Evos 5000 imaging system

Manufactured by Thermo Fisher Scientific

The EVOS 5000 imaging system is a high-performance microscope designed for live-cell and fixed-sample imaging. It features a motorized stage, advanced optics, and a user-friendly software interface to capture and analyze images in a range of microscopy techniques.

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Lab products found in correlation

Lipidtox deep red neutral lipid stain, by Thermo Fisher Scientific (1 mentions) Bodipy, by Thermo Fisher Scientific (1 mentions) Oil red o solution, by Merck Group (1 mentions)

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EVOS imaging system (62 citations) EVOS 5000 cell imaging system (2 citations)

2 protocols using evos 5000 imaging system

Lipid Staining during Cell Differentiation

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At different time points during the differentiation time course, plated cells were stained with live fluorescence dyes: BODIPY™ (boron-dipyrromethene, Invitrogen Cat. No. D3922,10 ng/mL), or LipidTOX™ Deep Red neutral lipid stain (Invitrogen, Cat. No. H34477, supplied as 1000X for standard assays) for 30 min at 37°C. Cells were incubated with the dyes in FluoroBrite phenol red-free DMEM medium (Gibco, Cat. No. A1896701) supplemented with 10% FBS and 1% penicillin–streptomycin for 30 min at 37°C in the dark, washed twice with warm PBS 1X and imaged in FluoroBrite medium using EVOS 5000 imaging system (ThermoFisher, Cat. No. AMF5000).

Porro G., Sarkis R., Obergozo C., Godot L., Amato F., Humbert M., Naveiras O, & Guiducci C. (2023). MarrowCellDLD: a microfluidic method for label-free retrieval of fragile bone marrow-derived cells. Scientific Reports, 13, 22462.

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Sertoli Cell Lipid Droplet Staining

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Canine primary Sertoli cells were seeded in a 35mm dish and cultured until 100% confluency. Cells were fixed with 4% paraformaldehyde for 30 min and incubated in 60% isopropanol solution for 5 min at room temperature. After removing isopropanol, the intracellular lipid droplets of Sertoli cells were stained with a filtered solution of Oil Red O solution 0.35% (Cat# O0625, Sigma-Aldrich, Louis, MO) in 2-isopropanol. The nucleus of cells was counterstained with Hematoxylin solution. Stained Sertoli cells were observed under the EVOS-5000 imaging system with 40X phase contrast objectives (Thermo Fisher Scientific, Waltham, MA).

Liberek K., Marszalek J., Ang D., Georgopoulos C, & Zylicz M. (1991). Escherichia coli DnaJ and GrpE heat shock proteins jointly stimulate ATPase activity of DnaK.. Proceedings of the National Academy of Sciences of the United States of America, 88(7), 2874-2878.

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