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The LAMP1 antibody is a laboratory reagent used to detect the presence of the lysosome-associated membrane protein 1 (LAMP1) in cells. LAMP1 is a type I transmembrane glycoprotein that is primarily located in the lysosomal membrane and plays a role in the biogenesis and function of lysosomes. The LAMP1 antibody can be used in various experimental techniques, such as Western blotting, immunohistochemistry, and flow cytometry, to visualize and quantify the expression of LAMP1 in different cell types and tissues.

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Lab products found in correlation

Bleomycin, by Merck Group (1 mentions) Cytofix cytoperm and perm wash, by BD (1 mentions) Anti mouse cd45 magnetic beads, by Miltenyi Biotec (1 mentions) Hrp conjugated secondary antibody, by Santa Cruz Biotechnology (1 mentions) Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Collagenase a, by Roche (1 mentions) Macs cell separation column, by Miltenyi Biotec (1 mentions) Tcs spe confocal microscope, by Leica (1 mentions)

Close spelling variants of this product

Rat anti-LAMP1 antibody LAMP1 antibody (H4A3), LAMP1 primary antibody LAMP1

4 protocols using lamp1 antibody

1

Isolation and Analysis of Immune Cells

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Bleomycin was purchased from Millipore (Billerica, MA). Biotin conjugated rat anti-mouse CD16/32 and CD45 antibodies, PE- and Cy7-conjugated anti-mouse CD45 antibody, FITC-conjugated anti-mouse CD11b antibody and Cytofix/Cytoperm and Perm wash were from BD Biosciences (San Jose, CA). Anti-mouse CD45 magnetic beads and MACS Cell Separation columns were purchased from Miltenyl Biotec (San Diego, CA). Collagen I antibody was purchased from Rockland (Gilbertsville, PA). HRP-conjugated secondary antibodies are from Santa Cruz (Santa Cruz, CA). Collagenase A was purchased from Roche (Indianapolis, IN). Alexa Fluorconjugated secondary antibodies and fluorescein-conjugated type I collagen are from Life Technologies (Grand Island, NY). Rabbit IgG isotype control antibody was purchased from Southern Biotech (Birmingham, AL). Collagen III antibody was from Abcam (Cambridge, MA). LAMP1 antibody was from the University of Iowa Developmental Studies Hybridoma Bank. Adenoviruses expressing Cre was from the University of Iowa Gene Transfer Vector Core Facility. All other reagents were purchased from Sigma-Aldrich (St Louis, MO).
Kleaveland K.R., Velikoff M., Yang J., Agarwal M., Rippe R.A., Moore B.B, & Kim K.K. (2014). Fibrocytes Are Not an Essential Source of Type I Collagen During Lung Fibrosis. Journal of immunology (Baltimore, Md. : 1950), 193(10), 5229-5239.
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2

Immunostaining of Mouse Liver Tissues

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Fresh mouse liver tissues were fixed in 4% paraformaldehyde overnight and then switched to 20% sucrose and stored at 4 °C for approximately 24 h. Liver tissues then were embedded in O.C.T. and stored at −20 °C. Five-μm sections were used for immunostaining with indicated primary antibodies followed by Alexa 488 or Rhodamine 123-conjugated secondary antibody. The LAMP1 antibody was from Developmental Studies Hybridoma Bank (1D4B). Secondary antibodies were from Jackson ImmunoResearch. Confocal images were obtained using a Leica TCS SPE confocal microscope (DM550 Q).
Chao X., Niu M., Wang S., Ma X., Yang X., Sun H., Hu X., Wang H., Zhang L., Huang R., Xia M., Ballabio A., Jaeschke H., Ni H.M, & Ding W.X. (2023). High-throughput screening of novel TFEB agonists in protecting against acetaminophen-induced liver injury in mice. Acta Pharmaceutica Sinica. B, 14(1), 190-206.
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3

Lipid Trafficking in AML12 Cells

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AML12 cells were labeled with 7.5 µM BODIPY-(558/568)-C12 lipid for 2 h, washed with two exchanges of HBSS, and placed into regular culture media for 24 h with or without 50 µM lalistat. Following this 24 h “chase” period, cells were fixed and processed for immunofluorescence using a LAMP1 antibody (Cat. no. 1D4B, Developmental Studies Hybridoma Bank [DSHB], University of Iowa) to detect remaining fluorescent lipid in the lysosome.
Schulze R.J., Krueger E.W., Weller S.G., Johnson K.M., Casey C.A., Schott M.B, & McNiven M.A. (2020). Direct lysosome-based autophagy of lipid droplets in hepatocytes. Proceedings of the National Academy of Sciences of the United States of America, 117(51), 32443-32452.
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4

Evaluating ER Ca2+ Regulation Modulators

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Standard Western blotting protocols were used. HEK293T cells were treated every 4 hr for 24 hr with IP3R antagonists 2-APB and Xestospongin-C, ER Ca2+ chelator TPEN, and RyR antagonist DHBP. Lamp1 antibody was from Developmental Studies Hybridoma Bank (Iowa).
Garrity A.G., Wang W., Collier C.M., Levey S.A., Gao Q, & Xu H. (2016). The endoplasmic reticulum, not the pH gradient, drives calcium refilling of lysosomes. eLife, 5, e15887.
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