About 1 μg of RNA was reverse transcribed using Qscript cDNA Supermix (QuantaBio). The cDNAs were diluted 10 times for expression analysis. qPCR on cDNA or ChIP-DNA was performed on 384-well plates on a QS5 system (Thermo Scientific) with GoTaq qPCR Master Mix (Promega). The fold change or percentage input of the samples was calculated based on relative standard curve method. Primers used in this study are listed in Supplementary Table S2 .
Qs5 system
Manufactured by Thermo Fisher Scientific
The QS5 system is a laboratory instrument designed for the analysis of biological samples. It provides a platform for various analytical techniques, such as spectroscopy and imaging. The QS5 system is capable of performing measurements and data processing, but its specific intended use should not be extrapolated beyond the provided factual information.
Automatically generated - may contain errors
Lab products found in correlation
Qscript cdna supermix, by Quantabio (3 mentions)
Gotaq qpcr master mix, by Promega (3 mentions)
Evagreen digital pcr supermix, by Bio-Rad (1 mentions)
Qx200 droplet digital pcr ddpcr system, by Bio-Rad (1 mentions)
Quantstudio design analysis software version 1, by Thermo Fisher Scientific (1 mentions)
3 protocols using qs5 system
1
RNA Reverse Transcription and qPCR Analysis
2
Reverse Transcription and qPCR Analysis
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One microgram of RNA was reverse transcribed using Qscript cDNA Supermix (QuantaBio). qPCR on cDNA or ChIP DNA was performed with GoTaq qPCR Master Mix (Promega) under the manufacturer’s instruction on a QS5 system (Thermo Scientific). The copy numbers were measured by predetermined standard curves. The relative expression or enrichment to control samples was calculated based on the ΔΔCt method. Quantitative digital droplet PCR was performed on a QX200 Droplet Digital PCR (ddPCR) System (Bio-Rad) with EvaGreen Digital PCR Supermix (Bio-Rad) under the manufacturer’s instructions. Primers used in this study are listed in Supplementary Data 1 .
3
Quantitative RT-PCR Analysis of Gene Expression
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1 ug of RNA was reverse transcribed using Qscript cDNA Supermix (QuantaBio). The cDNAs were diluted 3 times for expression analysis. qRT-PCR on cDNA or ChIP-DNA were performed on 384 well plates on a QS5 system (Thermo Scientific) with GoTaq qPCR Master Mix (Promega, Madison, WI). The fold change or percentage input of the samples was calculated using the QuantStudioTM Design & Analysis Software Version 1.2 (ThermoFisher Scientific) and represented as relative expression (ΔΔCt). All measurements were performed in triplicate. Primers used in this study are listed in Supplementary Table S1.
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