5 5 dithiobis 2 nitrobenzoic acid dtnb

APAP was obtained from Adamas-beta (Shanghai, China). Reagents used for TrxR activity determination: 5,5’-Dithiobis-(2-nitrobenzoic acid) (DTNB) and nicotinamide adenine dinucleotide phosphate (NADPH) were from Biosharp (Anhui, China); Aurothioglucose (ATG) was purchased from Wako (Osaka, Japan). Glutathione reductase (GR) and glutathione (GSH) were purchased from Solarbio (Beijing, China). N-ethylmaleimide (NEM) was purchased from Sigma-Aldrich (Saint Louise, MO, USA). Anti-GSH antibody (from mice) was purchased from Abcam (Cambridge, UK), anti-thioredoxin1 (Trx1), and anti-Trx2 antibodies were from IMCO (Stockholm, Sweden); all other antibodies used in the study were obtained from Proteintech (Hubei, China). Sep-Pak C18 cartridge was purchased from Waters Corporation (Milford, MA, USA).

Cisplatin (DDP) was purchased from Aladdin (Shanghai, China). NADPH and 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB) were obtained from Biosharp (Guangzhou, China). Aurothioglucose (ATG) was purchased from WAKO (Tokyo, Japan). Glutathione reductase (GR) and iodoacetamide (IAM) were purchased from Sigma-Aldrich (St. Louis, MO, USA). RIPA lysis buffer was obtained from Beyotime Biotechnology (Shanghai, China). Selenium-deficient (SD, 0.01 mg/kg), selenium-normal (SN, 0.21 mg/kg), and selenium-enriched (SE, 1.01 mg/kg) diets were manufactured by Medicience (Nanning, China). All other reagents were obtained from the Sigma-Aldrich Chemical Co. (St. Louis, MO, USA) or Fisher Scientific Ltd. (Waltham, MA, USA). The pure recombinant wild-type rat TrxR1 protein, and anti-Trx1 and anti-TrxR1 antibodies were obtained from IMCO Corporation, Stockholm, Sweden (www.imcocorp.se accessed on 1 January 2020). Other antibodies were obtained from Proteintech (Wuhan, China). C57BL/6j mice were provided by Ensiweier (Chongqing, China).