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G2 e mitter transponders

Manufactured by Starr Life Sciences

The G2 E-Mitter transponders are wireless devices designed for data collection and transmission in life science research applications. The core function of these transponders is to capture and transmit physiological data, such as temperature or other relevant parameters, from test subjects to a compatible data acquisition system.

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3 protocols using g2 e mitter transponders

1

Intraperitoneal E-Mitter Implantation in Mice

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G2 E-Mitter transponders (Starr Life Sciences, Oakmont, PA) were implanted intraperitoneally under isoflurane anesthesia (5% induction, 1.2% maintenance; Baxter Healthcare Corporation, Deerfield, IL) with Prevail (flunixin meglumine) analgesia (2.2 mg/kg sc at operation and daily for two days). Mice were studied at least one week after surgery. Tb was continuously measured by ER4000 energizer/receivers and 1 min means collected with VitalView software (Starr Life Sciences, Oakmont, PA), or by indirect calorimetry systems (Columbus Instruments, Columbus, OH)
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2

Intraperitoneal Transponder Implantation for Murine Physiological Monitoring

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G2 E-Mitter transponders (Starr Life Sciences, Oakmont, PA) were implanted intraperitoneally under anesthesia (isoflurane or ketamine 100 mg/kg ip and xylazine 10 mg/kg ip) with flunixin analgesia (2.2 mg/kg sc at operation and daily for two days). Mice were studied at least one week after surgery. Signals were acquired using ER4000 Energizer/Receivers and the manufacturer's software. Body weights are reported after subtraction of the weight of implanted E-Mitters. Body composition was measured by time domain Echo MRI 3-in-1 (Echo Medical Systems, Houston, TX) immediately after euthanasia and removal of the E-Mitter.
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3

Thermoregulation Monitoring in Otop1 Mice

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Littermate wild type and Otop1-/- mice were implanted intraperitoneally with G2 E-Mitter transponders (Starr Life Sciences, Oakmont, PA) and their Tb, total energy expenditure (TEE), respiratory exchange ratio (RER), and physical activity by beam break were measured using an Oxymax/CLAMS system (Columbus Instruments), as described [16 (link)]. The measurements were performed under different conditions, including ambient temperatures of 8 ˚C, 23 ˚C, 35 ˚C, fasting at 23 ˚C, and CL316243 (0.1 mg/kg, i.p.; ED50 is 0.004–0.008 mg/kg [17 (link)]) at 30 ˚C.
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