Cells were washed and stained with Fixable Viability Stain eFluor 780 (Thermo Fisher Scientific), anti-CD45 PerCPCY5.5, anti-GPR56 PE, anti-CD11b APC, anti-CD14 APC, anti-CD15 APC, anti-CD3 BV421 and anti-CD19 BV421 (all BD Biosciences, Le Pont de Claix) and processed on an Attune Next (Thermo Fischer Scientific) flow cytometer. Cell counts were obtained after manual gating on FlowJo V10.6.2 (Beckton Dickinson, Le Pont de Claix). Details are provided in the Supplementary Appendix.
Anti cd15 apc
Manufactured by BD
Anti-CD15 APC is a fluorescently-labeled antibody that binds to the CD15 cell surface marker. It is used for the identification and analysis of CD15-expressing cells in flow cytometry applications.
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Lab products found in correlation
Anti cd11b apc, by BD (1 mentions)
Anti cd14 apc, by BD (1 mentions)
Attune next, by Thermo Fisher Scientific (1 mentions)
Anti cd45 percp cy5, by BD (1 mentions)
Anti cd3 bv421, by BD (1 mentions)
Anti cd19 bv421, by BD (1 mentions)
Annexin 5 pe, by BioLegend (1 mentions)
Facsaria 2, by BD (1 mentions)
Anti cd14 pe, by BD (1 mentions)
Anti cd45 percp, by BD (1 mentions)
Easylyse, by Agilent Technologies (1 mentions)
3 protocols using anti cd15 apc
1
Flow Cytometric Immune Cell Profiling
2
Apoptosis Detection in GICs and GBM
3
Microparticle Binding Dynamics in SLE
Check if the same lab product or an alternative is used in the 5 most similar protocols
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MPs from 18 patients with SLE and 10 HCs were loaded with the fluorescent substrate 5 (and 6-)-carboxyfluorescein diacetate succinimidyl ester (CFSE) and incubated with autologous cells: leucocytes, purified from 100 µL whole blood subjected to erythrolysis with Easy Lyse (Dako; a 4.5% solution), or 100 µL washed whole blood, or 1 µL diluted (1:100) whole blood cells. Autologous serum (30% v/v) was present, and all sera were frozen within 60 min after collection. After incubation for 1, 5, 15, 30 and 60 min, respectively, 10 µL of the solution was transferred to 1 mL PBS on ice. The binding of MPs to granulocytes, monocytes and erythrocytes was measured by flow cytometry as the CFSE median fluorescence intensity (MFI) of each cell population.
Within morphological gates based on forward and side light scatter characteristics, and by staining with anti-CD45−PerCP, anti-CD15−APC and anti-CD14−PE (all from BD), granulocytes were identified as CD45+ and CD15+ cells; monocytes were identified as CD45+ and CD14+ cells; and erythrocytes were identified as CD45−, CD14− and CD15− cells.
Within morphological gates based on forward and side light scatter characteristics, and by staining with anti-CD45−PerCP, anti-CD15−APC and anti-CD14−PE (all from BD), granulocytes were identified as CD45+ and CD15+ cells; monocytes were identified as CD45+ and CD14+ cells; and erythrocytes were identified as CD45−, CD14− and CD15− cells.
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