Ab133602

Manufactured by Abcam

Sourced in China, United States

Ab133602 is a monoclonal antibody targeting the human Tyrosine Kinase-type Cell Surface Receptor Kit (CD117). This antibody is suitable for use in various immunoassay applications.

Automatically generated - may contain errors

Lab products found in correlation

Ab108985, by Abcam (2 mentions) Ab81289, by Abcam (2 mentions) Ab134045, by Abcam (2 mentions) Ab125011, by Abcam (2 mentions) Ab193349, by Abcam (1 mentions) Ab92726, by Abcam (1 mentions) Las 4000, by Cytiva (1 mentions) Luminata forte western hrp substrate, by Merck Group (1 mentions) Gapdh g8795, by Merck Group (1 mentions) Ab3368, by Abcam (1 mentions) Bxp 53, by Enzo Life Sciences (1 mentions) Ab8227, by Abcam (1 mentions) Biotinylated secondary antibody, by Vector Laboratories (1 mentions) Avidin biotin blocking kit, by Vector Laboratories (1 mentions) 3 3 diaminobenzidine peroxidase substrate kit, by Vector Laboratories (1 mentions) Progres c14 camera, by Jenoptik (1 mentions) Bx51 microscope, by Jenoptik (1 mentions) Bxp 21, by Abcam (1 mentions) Ecl kit, by Merck Group (1 mentions) Bca protein assay kit, by Keygen Biotech (1 mentions)

6 protocols using ab133602

Placental Alkaline Phosphatase Immunohistochemistry

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Immunohistochemistry was performed on 5 µm paraffin-embedded sections from term human placenta, without antigen retrieval. The placental alkaline phosphatase antibody (Abcam #ab133602) was applied at 1:1000 and detected with Lab Vision AEC substrate system (Thermo Scientific).

Hirschmugl B., Crozier S., Matthews N., Kitzinger E., Klymiuk I., Inskip H.M., Harvey N.C., Cooper C., Sibley C.P., Glazier J., Wadsack C., Godfrey K.M., Desoye G, & Lewis R.M. (2018). Relation of placental alkaline phosphatase expression in human term placenta to maternal and offspring fat mass. International journal of obesity (2005), 42(6), 1202-1210.

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Extracellular Vesicle Protein Profiling

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The pellet was resuspended in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) sample buffer, subjected to 12 or 10% SDS-PAGE, transferred to polyvinylidene difluoride membranes, and then probed with primary anti-CD63 (1:1000; #ab193349, Abcam, Shanghai, China), anti-CD9 (1:2000, #ab92726, Abcam, Shanghai, China), and anti-PLAP (1:5000, #ab133602, Abcam, Shanghai, China). After the membranes were washed with Tris-buffered saline Tween 20 (TBST), incubations with 1:4000 dilutions (v/v) of secondary antibodies were conducted for 2 h at 25 ± 1 °C. Protein expression was detected using the ECL Plus Western Blotting Detection System (ImageQuant LAS 4000 mini, GE Healthcare Life sciences, USA).

Zhao G., Yang C., Yang J., Liu P., Jiang K., Shaukat A., Wu H, & Deng G. (2018). Placental exosome-mediated Bta-miR-499-Lin28B/let-7 axis regulates inflammatory bias during early pregnancy. Cell Death & Disease, 9(6), 704.

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Western Blot Protocol for Protein Detection

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Unless otherwise stated, all Western blotting was performed using equipment from BioRad as previously described [30 (link)]. Primary antibodies were used to detect BCRP (BXP-53, 1:5000; Enzo Life Sciences, Farmingdale, NY), β-actin (ab8227, 1:2000, Abcam, Cambridge, MA), transferrin receptor 1 (TFR-1, ab108985, 1:5000 Abcam), placental alkaline phosphatase (PLAP, ab133602, 1:10,000, Abcam), multidrug resistance-associated protein 1 (MRP1, ab3368, 1:2000, Abcam), cluster of differentiation 34 (CD34, ab81289 1:10,000, Abcam), histone H2A (25785, 1:1000, Cell Signaling, Danvers, MA), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH, G8795, 1:1000, Sigma-Aldrich). HRP-linked secondary antibodies (anti-rabbit, anti-rat or anti-mouse, 1:2000 Sigma-Aldrich) were used to detect primary antibodies. After the addition of a Luminata Forte Western HRP substrate (Millipore, Billerica, MA), chemiluminescent protein-antibody complexes were visualized using a Fluorchem Imager (ProteinSimple, Santa Clara, CA). Semi-quantitative analysis of bands of the blots was performed using AlphaView Software (ProteinSimple).

Szilagyi J.T., Vetrano A.M., Laskin J.D, & Aleksunes L.M. (2017). Localization of the Placental BCRP/ABCG2 Transporter to Lipid Rafts: Role for Cholesterol in Mediating Efflux Activity. Placenta, 55, 29-36.

