Sabouraud dextrose broth (sdb)

Manufactured by Liofilchem

Sourced in Italy

Sabouraud dextrose broth is a general-purpose culture medium used for the cultivation and isolation of fungi and yeasts. It provides the necessary nutrients, including dextrose, for the growth of these microorganisms.

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Lab products found in correlation

Sabouraud dextrose agar, by Liofilchem (7 mentions) Tryptic soy broth tsb, by Liofilchem (2 mentions) Sabouraud dextrose broth sdb, by Liofilchem (2 mentions) Tryptic soy agar, by Liofilchem (1 mentions) Luria bertani broth, by Formedium (1 mentions) Brain heart infusion agar, by Liofilchem (1 mentions) Mops acid, by Merck Group (1 mentions) Brain heart infusion broth, by Liofilchem (1 mentions) Rpmi 1640 medium, by Merck Group (1 mentions) L glutamine, by Merck Group (1 mentions) Bhi agar, by Liofilchem (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)

9 protocols using sabouraud dextrose broth (sdb)

Culturing Candida albicans and Streptococcus mutans

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In this study, Candida albicans ATCC 11225, stored at −80 °C in Sabouraud Dextrose broth (SDB, Liofilchem, Roseto degli Abruzzi, Italy) with 20% glycerol, and Streptococcus mutans DSM 20523, stored at the same conditions in Trypticase Yeast Extract broth (ISP, Liofilchem, Roseto degli Abruzzi, Italy), were used.
The fungus was subcultured in Sabouraud Dextrose agar (SDA, Liofilchem, Roseto degli Abruzzi, Italy), and, before each assay, one isolated colony was inoculated in SDB and grown aerobically at 37 °C overnight at 170 rpm. S. mutans was subcultured in Trypticase Yeast Extract agar (ISP agar, Liofilchem, Roseto degli Abruzzi, Italy), and, prior to the experiments, one isolated colony was inoculated in 10 mL of Yeast Extract broth (ISP, Liofilchem, Roseto degli Abruzzi, Italy) and grown anaerobically at 37 °C overnight. An aliquot of each culture (300 μL) was transferred into a new fresh liquid medium and grown under the same growth conditions until stationary growth phase was achieved.

Salgado H., Gomes A.T., Duarte A.S., Ferreira J.M., Fernandes C., Figueiral M.H, & Mesquita P. (2022). Antimicrobial Activity of a 3D-Printed Polymethylmethacrylate Dental Resin Enhanced with Graphene. Biomedicines, 10(10), 2607.

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Antifungal Potency Evaluation of Plant Extracts and Endodontic Irrigants

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The antifungal potency of the plant extract, the endodontic irrigant and the combination of plant extract with endodontic irrigants was evaluated using C. albicans (ATTC 10231, Liofilchem, Roseto degli Abruzzi, Italy).
The antifungal activities of the Soxhlet extracts of plants and the antifungal activities of endodontic irrigants were analyzed by a disk diffusion assay. The suspension of C. albicans was cultured for 48 hours in 1 mL of sterile Sabouraud dextrose broth (Liofilchem, Roseto degli Abruzzi, Italy) at 37°C, then adjusted to a turbidity of 0.5 on the McFarland scale (1.5×10⁸ cells/mL). This culture was used throughout the experiments. Mueller-Hinton agar (10 mL) was poured into Petri dishes, which were then inoculated with strains of yeast by the addition of 0.1 mL of cell culture media.
Sterile filter paper disks loaded with endodontic irrigants, plant extracts, and a combination of endodontic irrigants and plant extracts (10 mg/mL) were placed on top of Mueller–Hilton agar plates. These plates were incubated at 5°C for 2 hours to permit plant extract diffusion, and then they were incubated at 35°C for 24 hours. Disks that were only impregnated with extract solvent were used as a control. Inhibition zones, considered indicative of antibacterial activity, were measured using Vernier calipers and recorded.

Kameri A., Koçani F., Hashani Z., Kurteshi K., Kamberi B., Kurti A, & Haziri A. (2019). Antifungal and Synergistic Effects of the Ethyl Acetate Extract of Tanacetum vulgare (L.) Against Candida albicans. Medical Science Monitor Basic Research, 25, 179-186.

