Human cd45 clone hi30

Manufactured by BioLegend

Human CD45 (clone HI30) is a cell surface glycoprotein that is expressed on all human leukocytes, including lymphocytes, monocytes, granulocytes, and their precursors. It functions as a protein tyrosine phosphatase, regulating a variety of cellular processes.

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Lab products found in correlation

Lsrfortessa, by BD (3 mentions) Mouse cd45 clone 30 f11, by BioLegend (3 mentions) Zombie yellow fixable viability kit, by BioLegend (3 mentions) Cd3 clone okt3, by BioLegend (2 mentions) Cd8 clone sk1, by BioLegend (2 mentions) Cd45ro clone uchl1, by BioLegend (2 mentions) Cd45ra hi100, by BioLegend (2 mentions) Cd4 clone a161a1, by BioLegend (2 mentions) Pd 1 clone mih4, by BD (2 mentions) Cd27 clone m t271, by BioLegend (1 mentions) Tim 3 clone f38 2e2, by BioLegend (1 mentions) Cd62l dreg 56, by BioLegend (1 mentions) Cd19 clone hib19, by BioLegend (1 mentions)

Spelling variants of this product

Clone HI30 (12 citations) CD45 (HI30; (8 citations) CD45 (clone HI30, (7 citations) Pacific Blue anti-human CD45 (clone HI30, (3 citations) PE-conjugated human CD45 antibody (clone HI30, (2 citations) Anti-human CD45 APC antibody (clone HI30, (2 citations)

3 protocols using human cd45 clone hi30

T Cell Phenotyping Protocol

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All samples were analyzed with an LSR Fortessa (BD Bioscience), and data were analyzed using FlowJo software (FlowJo). T cell phenotype was evaluated via Zombie Yellow Fixable Viability Kit (BioLegend), human CD45 (clone HI30; BioLegend), mouse CD45 (clone 30F11; BioLegend), CD3 (clone OKT3; BioLegend), CD4 (clone A161A1; BioLegend), CD8 (clone SK1; BioLegend), PD-1 (clone MIH4; BD Bioscience), CD45RO (clone UCHL1; BioLegend), and CD45RA (HI100; BioLegend).

Wang Y., Buck A., Grimaud M., Culhane A.C., Kodangattil S., Razimbaud C., Bonal D.M., Nguyen Q.D., Zhu Z., Wei K., O'Donnell M.L., Huang Y., Signoretti S., Choueiri T.K., Freeman G.J., Zhu Q, & Marasco W.A. (2021). Anti-CAIX BBζ CAR4/8 T cells exhibit superior efficacy in a ccRCC mouse model. Molecular Therapy Oncolytics, 24, 385-399.

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Evaluating CAR-T Cell Viability

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CAR-T expansion was evaluated using Zombie Yellow™ Fixable Viability Kit (BioLegend), human CD45 (clone HI30, BioLegend), and mouse CD45 (clone 30F11, BioLegend). All samples were analyzed with an LSR Fortessa (BD Bioscience) and data was analyzed using FlowJo software (FlowJo LLC).

Wang Y., Buck A., Piel B., Zerefa L., Murugan N., Coherd C.D., Miklosi A.G., Johal H., Bastos R.N., Huang K., Ficial M., Laimon Y.N., Signoretti S., Zhong Z., Hoang S.M., Kastrunes G.M., Grimaud M., Fayed A., Yuan H.C., Nguyen Q.D., Thai T., Ivanova E.V., Paweletz C.P., Wu M.R., Choueiri T.K., Wee J.O., Freeman G.J., Barbie D.A, & Marasco W.A. (2024). Affinity fine-tuning anti-CAIX CAR-T cells mitigate on-target off-tumor side effects. Molecular Cancer, 23, 56.

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Comprehensive T cell Phenotyping Protocol

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All samples were analyzed with an LSR Fortessa (BD Bioscience) and data were analyzed using FlowJo software (FlowJo LLC). T cell phenotype was evaluated via:Zombie Yellow Fixable Viability Kit (BioLegend), human CD45 (clone HI30, BioLegend), mouse CD45 (clone 30F11, BioLegend), CD3 (clone OKT3, BioLegend), CD4 (clone A161A1, BioLegend), CD8 (clone SK1, BioLegend), CD19 (clone HIB19, BioLegend), CD27 (clone M-T271, BioLegend), CD62L (DREG-56, BioLegend), PD-1 (clone MIH4, BD Bioscience), TIM-3 (clone F38-2E2, BioLegend), CD45RO (clone UCHL1, BioLegend), CD45RA (HI100, BioLegend).

Wang Y., Cho J.W., Kastrunes G., Buck A., Razimbaud C., Culhane A.C., Sun J., Braun D.A., Choueiri T.K., Wu C.J., Jones K., Nguyen Q.D., Zhu Z., Wei K., Zhu Q., Signoretti S., Freeman G.J., Hemberg M, & Marasco W.A. (2024). Immune-restoring CAR-T cells display antitumor activity and reverse immunosuppressive TME in a humanized ccRCC mouse model. iScience, 27(2), 108879.

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