The ELISA Detection kit (Roche, Palo Alto, CA, USA) was used to detect MMP-1 and Smad7 in HSFs with different treatments. Briefly, following the indicated treatments, the cytoplasmic histone/DNA fragments from cells were extracted and bound to immobilized anti-histone antibody. Subsequently, the peroxidase-conjugated anti-DNA antibody was used for the detection of immobilized histone/DNA fragments The antibodies used were from the ELISA Detection kit (Roche). Following the addition of a substrate for peroxidase, the spectrophotometric absorbance of the samples was determined using the Dynatech MR5000 plate reader at 405 nm (Dynatech Laboratories, Chantilly, VA, USA).
Elisa detection kit
Manufactured by Roche
Sourced in China, United States
The ELISA detection kit is a laboratory instrument used for conducting enzyme-linked immunosorbent assay (ELISA) tests. The kit provides the necessary components to perform quantitative or qualitative analysis of target analytes in a sample through antigen-antibody interactions and color-based detection.
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Lab products found in correlation
3 protocols using elisa detection kit
1
Quantifying MMP-1 and Smad7 in HSFs
2
Quantifying Cisplatin-Induced Apoptosis
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The cisplatin-induced rate of apoptosis in both control and cisplatin-resistant cell lines were detected using an ELISA detection kit (Roche Applied Sciences), according to the manufacturer's instructions. Briefly, cells were cultured in 96-well plates at a density of 1×104 cells/well for 48 h and the test agents were added to the culture medium containing 10% FBS. After 24 h, the cytoplasm of cells in the control (untreated) and treatment (cisplatin-treated) groups was transferred to streptavidin-pre-coated 96-well plates, which had been previously incubated with a biotinylated histone antibody and peroxidase-tagged mouse anti-human DNA for 2 h at room temperature. The absorbance was measured at 405 nm using an ELX-800 ELISA plate reader (Bio-Tek, Winooski, VA, USA). In addition, the apoptosis rate induced by BrMC treatment under identical conditions was estimated using the same method.
3
Cell Apoptosis Quantification via ELISA
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The cell apoptosis histone DNA ELISA detection kit (Roche, Shanghai, China) [34 (link)] was applied to quantify cell apoptosis using the manufacturer’s protocol. ELISA OD at 450 nm was tested to indicate cell apoptosis intensity.
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