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Phycoerythrin pe

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Phycoerythrin (PE) is a fluorescent protein derived from red algae. It is a bright, photostable fluorescent dye commonly used in flow cytometry and other fluorescence-based applications to label and detect target molecules.

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Lab products found in correlation

Anti mouse cd282 tlr2 phycoerythrin pe, by BioLegend (1 mentions) Macsquantify software version 2, by Miltenyi Biotec (1 mentions) Macsquant analyzer, by Miltenyi Biotec (1 mentions) β actin no 8457, by Cell Signaling Technology (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Anti cd45 pe, by BioLegend (1 mentions) Fluorescein isothiocyanate (fitc), by BioLegend (1 mentions) Facscalibur flow cytometer, by BD (1 mentions)

Close spelling variants of this product

Anti-PD-1-phycoerythrin (PE) Anti-mouse CD282 (TLR2) phycoerythrin (PE) Anti–CD19-phycoerythrin (PE)/Cy7 Phycoerythrin (PE) conjugated anti-mouse IL-4 antibody Phycoerythrin (PE)-conjugated streptavidin Phycoerythrin (PE)-conjugated anti-mouse CD86 Phycoerythrin (PE)-conjugated goat anti-mouse IgG CD45-phycoerythrin (PE; CD11c-phycoerythrin (CD11b-PE, APC-conjugated anti-CD83 and phycoerythrin (PE)-conjugated anti-CD86 mAbs

4 protocols using phycoerythrin pe

1

HLA-E Peptide Binding Analysis

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HLA-E restricted self-peptides, identified in this study, were synthesized and purchased from Thermo Fisher Scientific (Ulm, Germany) and dissolved in DMSO at a concentration of 100 mg/mL. For ELISA and affinity purification of soluble (s) HLA-E molecules the anti-HLA class I monoclonal antibody (mab) (clone W6/32) (AbD Serotec, Düsseldorf, Germany) or the mab anti-V5 (Life Technologies, Darmstadt, Germany) was used. The mab anti-β2m conjugated to horseradish peroxidase (HRP) (Dako, Hamburg, Germany) was used for detection in ELISA. The mab anti-HLA-E (clone 3D12) labeled with phycoerythrin (PE) (BioLegend, San Diego, USA) was used for flow cytometric analysis.
Kraemer T., Celik A.A., Huyton T., Kunze-Schumacher H., Blasczyk R, & Bade-Döding C. (2015). HLA-E: Presentation of a Broader Peptide Repertoire Impacts the Cellular Immune Response—Implications on HSCT Outcome. Stem Cells International, 2015, 346714.
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2

Flow Cytometry Analysis of TLR2 and TLR4 Expression

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MGFs (1 × 106 cells in 100 μL) were incubated with anti-mouse CD282 (TLR2) phycoerythrin (PE) (BioLegend, San Diego, CA, USA, Cat: 148604), anti-mouse CD284 (TLR4) phycoerythrin (PE) (BioLegend, Cat: 117605), or isotype control antibody phycoerythrin (PE) (BioLegend, Cat: 400508) and analyzed by flow cytometry using a MACSQuant analyzer and MACSQuantify software version 2.4 (Miltenyi Biotec, Tokyo, Japan).
Takayanagi Y., Kikuchi T., Hasegawa Y., Naiki Y., Goto H., Okada K., Okabe I., Kamiya Y., Suzuki Y., Sawada N., Okabe T., Suzuki Y., Kondo S., Ohno T., Hayashi J.I, & Mitani A. (2020). Porphyromonas gingivalis Mfa1 Induces Chemokine and Cell Adhesion Molecules in Mouse Gingival Fibroblasts via Toll-Like Receptors. Journal of Clinical Medicine, 9(12), 4004.
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3

Antibody Panel for Immune Phenotyping

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Phycoerythrin (PE)-, PE/Cy7, Alexa 647, allophycocyanin (APC)-, or APC/Cy7-conjugated anti-mouse CD4 (GK1.5), CD8 (53-6.7), CD25 (3C7), CD45RB (C363-16A), CD25 (3C7), CD44 (IM7), CD117 (2B8), TCRVβ (H57-597), TGF-β1 (TW7-16B4), FoxP3 (MF-14), and acetylated lysine (15G10; no. 623402) were purchased from BioLegend (San Diego, CA). Rabbit NAC1 (no. 4183), HDAC6 (no. 7612), and β-actin (no. 8457) antibodies were purchased from Cell Signaling (Beverly, MA). Rabbit anti-NAC1 antibody (ab29047) for immunoprecipitation was obtained from Abcam (Cambridge, MA). Mouse HDAC9 (no. sc-398003) and HDAC11 (no. sc-390737) antibodies were purchased from Santa Cruz Biotechnologies (Dallas, TX). Cycloheximide were purchased from Sigma-Aldrich Corporation (Sigma-Aldrich, St. Louis, MI).
Yang J.M., Ren Y., Kumar A., Xiong X., Das J.K., Peng H.Y., Wang L., Ren X., Zhang Y., Ji C., Cheng Y., Zhang L., Alaniz R.C., de Figueiredo P., Fang D., Zhou H., Liu X., Wang J, & Song J. (2022). NAC1 modulates autoimmunity by suppressing regulatory T cell–mediated tolerance. Science Advances, 8(26), eabo0183.
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4

Flow Cytometry Analysis of MSC Markers

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Surface markers were selected according to a previous report [31 (link)]. The cells were harvested by treatment with 0.05% trypsin-EDTA at the end of the third passage. For flow cytometry, the detached cells were washed and resuspended in PBS. Aliquots of 2 × 105 cells were labeled with fluorescein isothiocyanate (FITC) (BioLegend, San Diego, USA), phycoerythrin (PE) (BioLegend), and PE/CY5-conjugated monoclonal antibodies including an isotype control, anti-CD44-FITC (AbDSerotec, Oxford, UK), anti-CD29-PE/CY5, and anti-CD45-PE (BioLegend). Flow cytometry was performed with a FACSCalibur flow cytometer (Becton Dickinson, USA). In total, 10,000 events per sample were collected into listmode files and analyzed using WinMDI 2.9 analysis software.
Zhao H., He Z., Huang D., Gao J., Gong Y., Wu H., Xu A., Meng X, & Li Z. (2016). Infusion of Bone Marrow Mesenchymal Stem Cells Attenuates Experimental Severe Acute Pancreatitis in Rats. Stem Cells International, 2016, 7174319.
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