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P aurka thr288

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P-AURKA (Thr288) is a primary antibody that detects Aurora kinase A (AURKA) phosphorylated at threonine 288. AURKA is a serine/threonine protein kinase that plays a crucial role in cell cycle regulation and mitotic spindle assembly.

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Lab products found in correlation

P s6ser240 244, by Cell Signaling Technology (2 mentions) Aurka, by Cell Signaling Technology (2 mentions) P 4e bp1 thr37 46, by Cell Signaling Technology (2 mentions) P akt ser473, by Cell Signaling Technology (2 mentions) Cleaved parp asp214, by Cell Signaling Technology (2 mentions) Sds page gel, by Thermo Fisher Scientific (2 mentions) Pvdf membrane, by Merck Group (2 mentions) Bcl2 associated x (bax), by Cell Signaling Technology (2 mentions) Bcl 2, by Cell Signaling Technology (2 mentions) β actin, by Cell Signaling Technology (2 mentions) Nitrocellulose membrane, by Merck Group (1 mentions) Ha tag, by Merck Group (1 mentions) Nanog, by Abcam (1 mentions) C myc, by Santa Cruz Biotechnology (1 mentions) β actin, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

AURKA (26 citations) P-AURKA (5 citations)

4 protocols using p aurka thr288

1

Comprehensive Protein Analysis Workflow

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Proteins were extracted using RIPA buffer (50 mM Tris-HCl pH 7.5, 150 mM
NaCl, 0.1% sodium deoxycholate, 0.1% SDS, 1 mM EDTA pH 8.0,
1% NP-40) containing proteinase (Roche) and phosphatase (Roche)
inhibitor cocktails. Samples were resolved using 4–12% SDS-PAGE
gels (Life Technologies) and transferred to PVDF membranes (Millipore).
Membranes were probed overnight on a 4°C shaker with primary antibodies
(1:1,000 dilution unless indicated) recognizing the following proteins: p-AKT
(Ser473) (9271, Cell Signaling), AKT (4691, Cell Signaling), p-S6 (Ser240/244)
(5364, Cell Signaling, 1:20,000), p-4E-BP1 (Thr37/46) (2855, Cell Signaling),
p-AURKA (Thr288) (3079, Cell Signaling), AURKA (4718, Cell Signaling), Cleaved
PARP (Asp214) (9541, Cell Signaling), BCL2 (2870, Cell Signaling), BAX (2772,
Cell Signaling), MYC (ab32072, Abcam), and β-actin (3700, Cell
Signaling).
Donnella H.J., Webber J.T., Levin R.S., Camarda R., Momcilovic O., Bayani N., Shah K.N., Korkola J., Shokat K.M., Goga A., Gordan J.D, & Bandyopadhyay S. (2018). Kinome rewiring reveals AURKA limits PI3K-pathway inhibitor efficacy in breast cancer. Nature chemical biology, 14(8), 768-777.
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2

Comprehensive Protein Analysis Workflow

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteins were extracted using RIPA buffer (50 mM Tris-HCl pH 7.5, 150 mM
NaCl, 0.1% sodium deoxycholate, 0.1% SDS, 1 mM EDTA pH 8.0,
1% NP-40) containing proteinase (Roche) and phosphatase (Roche)
inhibitor cocktails. Samples were resolved using 4–12% SDS-PAGE
gels (Life Technologies) and transferred to PVDF membranes (Millipore).
Membranes were probed overnight on a 4°C shaker with primary antibodies
(1:1,000 dilution unless indicated) recognizing the following proteins: p-AKT
(Ser473) (9271, Cell Signaling), AKT (4691, Cell Signaling), p-S6 (Ser240/244)
(5364, Cell Signaling, 1:20,000), p-4E-BP1 (Thr37/46) (2855, Cell Signaling),
p-AURKA (Thr288) (3079, Cell Signaling), AURKA (4718, Cell Signaling), Cleaved
PARP (Asp214) (9541, Cell Signaling), BCL2 (2870, Cell Signaling), BAX (2772,
Cell Signaling), MYC (ab32072, Abcam), and β-actin (3700, Cell
Signaling).
Donnella H.J., Webber J.T., Levin R.S., Camarda R., Momcilovic O., Bayani N., Shah K.N., Korkola J., Shokat K.M., Goga A., Gordan J.D, & Bandyopadhyay S. (2018). Kinome rewiring reveals AURKA limits PI3K-pathway inhibitor efficacy in breast cancer. Nature chemical biology, 14(8), 768-777.
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3

Western Blot Analysis of Stem Cell Markers

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Cells were lysed in RIPA (radioimmunoprecipitation assay) buffer and the protein concentrations determined by the Bradford assay. Equal amounts of cell extracts were subjects to electrophoresis on sodium dodecyl sulphate–polyacrylamide gel and blotted onto nitrocellulose membrane (Millipore, Merck, Shanghai, China). After protein transfer, the membranes were blocked and then incubated with glyceraldehyde 3-phosphate dehydrogenase (Ambion, ThermoFisher Scientific), β-actin (Santa Cruz Biotechnology, Gene Company Ltd), p-AURKA (Thr288; Cell Signaling Technology, Gene Company Ltd), AURKA (Upstate, Gene Company Ltd), HA-tag (Sigma), c-Myc (Santa Cruz Biotechnology), SOX2 (Epitomics, Abcam, Hang Zhou, China) and Nanog (Epitomics, Abcam) primary antibodies at 4 ° overnight. The membranes were then incubated for 1 h at room temperature with the appropriate secondary antibodies. Proteins were detected with an enhanced chemiluminescence kit (Pierce, ThermoFisher Scientific).
Yang N., Wang C., Wang Z., Zona S., Lin S.X., Wang X., Yan M., Zheng F.M., Li S.S., Xu B., Bella L., Yong J.S., Lam E.W, & Liu Q. (2017). FOXM1 recruits nuclear Aurora kinase A to participate in a positive feedback loop essential for the self-renewal of breast cancer stem cells. Oncogene, 36(24), 3428-3440.
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4

Immunoblotting of OFD1, p-AURKA, p-AURKB

Cited 1 time
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The Immunoblotting was performed as described previously [20 (link)]. The primary antibodies used for immunoblotting were as follows: rabbit polyclonal anti-OFD1 (Proteintech, 22851–1-AP), mouse monoclonal anti-β-actin (Santa Cruz, sc-47778), rabbit monoclonal p-AURKA Thr288 (Cell Signaling, #3079) and rabbit monoclonal p-AURKB Thr232 (Cell Signaling, #2914).
Ka H.I., Cho M., Kwon S.H., Mun S.H., Han S., Kim M.J, & Yang Y. (2023). IK is essentially involved in ciliogenesis as an upstream regulator of oral-facial-digital syndrome ciliopathy gene, ofd1. Cell & Bioscience, 13, 195.
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