The hippocampus and prefrontal cortex of the mice were harvested 9 h after surgery. Anti-ZO-1 (1:500, Abcam, ab96587), anti-occludin (1:2,000, Abcam, ab167161), and anti-claudin-5 (1:500, Biorbyt, orb214680) antibodies were used to detect the expression of tight junction (TJ)-associated proteins in the hippocampus and prefrontal cortex. Anti-β-actin (1:10,000, TDY Biotech, ab37168) was used to normalize and control for loading differences in protein levels. The bands were measured using image analysis software (AlphaEaseFC software), and changes in protein levels were presented as folds of those in the control group.
Ab167161
Manufactured by Biorbyt
Ab167161 is a laboratory reagent supplied by Biorbyt. This product is a purified rabbit polyclonal antibody. The core function of this antibody is to specifically bind to and detect a target protein or antigen.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ab167161
1
Evaluating Tight Junction Proteins in Mouse Hippocampus and Prefrontal Cortex
2
Proteins Analysis of IEC-6 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total proteins from IEC-6 cells were extracted by RIPA lysis buffer (Beyotime Institute of Biotechnology) and the quantification of these proteins was conducted using BCA kits (Thermo Fisher Scientific, Inc.). Exposed to 10% SDS-PAGE, the proteins were then transferred onto PVDF membranes. After the procedure of impeding membranes with 5% nonfat milk and incubating with corresponding primary antibodies against ANGPT2 (Abcam, ab155106), barrier dysfunction-related proteins (ZO-1 (Abcam, ab221547), occludin (Abcam, ab167161), claudin-1 (Biorbyt, orb10447)), ERs-related proteins (GRP79 (Abcam, ab21685), CHOP (Biorbyt, orb314857), p-PERK (Biorbyt, orb474911), PERK (Biorbyt, orb338886)), Notch signaling-related proteins (Notch1 intracellular domain (NICD1) (Abcam, ab167441), HES1 (Abcam, ab108937)), and GAPDH (Abcam, ab9485) at 4°C overnight, secondary antibodies were applied to incubate membranes for 2 h. Finally, the protein bands were photographed using enhanced chemiluminescence (ECL).
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