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Fv31s swpad software

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The FV31S-SWPAD software is a digital imaging software developed by Olympus for use with their FluoView confocal microscope systems. The software provides the core functionality for capturing, processing, and analyzing digital microscopy images.

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Lab products found in correlation

Fv10 asw software, by Olympus (2 mentions) Fv3000 fluoview inverted confocal microscope, by Olympus (2 mentions) Fv1000 upright confocal microscope, by Olympus (2 mentions)

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FV31S software (4 citations)

2 protocols using fv31s swpad software

1

In vivo Confocal Imaging of Zebrafish Larvae

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All in vivo confocal imaging applied previously detailed methods13 ,57 (link). Briefly, larval zebrafish were treated with 200 nM PTU at 16 hpf to inhibit pigmentation. Prior to imaging, larvae were anesthetized with tricaine and embedded in 0.8 to 1.5% low-melt agarose. Images were collected on an Olympus FV1000 upright confocal microscope (20× or 60× water immersion objective, NA 0.95 and 1.10, respectively) using Olympus’ FV10-ASW software (v 4.1), except rho:YFP-NTR1.0 and 2.0 fish images which were collected on an Olympus FV3000 Fluoview inverted confocal microscope (30× silicone objective, NA 1.05) using Olympus’ FV31S-SWPAD software.
Sharrock A.V., Mulligan T.S., Hall K.R., Williams E.M., White D.T., Zhang L., Emmerich K., Matthews F., Nimmagadda S., Washington S., Le K.D., Meir-Levi D., Cox O.L., Saxena M.T., Calof A.L., Lopez-Burks M.E., Lander A.D., Ding D., Ji H., Ackerley D.F, & Mumm J.S. (2022). NTR 2.0: a rationally-engineered prodrug converting enzyme with substantially enhanced efficacy for targeted cell ablation. Nature methods, 19(2), 205-215.
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2

In vivo Confocal Imaging of Zebrafish Larvae

Check if the same lab product or an alternative is used in the 5 most similar protocols
All in vivo confocal imaging applied previously detailed methods13 ,57 (link). Briefly, larval zebrafish were treated with 200 nM PTU at 16 hpf to inhibit pigmentation. Prior to imaging, larvae were anesthetized with tricaine and embedded in 0.8 to 1.5% low-melt agarose. Images were collected on an Olympus FV1000 upright confocal microscope (20× or 60× water immersion objective, NA 0.95 and 1.10, respectively) using Olympus’ FV10-ASW software (v 4.1), except rho:YFP-NTR1.0 and 2.0 fish images which were collected on an Olympus FV3000 Fluoview inverted confocal microscope (30× silicone objective, NA 1.05) using Olympus’ FV31S-SWPAD software.
Sharrock A.V., Mulligan T.S., Hall K.R., Williams E.M., White D.T., Zhang L., Emmerich K., Matthews F., Nimmagadda S., Washington S., Le K.D., Meir-Levi D., Cox O.L., Saxena M.T., Calof A.L., Lopez-Burks M.E., Lander A.D., Ding D., Ji H., Ackerley D.F, & Mumm J.S. (2022). NTR 2.0: a rationally-engineered prodrug converting enzyme with substantially enhanced efficacy for targeted cell ablation. Nature methods, 19(2), 205-215.
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