal for the internal standard TSP.
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6

Spectroscopic Analysis of Organic Compounds

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Optical rotations were recorded with a JASCO DIP 1000 digital polarimeter (JASCO, Tokyo, Japan). UV spectra were recorded on a JASCO V-530 spectrophotometer (JASCO, Tokyo, Japan), and CD spectra were recorded on a JASCO J-810 spectrometer (JASCO, Tokyo, Japan). IR spectra were obtained on a JASCO FT/IR 300-E spectrometer (JASCO, Tokyo, Japan). 1H-, 13C-, HSQC, and HMBC NMR experiments were recorded using a Varian Unity INOVA 500 spectrometer (Agilent Technologies, Inc., Santa Clara, CA, USA). HRESIMS were determined on Waters Synapt HDMS LC/MS mass spectrometer (Waters Corp., Milford, MA, USA). TLC was carried out on Merck silica gel F254-precoated glass and RP-18 F254S plates (Merck, Darmstadt, Germany). Medium pressure liquid chromatography (MPLC) was performed using LobarTM C18 column (10 × 240 mm, 40–63 μm, Merck, Darmstadt, Germany) and silica gel (40–63 μm, Merck). HPLC was performed on a Hewlett-Packard Agilent 1100 Series (Agilent Technologies, Inc., Santa Clara, CA, USA) HPLC System composed of a degasser, a binary mixing pump, a column oven and a DAD detector using Waters SunFire™ (Waters Corp., Milford, MA, USA) (4.6 × 150 mm, 5 μm) and SunFire™ Prep C18 column (10 × 150 mm, 5 μm) with acetonitrile (solvent A) and water containing 0.1% formic acid (solvent B).
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