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Anti mouse cd28 pe

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Anti-mouse CD28 PE is a fluorescent-labeled antibody used to detect the presence of the CD28 protein on the surface of mouse cells. CD28 is a co-stimulatory molecule that plays a crucial role in the activation and regulation of T cells. The PE (Phycoerythrin) fluorescent label allows for the visualization and quantification of CD28-positive cells using flow cytometry or other fluorescence-based techniques.

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Lab products found in correlation

Anti mouse cd8 fitc, by Thermo Fisher Scientific (2 mentions) Anti mouse cd3 apc, by Thermo Fisher Scientific (2 mentions) Apc anti mouse cd3e, by Thermo Fisher Scientific (1 mentions) Facsaria 2, by BD (1 mentions)

3 protocols using anti mouse cd28 pe

1

Analyzing CD8+CD28- T Cells in Mice

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To determine the expression of CD8 + CD28-T cells in mice, FCM analysis was performed on isolated spleen cells using anti-mouse CD3, CD8, and CD28 monoclonal antibodies. The spleens were minced mechanically and lysed in a lymphocyte separation medium. The isolated spleen cells were washed and resuspended in PBS. Anti-mouse CD3 APC, anti-mouse CD8 FITC, and anti-mouse CD28 PE (eBioscience, San Diego, USA) were mixed at 4 0 C for 10 min in the dark. And then, the cell suspension was analyzed using FCM.
Yin N., Luo C., Wei L., Yang G., Bo L, & Mao C. (2024). The mechanisms of MicroRNA 21 in premature ovarian insufficiency mice with mesenchymal stem cells transplantation: The involved molecular and immunological mechanisms. Journal of Ovarian Research, 17, 75.
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2

Murine Splenocyte Immunophenotyping

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Mice spleens were mechanically minced. After lysing with lymphocyte separation medium, cells were resuspended in PBS. With 10-min incubation of anti-mouse CD3 APC, anti-mouse CD8 FITC, and anti-mouse CD28 PE (eBioscience, San Diego, USA) at 4 °C in the dark, the cell suspension was analyzed by FCM.
Yin N., Wu C., Qiu J., Zhang Y., Bo L., Xu Y., Shi M., Zhu S., Yang G, & Mao C. (2020). Protective properties of heme oxygenase-1 expressed in umbilical cord mesenchymal stem cells help restore the ovarian function of premature ovarian failure mice through activating the JNK/Bcl-2 signal pathway-regulated autophagy and upregulating the circulating of CD8+CD28− T cells. Stem Cell Research & Therapy, 11, 49.
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3

Cell Surface Marker Profiling for EL4, MOLM13, NB4 and TF1 Cell Lines

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Human and mouse cell surface markers were selected the basis of
homogeneity of expression as assessed by antibody staining profiles. Only cell
lines which showed expression of a particular cell surface marker in
>98% cells were chosen for analysis.
EL4 cells were independently stained and sorted on a FACS Aria flow
cytometer 8 days post transduction. Antibodies used in this study included:
eBioscience 17-5958-80 Anti-Mouse MHC Class I (H-2Kb) APC; eBioscience
12-0281-81 Anti-Mouse CD28 PE; eBioscience 11-0021-81 Anti-Mouse CD2 FITC;
eBioscience 11-0431-81 Anti-Mouse CD43 FITC; eBioscience 17-0031-81 Anti-Mouse
CD3e APC; eBioscience 17-0051-81 Anti-Mouse CD5 APC; Biolegend 124705 FITC
anti-mouse CD53; BD Biosciences 561974 APC conjugated anti-CD90.2 (Thy1.2); BD
Biosciences 560695 PE conjugated anti CD45.2
MOLM13, NB4 and TF1 cells were stained and sorted on a BD-FACS Aria II 8
days post transduction with the human sgRNA library. Antibodies used in this
study included: BD Pharmingen 555450 CD33-PE; BD Pharmingen 555394 CD13-PE; BD
Pharmingen 562371 CD15-PE.
Doench J.G., Hartenian E., Graham D.B., Tothova Z., Hegde M., Smith I., Sullender M., Ebert B.L., Xavier R.J, & Root D.E. (2014). Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation. Nature biotechnology, 32(12), 1262-1267.
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