Unless otherwise noted, all solvents and other reagents are commercially available and used without further purification. Purity and characterization of compounds were established by a combination of liquid chromatography-mass spectroscopy (LC-MS) and NMR analytical techniques and was >95% for all compounds. Silica gel column chromatography was carried out using prepacked silica cartridges from RediSep (ISCO Ltd.) and eluted using an Isco Companion system. Melting points were reordered on a MEL-TEMP® apparatus and are uncorrected. 1H- and 13C-NMR spectra were obtained on a Jeol 400 spectrometer at 400 MHz and 100 MHz, respectively. Chemical shifts are reported in δ (ppm) relative to residual solvent peaks or TMS as internal standards. Coupling constants are reported in Hz. High-resolution ESI-TOF mass spectra were acquired from the Mass Spectrometry Core at The Sanford-Burnham Medical Research Institute (Orlando, Florida). HPLC-MS analyses were performed on a Shimadzu 2010EV LCMS using the following conditions: Kromisil C18 column (reverse phase, 4.6 mm × 50 mm); a linear gradient from 10% acetonitrile and 90% water to 95% acetonitrile and 5% water over 4.5 min; flow rate of 1 mL/min; UV photodiode array detection from 200 to 300 nm. Continuous flow (microreactor) experiments were carried out using a Vapourtec R Series Flow Chemistry System.
Lcms 2010ev
Manufactured by Shimadzu
Sourced in Japan
The LCMS-2010EV is a liquid chromatography-mass spectrometry (LC-MS) system manufactured by Shimadzu. It is designed to perform qualitative and quantitative analysis of a wide range of compounds. The system combines a high-performance liquid chromatograph (HPLC) with a single quadrupole mass spectrometer, providing sensitive and reliable detection and identification of analytes.
Automatically generated - may contain errors
Lab products found in correlation
Am 500, by Bruker (5 mentions)
Euro ea 3000 chns analyzer, by Eurovector (5 mentions)
Supelco discovery c18 column, by Merck Group (4 mentions)
Perkin elmer 241 mc, by PerkinElmer (3 mentions)
70pf 254 plate, by Fujifilm (2 mentions)
Lcms it tof, by JEOL (2 mentions)
Gcmate 2, by JEOL (2 mentions)
Lc 20ad, by Shimadzu (2 mentions)
Uv 1800, by Shimadzu (2 mentions)
Drx 300, by Bruker (2 mentions)
Combiflash rf 200i, by Teledyne (2 mentions)
Drx 600, by Bruker (2 mentions)
Lc 20a system, by Shimadzu (2 mentions)
Companion system, by Teledyne (1 mentions)
400 spectrometer, by JEOL (1 mentions)
High performance liquid chromatography (hplc), by Beckman Coulter (1 mentions)
2400 2 analyzer, by PerkinElmer (1 mentions)
Am 300, by Bruker (1 mentions)
Lc 2010ht, by Shimadzu (1 mentions)
Lc 10ad, by Shimadzu (1 mentions)
53 protocols using lcms 2010ev
1
Characterization of Organic Compounds
2
Purification and Structural Elucidation of Compounds
3
Synthesis and Characterization of Anticancer Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
General: All reagents were purchased from Sigma-Aldrich Co. and used without further purification, except for the solvents used for flash chromatography and recrystallization. 1 H NMR spectra were recorded on a Bruker AM 300 at 300 MHz and chemical shifts are given in d referenced to the residual solvent peak. 13 C NMR spectra at 75.5 MHz on the same spectrometer, using chloroform-d (CDCl 3 ), dimethylsulfoxide-d 6 (DMSO-d 6 ). Melting points are uncorrected and were determined in open glass capillaries using a Mel-Temp II apparatus. A Shimadzu 2010-EV LC/MS was used for obtaining a LC/MS spectrum, using methanol or mixture of methanol-water (formic acid 0.1%) as solvents. All LC-MS spectras were obtained under isocratic elution in a reversed phase C 18 column. Microanalyses were performed on a Perkin-Elmer 2400-II Analyzer. Flash column chromatography was carried out on a Merck silica gel 60 plate (230-400 Mesh ASTM). Reaction progress was followed by thin-layer chromatography (Fluka Silica gel/TLC-cards). All solvents used for column chromatography and/or recrystallization were routinely distilled prior to use. Petroleum ether refers to the fraction with bp 40 o -60 o C. The known compounds 13 and 14 were synthesized as previously described [12, 13] for the purpose of evaluation of their antiproliferative activity.
