Nω-nitro-L-arginine methyl ester (Fluka analytical), Griess reagent (Fluka analytical), and vanadium (III) chloride (Aldrich chemistry) were procured from Sigma-Aldrich Chemie, Germany. L-arginine was purchased from Himedia, India. Standard diagnostic kits for creatinine estimation were purchased from Span Diagnostic Pvt., Ltd., India. All the other reagents and solvents used were of analytical grade.
L arginine
Manufactured by HiMedia
Sourced in India
L-arginine is a naturally occurring amino acid that is essential for the body's normal functioning. It plays a key role in the production of nitric oxide, which helps to regulate blood flow and blood pressure. L-arginine is commonly used in various laboratory applications, such as cell culture and biochemical assays.
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Lab products found in correlation
3 protocols using l arginine
1
Nitric Oxide Quantification Assay
2
Optimizing Shoot Induction in Plant Tissue Culture
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The surface sterilized and antioxidant-treated nodal segments were inoculated vertically on Murashige and Skoog (MS; 1962 (link)) medium supplemented with 1.0, 2.0, 3.0, or 4.0 mg L−1 BAP or Kin (HiMedia®, Mumbai, India) for axillary shoot bud induction. Influence of 25 mg L−1 each of adenine sulfate, L-arginine, and citric acid and 50 mg L−1 ascorbic acid (all additives were procured from HiMedia®, India) on shoot growth and development were studied. Also, the effect of MS, ½ MS, WP (Lloyd and McCown 1981 ), or ½ WP on bud-breaking/shoot bud induction were optimized. The WP and MS media contained 2.0 and 3.0% (w/v) sucrose, respectively, and solidified with 0.8% (w/v) agar (Qualigens Fine Chemicals, Mumbai, India). The pH of medium with PGRs was adjusted to 5.8 ± 0.02 prior to autoclaving at 1.1 kg cm−2 pressure and 121°C temperature for 15 to 16 min. The cultures were initially incubated in dark for 2 to 3 d and thereafter shifted to growth room maintained at temperature 26 ± 2°C, photoperiod of 14 to 16-h with a light intensity of 40 to 50 μmol m−2 s−1 photon flux density (PFD) and relative humidity (RH) 60 ± 2%.
3
Extraction of Bacterial Whole Cell Proteins
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Bacterial whole cell proteins were extracted using previously described method[ 16,17,18 ] with some modifications. Pure culture of lactobacilli was inoculated in 20 ml MRS broth at 37°C for 24 hr, supplemented with 1% L-arginine (Hi-media, India) to delay autolysis. Cells from 20 ml of culture were harvested by centrifugation (7,000 × g); pellets were recovered and washed twice with PBS (Phosphate Buffer Saline) for removal of excess amount of MRS broth, and recentrifuged (10,000 × g). Bacterial cells then resuspended in 10 ml ice-cold acetone, allowed to withstand for 10 min, and collected by centrifugation (7,000 × g). The proteins were then extracted by incubating with 1.0 ml of 1% Sodium Dodecyl Sulfate (SDS) and stored at -20°C. Obtained whole cell proteins were quantified using Lowry's Method. [ 19 ]
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