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Human igfbp 2 quantikine elisa kit

Manufactured by R&D Systems
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The Human IGFBP-2 Quantikine ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) designed for the quantitative determination of human insulin-like growth factor-binding protein 2 (IGFBP-2) concentrations in cell culture supernates, serum, and plasma.

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Lab products found in correlation

Infinite 200 pro, by Tecan (1 mentions) Human igf 2 quantikine elisa kit, by R&D Systems (1 mentions) Human igf 1 quantikine elisa kit, by R&D Systems (1 mentions)

2 protocols using human igfbp 2 quantikine elisa kit

1

IGFBP-2 Quantification in Conditioned Media

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The medium was changed in both the induced CAF-like cells and the control MSCs to fresh DMEM. The conditioned media (CM) were collected after 48 h and analyzed using the Human IGFBP-2 Quantikine ELISA Kit (R&D Systems) according to the manufacturer’s instructions. The optical density of each well was read using a microplate reader (Infinite 200 PRO; Tecan, Mannedorf, Switzerland) at 450 and 570 nm wavelengths, and the concentration of each cytokine was calculated from the measured absorbance using a standard curve.
Shimizu M., Koma Y.I., Sakamoto H., Tsukamoto S., Kitamura Y., Urakami S., Tanigawa K., Kodama T., Higashino N., Nishio M., Shigeoka M., Kakeji Y, & Yokozaki H. (2021). Metallothionein 2A Expression in Cancer-Associated Fibroblasts and Cancer Cells Promotes Esophageal Squamous Cell Carcinoma Progression. Cancers, 13(18), 4552.
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2

ELISA-Based Quantification of IGF Biomarkers

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Total IGF1, IGF2, and IGFBP2 serum levels were measured using commercial ELISA kits (R&D, Cat # DG100, Human IGF-I Quantikine ELISA Kit; Cat # DG200, Human IGF-II Quantikine ELISA Kit; Cat # DGB200, Human IGFBP-2 Quantikine ELISA Kit). Total IGF1 and 2 measurements (free and bound) were determined by pre-treatment of the serum samples as per manufacturer protocol. All assays required the serum samples to be properly diluted (dilution factors were 100, 2000, and 50 respectively). All ELISA procedures and data analysis were performed in the same lab and followed the manufacturer protocols.
Yang J., Griffiths M., Nies M.K., Brandal S., Damico R., Vaidya D., Tao X., Simpson C.E., Kolb T.M., Mathai S.C., Pauciulo M.W., Nichols W.C., Ivy D.D., Austin E.D., Hassoun P.M, & Everett A.D. (2020). Insulin-like growth factor binding protein-2: a new circulating indicator of pulmonary arterial hypertension severity and survival. BMC Medicine, 18, 268.
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