Bicinchoninic acid bca protein estimation kit

All the chemicals and reagents used were of the highest purity available. Phenylmethanesulfonylfluoride, β–mercaptoethanesulfonic acid sodium salt, β–mercaptoethanol, Ethanolamine, Sepharose-4B, Freund's complete and incomplete adjuvant, Bicinchoninic acid (BCA) protein estimation kit, Tween-20 and FITC conjugated mouse anti-rabbit antibody were purchased from Sigma-Aldrich Chemicals (St Louis, MO) and used as received. Horseradish peroxidase-conjugated anti-rabbit IgG were purchased from Bangalore Genei (India) Pvt. Ltd. (Bangalore, India). Mouse monoclonal anti-Listeria LZH1 IgG1 and mouse monoclonal IgG2a specific for the human erythrocyte membrane protein (Protein4.2 (2G-12)) were procured from Santa Cruz Biotechnology, Inc., California and Abnova Corporation respectively.

All the chemicals and reagents used were of the highest purity available. α-LA, HRP, β-ME, DEAE-cellulose, Sepharose-4B, Sephacryl S-300, Sephacryl S-200, Freund’s complete and incomplete adjuvants, bicinchoninic acid (BCA) protein estimation kit and Tween-20 were purchased from Sigma-Aldrich Chemicals (St Louis, MO, USA) and used as received. Anti-HSA and anti-HRP goat IgG were purchased from MP Biomedicals, Mumbai, India. DTNB was purchased from Himedia, Mumbai, India. HRP-conjugated goat anti-rabbit IgG was purchased from Bangalore Genei Pvt. Ltd. (Bangalore, India), Protein concentrators (Amicon Ultra-15 centrifugal filter units) were purchased from Merck Millipore, India. Milk powder (Nestle Everyday, India), Baby feed (Nutricia Dexolac, India.) and raw bovine milk were purchased from the local grocery store. Whey was isolated from raw bovine milk by acid precipitation of casein. SDS and other reagents used were of analytical grade.