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Immunohistochemical Profiling of Tissue Samples

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For immunohistochemistry, tissue was embedded in paraffin and 5 μm thick sections prepared. After deparaffinization, tissue sections were quenched in 2% H₂O₂ (10 min, room temperature). Tissue sections were then blocked with an avidin/biotin blocking kit (Vector Laboratories, Burlingame, CA) followed by 5% serum corresponding to the source of the primary antibody. After 2 h at room temperature, tissues sections were incubated with primary antibodies to BCRP (BXP-21, 1:100; abcam), TFR-1 (ab108985, 1:200 Abcam), PLAP (ab133602, 1:1000, Abcam), or CD34 (ab81289 1:10,000, Abcam). After 16 h at 4°C, tissue sections were washed and incubated with biotinylated secondary antibodies for 60 min at room temperature (Vector Laboratories). Tissue sections were then stained using a 3,3′-diaminobenzidine peroxidase substrate kit (Vector Laboratories). After counterstaining with hematoxylin, tissue sections were dehydrated and imaged by light microscopy on a Olympus BX51 microscope (Waltham, MA) fitted with a ProgRes C14+ camera (Jenoptik, Jena, Germany). Negative controls for each secondary antibody are provided (Supplemental Fig 2).

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Comprehensive Protein Extraction and Western Blot Analysis

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Total proteins were extracted using RIPA lysis buffer with PMSF, phosphatase and protease inhibitors. Protein concentration was determined by BCA Protein Assay Kit (KeyGEN, China). The extracted proteins were separated to SDS-PAGE (8–10%) and electro-blotted on the polyvinylidene difluoride membrane. After blocked with 5% skim milk, the membranes were incubated with the primary antibodies against CD63 (1:1000, ab134045, Abcam, USA), CD9 (1:1000, ab236630, Abcam, USA), TSG101 (1:1000, ab125011, Abcam, USA), PLAP (1:10,000, ab133602, Abcam, USA), Calnexin (1:1000, ab133615, Abcam, USA), NLRP3 (1:1000, #15101, Cell Signaling Technology, USA), Cleaved Caspase-1 (1:1000, #4199, Cell Signaling Technology, USA), Cleaved GSDMD(1:1000, #36425, Cell Signaling Technology, USA), ASC (1:5000, 10500-1-AP, Proteintech, China), PDIA4 (1:1000, NBP2-90208, NOVUS, USA), DDIT4 (1:1000, ab191871, Abcam, USA) and β-Actin (1:1000, #4970, Cell Signaling Technology, USA) at 4 °C overnight. After washed with TBST, the membranes were incubated with secondary antibody (1:2000, Proteintech, China) for 1 h. Target proteins were detected using ECL kit (Millipore, USA) by an imaging system (Biolight, China).

Chen Q., He J., Liu H., Huang Q., Wang S., Yin A., Chen S., Shen X., Xiao Y., Hu H., Jiang J., Chen W., Wang S., Huang Z., Li J., Peng Y., Wang X., Yang X., Wang Z, & Zhong M. (2023). Small extracellular vesicles-transported lncRNA TDRKH-AS1 derived from AOPPs-treated trophoblasts initiates endothelial cells pyroptosis through PDIA4/DDIT4 axis in preeclampsia. Journal of Translational Medicine, 21, 496.

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Protein Expression Analysis by Western Blot

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All proteins were extracted using RIPA-PMSF methods and quantified through BCA kit (Solarbio, Beijing, China). Briefly, 20 μg of loaded protein was separated with 7.5%, 10% or 12.5% SDS‒PAGE (Epizyme, Shanghai, China), and then transferred onto PVDF membranes (Millipore, MA, USA). The membranes were blocked with 5% nonfat milk at room temperature for 1 h and incubated at 4°C overnight with the primary antibodies. The next day the membranes were incubated with the corresponding secondary antibody (Proteintech) for 2 h. Finally, the blots were visualized by Amersham Imager 600 system (GE, Boston, MA, USA). The relative quantitative values of the bands were measured by ImageJ software. Each assay was repeated three times.
Primary antibodies at 1:1000 dilution used for Western blotting were as follows: CD63 (ab134045; Abcam), TSG101 (ab125011; Abcam), PLAP (ab133602; Abcam), SPA (ab87674, Abcam; ab115791, Abcam), SPB (ab271345, Abcam; ab231551, Abcam), SPC (ABC99, Sigma-Aldrich; ab211326, Abcam), SPD (sc-25324, Santa Cruz Biotechnology; ab220422, Abcam), Sox9 (ab185966, Abcam), Bim (C34C5, CST), Bax (#2772, CST), Bcl2 (D17C4, CST), Cleaved Caspase-3 (1:500, 5A1E, CST) and tubulin (#2146, CST) as a control.

Chen P., Gu M., Wan S., Jiang X., Zhang F., Li Y., Zhou Q., Lu Y., Li L, & Wang X. (2023). Gestational Diabetes Mellitus Impedes Fetal Lung Development Through Exosome-Dependent Crosstalk Between Trophoblasts and Lung Epithelial Cells. International Journal of Nanomedicine, 18, 641-657.

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