Mouse anti bicd

Manufactured by Developmental Studies Hybridoma Bank

Mouse anti-BicD is a primary antibody that recognizes the BicD (Bicaudal-D) protein. BicD is a key regulator of microtubule-dependent intracellular transport processes. This antibody can be used to detect and study the localization and expression of BicD in various biological systems.

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Lab products found in correlation

Mouse anti dhc, by Developmental Studies Hybridoma Bank (3 mentions) Ab51603, by Abcam (2 mentions) Rabbit anti ctp, by Abcam (2 mentions) Gfp nanobody booster, by Proteintech (2 mentions) Mouse anti gfp, by Takara Bio (2 mentions) Rabbit anti gfp, by Proteintech (2 mentions) F1804, by Merck Group (2 mentions) Anti flag, by Merck Group (2 mentions) Rabbit anti gamma tubulin, by Merck Group (1 mentions) Rat anti vasa, by Developmental Studies Hybridoma Bank (1 mentions) Rabbit anti ha, by Cell Signaling Technology (1 mentions) Mouse anti gfp, by Abcam (1 mentions) Mouse anti ha, by Cell Signaling Technology (1 mentions) Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)

3 protocols using mouse anti bicd

Diverse Antibodies for Cellular Imaging

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The following primary antibodies were used: rabbit anti-Egl [31 (link)] (kindly provided by R. Lehmann); mouse anti-Dhc (Developmental Studies Hybridoma Bank; donor J. Scholey); mouse anti-BicD (Developmental Studies Hybridoma Bank, donor R. Steward); rat anti-Vasa (Developmental Studies Hybridoma Bank; donors A.C. Spradling/D/Williams); rabbit anti-gamma-tubulin (Sigma); mouse anti-GFP (Abcam); mouse anti-HA (Cell signaling technology); rabbit anti-HA (Cell signaling technology); rabbit anti-HOAP, guinea pig anti-HipHop and anti-Gag HeT-A (kindly provided by Y. Rong); rabbit anti-Gag HeT-A (kindly provided by E. Casacuberta (23) Alexa fluor conjugated secondary antibodies (Life Technologies) were diluted 1:500.

Kordyukova M., Morgunova V., Olovnikov I., Komarov P.A., Mironova A., Olenkina O.M, & Kalmykova A. (2018). Subcellular localization and Egl-mediated transport of telomeric retrotransposon HeT-A ribonucleoprotein particles in the Drosophila germline and early embryogenesis. PLoS ONE, 13(8), e0201787.

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Antibody Staining Protocol for Drosophila

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The following antibodies were used: anti-FLAG (Sigma Aldrich, F1804, 1:5000 for western, 1:500 for immunofluorescence), mouse anti-BicD (Clones 1B11 and 4C2, Developmental Studies Hybridoma Bank, 1:30 for immunofluorescence; 1:300 for western, donor R. Steward), rabbit anti-Ctp (Abcam, ab51603, 1:5000 for western), GFP nanobody booster (Chromotek; 1:500 for immunofluorescence), mouse anti-GFP (Clontech, JL-8, 1:5000 for western), rabbit anti-GFP (Chromotek; 1:3000 for immunofluorescence), C3G (generous gift from S. Hawley, mouse anti-C(3)G, 1:500), Orb (mouse anti-Orb, clone 4H8, 1:30 dilution), mouse anti-Dhc (Developmental Studies Hybridoma Bank, clone 2C11-2, 1:50; for immunofluorescence, donor J. Scholey), rabbit anti-Glued (generous gift from V. Gelfand, 1:500 for immunofluorescence). The following secondary antibodies were used: goat anti-rabbit Alexa 594, 555 and 488 (Life Technologies, 1:400, 1:400 and 1:200 respectively); goat anti-mouse Alexa 594, 555 and 488 (Life Technologies, 1:400, 1:400 and 1: 200 respectively) goat anti-mouse HRP (Pierce, 1:5000); and goat anti-rabbit HRP (Pierce, 1:5000).

Neiswender H., Goldman C.H., Veeranan-Karmegam R, & Gonsalvez G.B. (2021). Dynein light chain-dependent dimerization of Egalitarian is essential for maintaining oocyte fate in Drosophila. Developmental biology, 478, 76-88.

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Antibodies Used for Protein Detection

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The following antibodies were used: anti-FLAG (Sigma Aldrich, F1804, 1:5000 for western, 1:500 for immunofluorescence), mouse anti-BicD (Clones 1B11 and 4C2, Developmental Studies Hybridoma Bank, 1:30 for immunofluorescence; 1:300 for western, donor R. Steward), rabbit anti-Ctp (Abcam, ab51603, 1:5000 for western), GFP nanobody booster (Chromotek; 1:500 for immunofluorescence), mouse anti-GFP (Clontech, JL-8, 1:5000 for western), rabbit anti-GFP (Chromotek; 1:3000 for immunofluorescence), C3G (generous gift from S. Hawley, mouse anti-C(3)G, 1:500), Orb (mouse anti-Orb, clone 4H8, 1:30 dilution), mouse anti-Dhc (Developmental Studies Hybridoma Bank, clone 2C11-2, 1:50; for immunofluorescence, donor J. Scholey), rabbit anti-Glued (generous gift from V. Gelfand, 1:500 for immunofluorescence). The following secondary antibodies were used: goat anti-rabbit Alexa 594, 555 and 488 (Life Technologies, 1:400, 1:400 and 1:200 respectively); goat anti-mouse Alexa 594, 555 and 488 (Life Technologies, 1:400 , 1:400 and 1: 200 respectively) goat anti-mouse HRP (Pierce, 1:5000); and goat anti-rabbit HRP (Pierce, 1:5000).

Neiswender H., Goldman C.H., Veeranan‐Karmegam R., & Gonsalvez G.B. (2021). Dynein light chain-dependent dimerization of Egalitarian is essential for maintaining oocyte fate in Drosophila.

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