Anti strepii

Manufactured by GenScript

Anti-strepII is a lab equipment product designed for protein purification. It functions as an affinity tag that can be used to isolate and purify recombinant proteins expressed in bacterial systems.

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Lab products found in correlation

Protein a g bead, by Santa Cruz Biotechnology (1 mentions) Bl21 de3, by Thermo Fisher Scientific (1 mentions)

2 protocols using anti strepii

Protein-protein Interaction Analysis Workflow

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Plasmids encoding human or murine MAG-ECD-Fc, Tie2-ECD-Fc, ANGPTL2-strep II and control vector were transiently co-transfected into 293T cells. The supernatant were collected 48 h after transfection and incubated with Protein A/G beads (Santa Cruz, sc2003) at 4 °C for 8 h, followed by washing with pre-chilled PBS with 0.1% NP-40 for 5 times. In the co-immunoprecipitation experiment for FYN and MAG, 293 T cells were transiently co-transfected with MAG (full-length)-FC, FYN-strepII and control vectors, lysed and incubated with Protein A/G beads (Santa Cruz, sc2003) or anti-strepII (GenScript, Cat#A00626-40) at 4 °C overnight, followed by washing with pre-chilled PBS with 0.1% NP-40 for 8 times. The immunoprecipitated proteins were examined by using the indicated antibodies for anti-strepII (GenScript, Cat#A00626-40) or anti-Fc (Sigma, Cat#12136) by western blot. The unedited agarose gel figures were showed in Additional file3

Chen L., Yu Z., Xie L., He X., Mu X., Chen C., Yang W., Tong X., Liu J., Gao Z., Sun S., Xu N., Lu Z., Zheng J, & Zhang Y. (2023). ANGPTL2 binds MAG to efficiently enhance oligodendrocyte differentiation. Cell & Bioscience, 13, 42.

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Bacterial Outer Membrane Protein Characterization

Cited 1 time

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All experiments were conducted in the E. coli K-12 strain AD202 (MC4100 ompT::kan) (45 (link)) or the E. coli B strain BL21(DE3) (Thermo Fisher). Darobactin, Polyphor 7 and 9 peptides, and MRL-494 were obtained from Kim Lewis (Northeastern University), Polyphor, and Merck, respectively. All antimicrobials were solubilized in water and stored as stock solutions at −20°C. pBpa was purchased from Bachem. Rabbit polyclonal antisera generated against an EspP C-terminal peptide, a peptide derived from the N terminus of the EspP β barrel, a BamA C-terminal peptide, and BamD have been described previously (22 (link), 34 (link), 46 (link)). Mouse monoclonal anti-His and anti-Strep II antisera were obtained from Genscript and Qiagen, respectively.

Peterson J.H., Doyle M.T, & Bernstein H.D. (2022). Small Molecule Antibiotics Inhibit Distinct Stages of Bacterial Outer Membrane Protein Assembly. mBio, 13(5), e02286-22.

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