B6 cg tg krt1 15 egfp 2cot j

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B6.Cg-Tg [Krt1-15-EGFP] 2Cot/J is a transgenic mouse strain that expresses enhanced green fluorescent protein (EGFP) under the control of the keratin 1-15 (Krt1-15) promoter. This model is useful for visualizing and studying keratinocytes and other epithelial cell types.

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Fvb ncrl mice, by Charles River Laboratories (1 mentions)

2 protocols using b6 cg tg krt1 15 egfp 2cot j

Two-stage Carcinogenesis Protocol for Skin Papilloma

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All animal experiments were approved by the NC State University Institutional Animal Care and Use Committee (IACUC). FVB/NCrl mice were obtained from Charles River laboratories (strain 207) and K15EGFP mice from The Jackson Laboratory (B6.Cg-Tg [Krt1-15-EGFP] 2Cot/J, Stock number 005244).
Skin papillomas were induced in transgenic mice overexpressing CDK4 in the epidermis (K5CDK4) by the two-stage carcinogenesis protocol as we previously described (27 (link),28 (link)). Briefly, groups of 20 mice each (K5CDK4 and wild type siblings) were initiated at day 1 after birth by topical application of 50 mg of DMBA in 50 ml of acetone on the mouse back. At day 14, mice received 2.5 mg of TPA in 200 ml of acetone twice a week for 20 weeks. Skin papillomas were collected at the end of the experiment at 30 weeks. Immunohistochemical analysis of skin tumors was performed on paraffin-embedded sections from mouse skin papillomas as described below.

Kim S.H., Sistrunk C., Miliani de Marval P.L, & Rodriguez-Puebla M.L. (2017). Characterization of hair-follicle side population cells in mouse epidermis and skin tumors. Oncology Letters, 14(6), 6497-6504.

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Genetic Labeling of Mouse Ocular Cells

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Animal care and use conformed to the Association for Research in Vision and Ophthalmology (ARVO) statement for the use of animals in ophthalmic and vision research. The mouse strains B6.Cg-Tg(Krt1–15-EGFP)2Cot/J (referred to as Krt15-GFP) (Fuchs et al., 2013 (link)), Gt(ROSA)26Sortm1(CAG-Brainbow2.1)Cle/Jand B6.Cg-Ndor1Tg(UBC-Cre^ERT2)1Ejb/2J (referred to as UBC-Cre^ERT2) were from The Jackson Laboratory (Bar Harbor, ME). The mouse strains: C57BL/6JOlaHsd (referred to as C57BL/6), NOD.CB17-Prkdcscid/NCrHsd (referred to as Nod-Scid), C.B-17/IcrHsd-Prkdcscid (referred to as Scid) and Hsd:Athymic Nude-Foxn1nu (referred to as Nude) and their controls BALB/cOlaHsd (referred to as BALB) were from Envigo RMS Ltd (Israel). UBC-Cre^ERT2; Brainbow^2.1 homozygous mice were generated by crossing UBC-Cre^ERT2 with R26R-Brainbow^2.1. Both male and female age-matched mice from 7 to 14 weeks of age were used for all experiments in this study. Littermates of the same genotype, sex, and age were randomly assigned to experimental groups. All mice were housed under specific-pathogen-free SPF conditions at the Technion animal facilities.

Altshuler A., Amitai-Lange A., Tarazi N., Dey S., Strinkovsky L., Hadad-Porat S., Bhattacharya S., Nasser W., Imeri J., Ben-David G., Abboud-Jarrous G., Tiosano B., Berkowitz E., Karin N., Savir Y, & Shalom-Feuerstein R. (2021). Discrete limbal epithelial stem cell populations mediate corneal homeostasis and wound healing. Cell stem cell, 28(7), 1248-1261.e8.

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