Fluorescent secondary antibodies (goat anti-rabbit, 10 μg/mL) were purchased from commercial vendors (see Supplementary Table 2 for list of fluorescent secondary antibodies). Retention (Fig. 1c ) was quantified via before-after proExM imaging mouse cortex as described below. Cortical sections of wild type (only used for Alexa 488 due to Thy1-YFP crosstalk) and Thy1-YFP brain slices (50 μm thick) were stained with anti-Homer primary antibody (Synaptic Systems; see Supplementary Table 4 ), and different secondary antibodies described in Supplementary Table 2 . Epifluorescence images of brain slices were taken with 4× 0.13 NA objective pre-gelation. Following proExM gelation and digestion, the brain slices were washed extensively with PBS (3 × 30 min), and epifluorescence images of the slice were taken again with identical imaging conditions. A region of interest in the cortex was used to determine the loss of fluorescence during proExM processing. Intensity measurements before and after sample processing were normalized by segmented area to account for fluorophore dilution.
Anti homer primary antibody
Manufactured by Synaptic Systems
The Anti-Homer primary antibody is a laboratory reagent used for the detection and analysis of the Homer protein in biological samples. It is a specific antibody that binds to the Homer protein, allowing researchers to study its expression and localization within cells or tissues.
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Lab products found in correlation
2 protocols using anti homer primary antibody
1
Quantifying Fluorescent Labeling Retention in proExM
2
Quantifying Fluorescent Labeling Retention in proExM
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Fluorescent secondary antibodies (goat anti-rabbit, 10 μg/mL) were purchased from commercial vendors (see Supplementary Table 2 for list of fluorescent secondary antibodies). Retention (Fig. 1c ) was quantified via before-after proExM imaging mouse cortex as described below. Cortical sections of wild type (only used for Alexa 488 due to Thy1-YFP crosstalk) and Thy1-YFP brain slices (50 μm thick) were stained with anti-Homer primary antibody (Synaptic Systems; see Supplementary Table 4 ), and different secondary antibodies described in Supplementary Table 2 . Epifluorescence images of brain slices were taken with 4× 0.13 NA objective pre-gelation. Following proExM gelation and digestion, the brain slices were washed extensively with PBS (3 × 30 min), and epifluorescence images of the slice were taken again with identical imaging conditions. A region of interest in the cortex was used to determine the loss of fluorescence during proExM processing. Intensity measurements before and after sample processing were normalized by segmented area to account for fluorophore dilution.
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