Ultra High Temperature (UHT) treated milk was purchased from a local supermarket (Shanghai, China). It was confirmed negative for S. aureus, L. monocytogenes, and Salmonella spp. by culturing in Baird-Parker agar (BP; Beijing Land Bridge Technology Company Ltd, Beijing, China), PALCAM agar (Beijing Land Bridge Technology Company Ltd, Beijing, China), and bismuth sulfite agar (BS; Beijing Land Bridge Technology Company Ltd, Beijing, China), respectively. An overnight culture of S. aureus, L. monocytogenes, and Salmonella spp. (∼108 CFU/mL) was added to 1 mL of milk to final concentrations of 107, 106, 105, 104, 103, and 102 CFU/mL.
Palcam agar
Manufactured by Beijing Land Bridge Technology
Sourced in China
PALCAM agar is a selective culture medium used for the isolation and identification of Listeria monocytogenes in food samples. It inhibits the growth of most other bacteria, allowing Listeria to be more easily detected and enumerated.
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Lab products found in correlation
3 protocols using palcam agar
1
Evaluation of Pathogenic Bacterial Contamination in UHT Milk
2
Bacterial Enumeration in Shrimp Samples
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The bacterial enumeration method used in this study was the plating counting method according to previous studies (Belletti et al., 2013a (link); Wang et al., 2014 (link)). Briefly, shrimp samples were homogenized for 2 min in a stomacher (BagMixer400VW, Interscience, France). Then, a direct-plating procedure was used for the enumeration of V. parahaemolyticus, L. monocytogenes and indigenous microflora on shrimp samples. TCBS ager for V. parahaemolyticus, PALCAM agar (Beijing Land Bridge Technology Company Ltd., Beijing, China) for L. monocytogenes and Trypticase Soy Agar (TSA; Beijing Land Bridge Technology Company Ltd., Beijing, China) for indigenous microflora was used, respectively. The detection limit of the direct-plating was approximately 2 log10 CFU/g. Colonies were counted after the plates were incubated at 37°C for 24 h. Three replicates at each sampling time were done.
3
Isolation and Identification of L. monocytogenes
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The national standard GB 4789.30-2010 test method for food safety was used to isolate L. monocytogenes. After 24 h of culture at 30°C in 10 ml of LB1, 1 ml of each sample was transferred and sub-cultured in 10 ml of secondary Listeria enrichment broth 2 (LB2) (Beijing Land Bridge Technology CO., Ltd., China) for 24 h at 30°C. LB2 was obtained and streaked onto PALCAM agar (Beijing Land Bridge Technology CO., Ltd., China) and CHROMagar™ Listeria plates (Zhengzhou Biocell Biotechnology Co., Ltd., China) and then cultured for 24 h at 37°C. Five suspected colonies of L. monocytogenes were selected from each plate, streaked onto brain–heart infusion agar (Beijing Land Bridge Technology CO., Ltd., China) plates (in cases where no specific colonies exist, five single colonies with different morphologies were randomly chosen), and cultured for 24 h at 37°C. In accordance with the hospital's general sampling methods for assays in obstetrics and clinical gynecology, the cotton swabs or samples touched by inoculation loops were directly smeared onto China blue agar (Beijing Land Bridge Technology CO., Ltd., China) and blood agar (Beijing Land Bridge Technology CO., Ltd., China) plates for culturing without preliminary enrichment.
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