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Fungal and Bacterial Strain Culturing

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In this assay several fungal and bacterial strains were used, namely: C. albicans SC 5413, C. tropicalis ATCC 750, C. glabrata ATCC 2001, C. parapsilosis ATCC 9001; several clinical isolates from the biofilm/CEB collection; Pseudomonas aeruginosa DSM 22644; and Staphylococcus aureus ATCC 25923. For culturing, Sabouraud Dextrose Broth (SDB; Liofilchem) and Tryptic Soy Broth (TSB; Liofilchem, Italy), were used for fungi and bacteria, respectively. The broth was supplemented with 2% (w/v) agar (Liofilchem), when required, to obtain the respective solid media Sabouraud Dextrose Agar (SDA) and Tryptic Soy Agar (TSA). All yeast and bacteria strains, used in the study, were incubated at 37 °C for 18–24 h.

Fernandes L., Ribeiro H., Oliveira A., Sanches Silva A., Freitas A., Henriques M, & Rodrigues M.E. (2020). Portuguese honeys as antimicrobial agents against Candida species. Journal of Traditional and Complementary Medicine, 11(2), 130-136.

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Antimicrobial Evaluation of Fungal and Bacterial Strains

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Four fungal strains: Candida albicans (ATCC 90029), Candida parapsilosis (ATCC 22019), Candida krusei (ATCC 6258), and Candida tropicalis (ATCC 750) were used. The bacterial spp. used were Escherichia coli (ATCC 10536), Staphylococcus aureus (ATCC 25923), and Enterobacter aerogenesis (ATCC 13048), and three clinical isolates, namely, Providencia stuartii, P. aeruginosa, and Vibrio cholerae C06. Fungal and bacterial strains were obtained from the American Type Culture Collection (ATCC) while the clinical bacterial isolates were obtained from the Pasteur Institute Yaoundé (Cameroon). Mueller Hinton agar (MHA, Dominique Dutscher SAS) and Mueller Hinton broth (MHB, Dominique Dutscher SAS) were used for the activation of bacteria and antimicrobial assays, respectively. Sabouraud Dextrose agar (SDA, Liofilchem) and Sabouraud Dextrose broth (SDB, Liofilchem) were used for the activation of yeasts and antimicrobial assays, respectively.

Ndezo Bisso B., Njikang Epie Nkwelle R., Tchuenguem Tchuenteu R, & Dzoyem J.P. (2022). Phytochemical Screening, Antioxidant, and Antimicrobial Activities of Seven Underinvestigated Medicinal Plants against Microbial Pathogens. Advances in Pharmacological and Pharmaceutical Sciences, 2022, 1998808.

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Microbial Cultivation and Maintenance

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The test micro-organisms are shown in Table I. Bacterial strains were maintained in brain heart agar (Biolab, Budapest, Hungary) and yeast strains were maintained in Sabouraud dextrose agar (Liofilchem, Roseto degli Abruzzi, Italy) at 37 C. The liquid growth media used were Luria-Bertani (LB) broth (Formedium, Hunstanton, UK) for bacteria and Sabouraud dextrose broth (Liofilchem) for yeasts, at 37 C, 120 rpm, and constant temperature in an incubator shaker.

Cobrado L., Ramalho P., Ricardo E., Fernandes Â.R., Azevedo M.M, & Rodrigues A.G. (2022). Efficacy assessment of different time cycles of nebulized hydrogen peroxide against bacterial and yeast biofilms. The Journal of hospital infection, 127.

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Preparation of Microbial Growth Media

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Mueller-Hinton Broth (MHB), Mueller Hinton Agar (MHA), Brain Heart Infusion Broth (BHIB), Brain Heart Infusion Agar (BHIA), Sabouraud Dextrose Broth and Sabouraud Dextrose Agar from Liofilchem were prepared accordingly to manufacturer instructions. Phosphate buffered saline solution pH 7.4 (PBS) and RPMI 1640 medium supplemented with L-glutamine and buffered with MOPS acid, both from Sigma-Aldrich, were prepared accordingly to manufacturer instructions. Polyethylenimine (branched poly (ethyleneimine) solution, average Mw ~750.000, reference 181978; Sigma-Aldrich) stock solution at 25% (w/v) was used in the experiments.

