The antibodies used in the present study included anti-ZO-1 (Invitrogen, Thermo Fisher scientific), anti-Occludin-1 (Invitrogen, Thermo Fisher scientific), anti-Claudin-5 (Invitrogen, Thermo Fisher scientific), anti-Amyloid beta and anti-beta-actin (sc-47778) from Santa Cruz Biotechnology (Dallas, TX, USA).
Anti occludin 1
Manufactured by Thermo Fisher Scientific
Sourced in United States
Anti-Occludin-1 is a laboratory reagent used for research purposes. It is an antibody that specifically binds to the occludin protein, which is a component of tight junctions in cells. This product can be used in various experimental techniques, such as immunohistochemistry and Western blotting, to study the localization and expression of occludin in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti claudin 1, by Thermo Fisher Scientific (2 mentions)
Anti zo 1, by Santa Cruz Biotechnology (2 mentions)
Anti claudin 5, by Thermo Fisher Scientific (1 mentions)
Anti zo 1, by Thermo Fisher Scientific (1 mentions)
Goat anti mouse fitc, by Jackson ImmunoResearch (1 mentions)
Dextran sulfate sodium (dss), by MP Biomedicals (1 mentions)
Enzyme linked immunosorbent assay, by Cusabio (1 mentions)
Anti caspase 1 p10, by Santa Cruz Biotechnology (1 mentions)
Anti il 18, by Abcam (1 mentions)
Anti β actin, by Abcam (1 mentions)
Anti nlrp3 ag 20b 0014 c100, by Adipogen (1 mentions)
Anti asc, by Santa Cruz Biotechnology (1 mentions)
Anti il 1β, by Cell Signaling Technology (1 mentions)
3 protocols using anti occludin 1
1
Antibody Analysis of Tight Junctions
2
Immunofluorescence Analysis of Tight Junction Proteins
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Paraffin sections were heated in a water bath for 15 minutes for antigen extraction until dewaxing and hydration. Fragments were cultivated with anti-ZO-1 (#sc-33725; Santa Cruz Biotechnology, Dallas, TX, USA), anti-occludin-1 (#71-1500, Invitrogen, Carlsbad, CA, USA), and anti-claudin-1 (#51-9000, Invitrogen, USA) at 4 °C for a whole night, followed by fluorescein isothiocyanate (FITC) goat anti-mouse (1:500, #115-095-062, Jackson ImmunoResearch, West Grove, PA, USA) at 37 ℃ for 1 hour. The cells were soaked in phosphate-buffered saline (PBS) 3 times. For counterstaining, 50–100 µL of prepared 4’,6-diamidino-2-phenylindole (DAPI) dye solution (#KGA215, Nanjing, China) was added dropwise to each slide and incubated at 25 ℃ for 5 minutes in the dark. The slides were mounted with anti-extraction sealing tape and photographed by laser confocal microscopy. Semiquantitative analysis of ZO-1, claudin-1, and occludin-1 protein utterance was performed using the integrated optical density (IOD) and Image-Pro Plus 6.0 graphic analysis software (Media Cybernetics, Rockville, MD, USA).
3
Inflammatory Cytokine Profiling in DSS-Induced Colitis
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Dextran sodium sulfate (DSS) was obtained from MP Biomedicals (MW; 36000–50000, MP Biomedicals, USA). Absolute ethanol and xylene (Chemical Reagent, Sinopharm Group, China) were purchased. Enzyme-linked immunosorbent assay (ELISA) kits for IL-6 (#CSB-E04639m), IL-1β (#CSB-E08054m), IL-18 (#CSB-E04609m), IL-17 (#CSB-E04608m), TNF-α (#CSB-E04741m), and IL-10 (#CSBE04594m) were purchased from Cusabio Biotech Co. (Wuhan, Hubei, China). Anti-NLRP3 (#AG-20B-0014-C100, Adipogen, USA), Anti-ASC (#SC-22514R, Santa Cruz Biotechnology, USA), anticaspase 1 p10 (#SC-22166, Santa Cruz Biotechnology, USA), anti-IL-1β (#12242, Cell Signaling, USA), anti-IL-18 (#ab68435, Abcam, USA), anti-ZO-1 (#sc-33725; Santa Cruz Biotechnology, USA), antioccludin-1 (#71–1500, Invitrogen, USA), anticlaudin-1 (#51–9000, Invitrogen, USA), and anti-β-actin (#ab6276,Abcam, USA) antibodies were used. TRIzol reagent (Life Company, USA), DEPC water (Biyuntian Biotechnology, China), isopropanol (Chengdu Kelon Chemical, China), SYBR Premix Ex TaqTM, PrimeScriptTM RT master mix (#RR820A; Takara Bio, Inc), the hematoxylin-eosin staining kit (Cusabio Biotech, China), the DAB color reagent histochemistry kit (Cusabio Biotech, China), the BCA protein quantitative detection kit (#G2026, Cusabio Biotech, China), ECL (#G2014, Cusabio Biotech, China), and TBS buffer (#G0001-2L, Cusabio Biotech, China) were purchased.
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