Genetica cell line testing

All cell lines were incubated at 37°C in a humidified incubator supplied with 5% CO₂, maintained in subconfluent culture, and used for ≤ 20 passages. Thirty-two of thirty-four cell lines assayed for synergy in this study were authenticated by short tandem repeat genotyping (Genetica Cell Line Testing, LabCorp). HUVECs are pooled primary cells and have no reference STR profile. One of the cell lines used, KYSE150, was extinguished and not available for authentication. Re-analysis of the cell line screen data using VISAGE without data on KYSE150 cells did not significantly alter any of the results or conclusions of this study. All media was supplemented with 10% fetal bovine serum (FBS) and 2 mM glutamine, and lacked antibiotics unless otherwise specified for the selection of transduced cells. CCD-18co, MDA-MB-231, U-2 OS, A549, BT-20, MDA-MB-415, MDA-MB-157, U-87 MG, CFPAC1, HCT116, MDA-MB-468, T98G, MCF7, SW48, MDA-MB-453, MDA-MB-436, HT55, HT-29, HeLa, HEK293t and CCD-1112sk cells were grown in DMEM medium. C4–2, NCI-H1299, ZR-75–1, KYSE150, HCC38, HCT-15, 22RV1, LNCaP, AU565, CAL33, PC-3, LOVO and COLO205 cells were grown in RPMI-1640 medium. HUVEC cells were grown in EBM medium with EGM™−2 Bulletkit™.

BRAF-mutant melanoma cell lines, WM1341D, WM983B, 1205Lu, WM852, WM793, WM35, WM167 (gifted from Dr. Meenhard Herlyn, The Wistar Institute), A375 (purchased from ATCC), and 1205Lu derivatives, 1205Lu-M1 and 1205Lu-M2 (generated in this study), were maintained in DMEM with 1% penicillin-streptomycin, 1% L-glutamine, and 10% (v/v) FBS. BRAF-wildtype MeWo and NRAS-mutant SKMEL2 cell lines (both purchased from ATCC) were also maintained in the same culture medium as the BRAF-mutant cells. Cells were grown at 37°C under a humidified atmosphere of 5% CO_2. Cell lines (WM793, 1205Lu, 1205Lu-M1, and 1205Lu-M2) was authenticated by STR profiling (Genetica Cell Line Testing, LabCorp) and confirmed human origin.
In vivo mouse experiments were performed in compliance with an animal protocol approved by the Institutional Animal Care and Use Committee at the Center for Discovery and Innovation, Hackensack Meridian Health. Animal experiments were carried out with 10-12 weeks old NOD/SCID (NOD.Cg-Prkdc^scid/J) mice purchased from The Jackson Laboratory (Bar Harbor, ME) and maintained under standard pathogen free conditions.