4
Synthesis and Characterization of Peptides
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PMAP-37, PMAP-37(F34-R), and Chol-37(F34-R) were synthesized by Fmoc solid-phase peptide synthesis at Shanghai Apeptide Biological Technology Co., Ltd. (Shanghai, China). All peptides were purified by HPLC to high purity (≥95%), and the synthesized peptides were identified by MS. The HPLC and MS protocol are as follows. HPLC experiments of PMAP-37 and Chol-37(F34-R) were carried out on Waters 2690 and HPLC experiment of PMAP-37(F34-R) was carried out on SHIMADZU LC-2010HT. The instruments were equipped with a Waters SinoChrom ODS-BP column (5 μm, 4.6 × 250 mm) at a flow rate of 1.0 mL/min using a gradient of 5–65% acetonitrile in water with 0.1% TFA as the mobile phase. UV detection was performed at a wavelength of 220 nm. MS experiments of PMAP-37 and Chol-37(F34-R) were carried out on SHIMADZU LCMS-2010EV and MS experiment of PMAP-37(F34-R) was carried out on Agilent LCMS-6125B. The ESI source conditions were as follow. Nebulizer gas flow: 1.5 L/min; CDL temperature: 250 °C; capillary voltage: 1500 V; T. flow: 0.2 mL/min. The mass scan was in the range of m/z 400–2000.
5
Characterization of Alanylalanine by LCMS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Each sample in the previous section was diluted by water up to 10 µL. The final product, alanylalanine, was characterized by LCMS-2010EV electrospray mass spectrometry (Shimadzu, Kyoto, Japan) in positive-single ion monitoring (SIM) mode at m/z 199.20 using the analytical column Luna C8(2) 100 Å (150 mm × 4.6 mm, Phenomenex). The mobile phase consisted of 65% methanol. The flow rate was set at 0.2 mL/min and the injection volume was 8 µL. The column temperature was 60 °C. The peak area was calculated according to the protocol of the manufacturer (Shimadzu, Kyoto, Japan).
6
Spectroscopic Characterization of Triterpenoid Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The spectra were recorded at the Center for the Collective Use “Chemistry” of the Ufa Institute of Chemistry of the UFRC RAS and RCCU “Agidel” of the UFRC RAS. 1H and 13C NMR spectra were recorded on a “Bruker AM-500” (Bruker, Billerica, MA, USA, 500 and 125.5 MHz, respectively, δ, ppm, Hz) in CDCl3, internal standard tetramethylsilane. Mass spectra were obtained on a liquid chromatograph–mass spectrometer LCMS-2010 EV (Shimadzu, Kyoto, Japan). Melting points were detected on a micro table “Rapido PHMK05” (Nagema, Dresden, Germany). Optical rotations were measured on a polarimeter “PerkinElmer 241 MC” (PerkinElmer, Waltham, MA, USA) in a tube length of 1 dm. Elemental analysis was performed on a Euro EA-3000 CHNS analyzer (Eurovector, Milan, Italy); the main standard is acetanilide. Thin-layer chromatography analyses were performed on Sorbfil plates (Sorbpolimer, Krasnodar, Russian Federation), using the solvent system chloroform-ethyl acetate, 40:1. Substances were detected by 10% H2SO4 with subsequent heating to 100–120 °C for 2–3 min. Compounds 1 , 2 [57 (link)], 3 , 17–20 [24 ], 4 , 6 , 9 , 11 , 12 , 15 , 16 , 22, 23 [58 (link)], 5 [31 (link)], 7 [59 ], 8, 13 [22 (link)], 10 , 21 [30 ], 14 [60 (link)], 25 [61 (link)], 28 [62 (link)], and 35 [63 ] were prepared by the literature methods. Oleanolic acid 27 was purchased from Xian RongSheng Biotechnology Co., Ltd.
