Hfl1 cell line

The HFL1 cell line was obtained from the ATCC (Manassas, VA, USA) and cultured in Eagle’s Minimum Essential Medium supplemented with 10% fetal bovine serum (Thermo Fisher) and a penicillin-streptomycin-glutamine mixture (Thermo Fisher) at 37°C.

The HFL1 cell line was purchased from ATCC (Manassas, VA, USA). HAM’s12 medium, L-glutamine, Penicillin-Streptomycin solution, 1,25(OH)₂D₃, Montelukast sodium, Leukotriene D4, goat anti-rabbit IGG, goat anti-mouse IGG, protease inhibitor cocktail, RIPA buffer and CYSLTR1 antibody were purchased from Merck (Darmstadt, Germany). Fetal bovine serum was purchased from Genos (Lodz, Poland). Beclomethasone 17-Propionate and primary antibodies such as E-cadherin (sc-8426), MMP-9 (sc-393859), ADAM33 (sc-514055), α-Actin (sc-32251), GAPDH (sc-47724) and Vimentin (sc-6260) were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Non-essential amino acids solution, TGFB1 Recombinant Human Protein, TRIzolTM Plus RNA Purification Kit, High-Capacity cDNA Reverse Transcription Kit, TaqManTM Gene Expression Master Mix, TaqMan™ gene expression assays: ACTA2 (Hs00426835_g1), MMP-9 (Hs009557562_m1), VIM (Hs00958111_m1), CysLTR1 (Hs00272624_s1), ADAM33 (Hs00905552_m1), CDH1 (Hs01023895_m1) and GAPDH (Hs02786624_g1) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Lastly, 4–20% ExpressPlus PAGE gel and MOPS Running buffer powder were purchased form GenSignal (Poznan, Poland).

The HFL1 cell line was obtained from the ATCC (Manassas, VA, USA) and cultured in Eagle’s Minimum Essential Medium supplemented with 10% fetal bovine serum (Thermo Fisher) and a penicillin-streptomycin-glutamine mixture (Thermo Fisher) at 37 °C.