Silychristin and silydianin (≥ 98.0% purity) were purchased from ApexBio (Boston, MA, USA), silybin B, isosilybin A and isosilybin B (≥ 98.0% purity) were purchased from Sigma Aldrich (St. Louis, MO, USA), silybin A (50:50 mixture of silybin A and B) and d4-daidzein (≥ 99.0% purity) were purchased from Cayman Chemicals (Ann Arbor, MI, USA). HPLC-MS grade acetonitrile, methanol, and formic acid were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Ultrapure water was prepared using a Milli-Q water purification system (Millipore, MA, USA). All other reagents and solvents were reagent grade or better and were purchased from VWR (Visalia, CA, USA).
Muchiri R.N, & van Breemen R.B. (2020). Single Laboratory Validation of UHPLC-MS/MS Assays for Six Milk Thistle Flavonolignans in Human Serum. Journal of AOAC International, 104(1), 232-238.
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