7
Phytochemical Analysis of P. friedrichstalianum
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The fractions obtained by partition of P. friedrichstalianum polar extract were analyzed in a liquid chromatograph LC-10AD Shimadzu (Kyoto, Japan) coupled to mass spectrometer Shimadzu LCMS-2010 EV (Kyoto, Japan). The injection of samples was performed in a loop injector Rheodyne 5 µL. A C18-column Shimadzu-5 µm (50 × 4.6 mm i.d.) was used. The mobile phase was acetonitrile–formic acid 0.1% (solvent A) and formic acid–H2O 0.1% (solvent B). The samples were eluted with a linear gradient which started with 5% to 60% of solvent A in 27 min; the solvent flow was set at 0.4 mL/min. The eluted compounds were monitored at two wavelengths λ 280 and 370 nm. The mass spectrometer was operated in positive and negative electrospray ionization (ESI) mode, 1.5 kV, CDL 300 °C, block 240 °C, gas flow (N2) to 1.5 L/min, CDL voltage 150 kV, Q array RF voltage 150 V, and detector voltage 1.5 kV. The positive and negative ions were detected between m/z 50 and 1000 u.
8
HPLC-ESI/MS Analysis of GSTeH
9
Comprehensive Characterization of Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
The reaction products were characterized by 1H and 13C NMR spectra that were recorded on spectrometers Bruker Avance 400 NMR (400.13 MHz and 100.62 MHz) and Bruker Ascend III HD 500 (500.17 MHz and 125.78 MHz), internal standard TMS, solvent DMSO-d6. The homo- and heteronuclear 2D experiments were performed by the standard pulse sequences of Bruker. IR spectra were recorded on a Bruker Vertex-70V FTIR and Specord M80 spectrometers. Electrospray ionization (ESI) mass spectra were obtained on a HPLC massspectrometer LCMS-2010EV (Shimadzu) in positive and negative ions mode at the corona discharge needle ionizing electrode and ionizing capillary potential of – 3.5 kV. Sample solution (direct syringe sample inlet) under ESI conditions was in methanol (acetonitrile), mobile phase was acetonitrile/water, 95/5. Mass-spectra was recorded on a device MALDI TOF Autoflex III firm Bruker (compounds2a-e ) with sinapinic acid as a matrix (see Supplementary data). Elemental analysis was performed on a Carlo Erba 1106 elemental analyzer. Melting points were determined on a Kofler hot-stage microscope and utilized uncorrected. Individuality and purity of synthesized compounds were controlled by means of TLC on Silufol UV-254 plates; I2 was used as developer.
+ Expand
The reaction products were characterized by 1H and 13C NMR spectra that were recorded on spectrometers Bruker Avance 400 NMR (400.13 MHz and 100.62 MHz) and Bruker Ascend III HD 500 (500.17 MHz and 125.78 MHz), internal standard TMS, solvent DMSO-d6. The homo- and heteronuclear 2D experiments were performed by the standard pulse sequences of Bruker. IR spectra were recorded on a Bruker Vertex-70V FTIR and Specord M80 spectrometers. Electrospray ionization (ESI) mass spectra were obtained on a HPLC massspectrometer LCMS-2010EV (Shimadzu) in positive and negative ions mode at the corona discharge needle ionizing electrode and ionizing capillary potential of – 3.5 kV. Sample solution (direct syringe sample inlet) under ESI conditions was in methanol (acetonitrile), mobile phase was acetonitrile/water, 95/5. Mass-spectra was recorded on a device MALDI TOF Autoflex III firm Bruker (compounds
10
Analytical Techniques for Compound Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Silica gel 70PF 254 Plate-Wako was used for TLC (thin layer chromatography) and column chromatography was performed on Wakogel®, 60 N, (particle size, 63–212 μm) or Wakogel®, C-300E, (particle size, 45–75 μm). Melting points were obtained on a Yanaco micro melting point apparatus and are uncorrected. Low-resolution mass spectra (LRMS) were recorded on Shimadzu LCMS-2010EV (ESI) and high-resolution mass spectra (HRMS) were recorded on Shimadzu LCMS-IT-TOF (ESI) or JEOL GC mate II (EI). 1H NMR spectra were recorded using Bruker AV 300 spectrometer, chemical shifts (δ) are quoted in parts per million (ppm) referenced to tetramethylsilane (0 ppm) or the residual solvent peak. 13C NMR spectra were recorded on the same spectrometer at 75 MHz, using the residual solvent peak as the internal reference. Optical rotations were recorded using a HORIBA SEPA-300 polarimeter.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!