Kaempferol

Manufactured by Chengdu Herbpurify

Sourced in China

Kaempferol is a naturally occurring flavonoid compound that can be extracted and purified for use in laboratory settings. It serves as a chemical standard and reference material for analytical and research purposes.

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Lab products found in correlation

3 protocols using kaempferol

Quantification of Flavonoids and Polyphenols

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Chromogenic substrate S-2238 was purchased from Chromogenix (Milan, Italy); bovine thrombin and argatroban were purchased from Sigma-Aldrich (St. Louis, MO, USA). The phosphate buffer saline (PBS) (pH 7.2-7.4, 0.01 M) and dimethyl sulfoxide (DMSO) were purchased from Solarbio (Beijing, China). Standard substance of galangin, kaempferol, astragaline, fisetin, quercetin, quercitrin, isoquercitrin, rutin, isorhamnetin, isorhamnetin-3-O-neohespeidoside, typhaneoside, myricetin, myricitrin, baicalein, baicalin, wogonin, wogonoside, hispidulin, scutellarein, genkwanin, hydroxygenkwanin, luteolin, luteoloside, 6-methoxyluteolin, dihydroquercetin, dihydromyricetin, naringenin, naringin, narirutin, hesperetin, hesperidin, neohesperidin, L-epicatechin, (-)-epigallocatechin, (-)-epicatechin gallate, proanthocyanidin B1 with 98% purity on the basis of HPLC analysis were obtained from Chengdu Herbpurify Co., Ltd. (Chengdu, China). Herbacetin and morin were obtained from Chengdu Must Bio-Technology Co., Ltd. (Chengdu, China). chrysin, apigenin, diosmetin and kaempferol-7-O-β-d-glucopyranoside were purchased from Chengdu Desite Bio-Technology Co., Ltd. (Chengdu, China).

Wang X., Yang Z., Su F., Li J., Boadi E.O., Chang Y.X, & Wang H. (2020). Study on Structure Activity Relationship of Natural Flavonoids against Thrombin by Molecular Docking Virtual Screening Combined with Activity Evaluation In Vitro. Molecules, 25(2), 422.

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Pneumolysin Cytotoxicity Evaluation

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Kaempferol (purity >98%) were obtained from Chengdu Herbpurify Co., Ltd. (China) and dissolved in dimethylsulfoxide (DMSO, Sigma–Aldrich). Fluorescent synthetic peptide substrate Dabcyl-QALPETGEE-Edans used in Sortase A activity inhibition assay was purchased from GL Biochem (Shanghai, China). A Cytotoxicity Detection Kit (Roche) and LIVE/DEAD™ Viability/Cytotoxicity Kit (Invitrogen) were applied to evaluate cell death caused by pneumolysin (PLY).

Xu L., Fang J., Ou D., Xu J., Deng X., Chi G., Feng H, & Wang J. (2022). Therapeutic potential of kaempferol on Streptococcus pneumoniae infection. Microbes and Infection, 25(3), 105058.

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Phytochemical Analysis of Medicinal Compounds

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QGC (provided by the laboratory of Affiliated Hospital of Southwest Medical University). Sodium Uric Acid Crystals (5 g, Rohn Reagent, Lot No. RH389360). Colchicine (100 mg/stem, Rohn Reagent, Lot No. RH341403). Maltol (Purity ≥98 %, Sichuan Weikeqi Biological Technology Co.,Ltd., Lot No. wkq22070114). Cafferic acid (Purity >98 %, Ltd，Lot No. DSTDE001301) and Catechin (Purity >99.87 %, Lot No. DSTDE000102) are from Chengdu Desite Biotechnology Co., Ltd. β-Sitosterol (Purity >98 %, Chengdu Ruifensidan Biotechnology Co., Ltd, Lot No. G00202005008), Ferulic acid (Lot No. RFS-A00211812016), Luteolin (Lot No. RFS-M00702004028), Baicalin (Lot No. RFS-H01811804026), Berberine (Lot No. RFS-Y03511811015), Quercetine (Lot No. RFS-H00911812016), Apigenin (Lot No. RFS-Q00211801029), Isorhamnetin (Lot No. RFS-Y03911811003) and Tangeritin (Lot No. RFS-J02202008018) all have purity >98 %, which were from Chengdu Herbpurify Co., Ltd. Kaempferol (Purity >98 %, Lot No. MSUT-13121112) and Neochlorogenic acid (Purity >99.40 %, Lot No. MSUT-21070910) were from Chengdu MUST Bio-Technology Co., Ltd. Mollugin (Purity >99.90 %, Chengdu MUST Bio-Technology Co., Ltd, Lot No. MSUT-21060211).

Xiang G., Yang L., Qin J., Wang S., Zhang Y, & Yang S. (2024). Revealing the potential bioactive components and mechanism of Qianhua Gout Capsules in the treatment of gouty arthritis through network pharmacology, molecular docking and pharmacodynamic study strategies. Heliyon, 10(10), e30983.

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