Wt c57bl 6j mice

All WT C57BL/6J mice and Nr2f6^flox/flox mice were purchased from Gempharmatech Co., Ltd (Nanjing, China). Pdgfra-Cre mice were generously provided by Prof. Tong-jin Zhao from Fudan university [17 ]. B6.Cg-Gt(ROSA)26Sor^{tm9(CAG-tdTomato)Hze}/J (R26-LSL-tdTomato; stock no. 007909) were purchased from the Jackson Laboratory [18 (link)]. Nr2f6^flox/flox mice, containing loxp sites flanking exon 2 of the Nr2f6 gene, were generated using CRISPR-Cas9 technology. Preadipocytes specific Nr2f6 knockout mice (NR2F6-PKO) were obtained by crossing Nr2f6^flox/flox mice with Pdgfra-Cre mice. The mice were housed at 21 ± 1 °C with a humidity of 50 % ± 5 %, unless otherwise specified, with a 12-hour light–dark cycle with ad libitum access to food and water. Chow diets were purchased from Shanghai Pulutong Biotechnology (P1101F). For diet-induced obesity studies, mice were fed with HFD (D12492, 60 % fat, Research Diets, Inc). Body weights of these mice were measured weekly. Body fat and lean mass were quantified using an NMR analyzer (Minispec LF90II; Bruker Optics). All studies involving animal experiments were approved by the Fudan University Shanghai Medical College Animal Care and Use Committee (No. 20230301-045) and followed the National Institutes of Health guidelines on the care and use of animals.