Barros J., Dias A., Rodrigues M.A., Pina-Vaz C., Lopes M.A, & Pina-Vaz I. (2015). Antibiofilm and Antimicrobial Activity of Polyethylenimine: An Interesting Compound for Endodontic Treatment. The journal of contemporary dental practice, 16(6).

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Microbial Strain Preparation Procedure

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Enterococcus faecalis (ATCC 29212), stored at −80 °C in brain heart infusion broth (BHI broth, PanReac AppliChem, Darmstadt, Germany) with 20% glycerol, and Candida albicans (ATCC 11225) stored at the same temperature in Sabouraud dextrose broth (SDB, Liofilchem, Roseto degli Abruzzi, Italy) with 20% glycerol were used in this study.
The bacterium was subcultured in brain heart infusion agar (BHI agar, Liofilchem, Roseto degli Abruzzi, Italy) and, prior to the experiments, one isolated colony was inoculated in 10 mL of brain heart infusion broth and grown anaerobically at 37 °C overnight. Candida albicans was subcultured in Sabouraud dextrose agar (SDA, Liofilchem, Roseto degli Abruzzi, Italy) and, before each assay, one isolated colony was inoculated in SDB and grown aerobically at 37 °C overnight with stirring. An aliquot of each culture (300 μL) was transferred into a new fresh liquid medium and grown under the same growth conditions until the stationary growth phase. This culture was then used for the assay.

Correia B.L., Gomes A.T., Noites R., Ferreira J.M, & Duarte A.S. (2022). New and Efficient Bioactive Glass Compositions for Controlling Endodontic Pathogens. Nanomaterials, 12(9), 1577.

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Cultivating P. aeruginosa and C. albicans Strains

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Three reference strains of P. aeruginosa, two non-mucoid strains (PAO1 and UCBPP-PA14 (PA14)) and a mucoid strain (ATCC 39324), were used throughout this work. In addition, two clinical isolates of C. albicans (324LA/94, an oral isolate obtained from the culture collection of Cardiff Dental School (Cardiff, UK) and 547096, a urinary isolate obtained from the culture collection of the Biofilm Group of the Centre of Biological Engineering (Braga, Portugal)), and a reference strain, SC5314, were tested.
Prior to each assay, P. aeruginosa and C. albicans strains were subcultured from the frozen stock preparations onto Tryptic Soy Agar (TSA) and Sabouraud Dextrose Agar (SDA) plates, respectively. TSA and SDA were prepared from Tryptic Soy Broth (TSB; Liofilchem S.r.l., Roseto, Italy) or SDB (Liofilchem) supplemented with 1.2% (w/v) agar (Liofilchem). The plates were then incubated aerobically at 37 °C for 18–24 h.
Pure liquid cultures (pre-inocula) of P. aeruginosa were grown overnight in TSB, whereas C. albicans was maintained in SDB. For biofilm assays, 0.22 µm of filter-sterilized RPMI 1640 medium (Gibco^® by Life Technologies^TM, Grand Island, NY, USA) at pH 7.0 was used.

Grainha T., Magalhães A.P., Melo L.D, & Pereira M.O. (2020). Pitfalls Associated with Discriminating Mixed-Species Biofilms by Flow Cytometry. Antibiotics, 9(11), 741.

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Storage and Cultivation of Candida auris

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Candida auris NCPF 8971 was stored at −80 ± 2 °C in Sabouraud Dextrose Broth (SDB; Liofilchem, Roseto degli Abruzzi, Italy) with 20% (v/v) glycerol. Before each assay, C. auris was subcultured onto Sabouraud Dextrose Agar (SDA; Liofilchem) and incubated aerobically at 37 °C for 18–24 h. SDA plates were prepared from SDB supplemented with 2% (w/v) agar (Liofilchem).

Fernandes L., Ribeiro R., Costa R., Henriques M, & Rodrigues M.E. (2022). Essential Oils as a Good Weapon against Drug-Resistant Candida auris. Antibiotics, 11(7), 977.

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