Glycerine

Glycerine (>99.8%), Folin–Ciocalteau reagent, and K₂S₂O₈ were obtained from Chempur (Piekary Śląskie, Poland). Ethanol (>99.8%) was obtained from Honeywell (Charlotte, NC, USA). Tyrosinase from Agaricus bisporus, 3,4-dihydroxy-l-phenylalanine (L-DOPA), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), kojic acid (≥98.5% purity), quercetin (>95% purity), gallic acid (>97.5%), Dulbecco’s phosphate buffered saline (DPBS), Dulbecco’s modified Eagle’s medium (DMEM), bovine serum albumin (BSA), neutral red solution in DPBS (3.3 g/L) were purchased from Sigma Aldrich (Merck, Darmstadt, Germany). Fetal bovine serum (FBS) was obtained from Pan-Biotech (Aidenbach, Germany). The solvents used for phytochemical studies by HPLC-MS (water, acetonitrile, and formic acid) were purchased from Merck (Darmstadt, Germany).

The following chemical reagents were used to produce the nanocomposites: chitosan (high molecular weight: 310,000–375,000 Da, degree of deacetylation >75%) from shrimp shells, (Sigma-Aldrich, Saint Louis, MI, USA), sodium alginate (Sigma-Aldrich), acetic acid (Chempur, 99.5%), glycerine (99.5%, Chempur), deionized water, extra virgin olive oil, turmeric, ethanol 96% p.a. grade.
The following microbial strains were used for microbiological testing: Escherichia coli (DSMZ 1116), Aspergillus fumigatus (LOCK 042600), Penicillium expansum (LOCK 0535). The growth mediums used in the experiment: Mueller Hinton Lab-Agar (BIOMAXIMA), DRBC Lab-Agar (BIOMAXIMA).