54 rotavapor r 220

Ultrapure water obtained from Mili-Q ® purification system. Silica gel adsorbent 60 (230-400 mesh) for chromatographic column was obtained from Merck KGaA, German. The solvents used in the preparation of extracts were all analytical grade obtained from Vetec. Thin Layer Chromatography (TLC), silica gel 60 chromate plates with UV254 fluorescence indicator, 0.20 mm thick (MACHEREY -NAGEL-MN) and Amberlite® XAD16N 20-60 mesh (Sigma-Aldrich) were used. The rotary evaporator (54-Rotavapor R-220, with vacuum pump Vacuum Contoller V-805, brand: Büchi and water circulator) was used to concentrate the samples. The leaves (1.0 kg) from I. edulis were dried at room temperature, ground and extracted with EtOH using ultrasonic bath for 20 minutes. The ethanolic extract was subjected to a liquidliquid partition with organic solvents, providing four fractions: hexane fraction, ethyl acetate fraction, n-butanol fraction and hydromethanolic fraction.

The plant material from M. elliptica was collected at the Experimental Station of the Mogi-Guaçu Biological Reserve (São Paulo state, Brazil) and the plant material from A. glandulosa was collected at Ipueiras (Tocantins, Brazil). The exsiccates were deposited at the Herbarium in Tocantins (HTO) of the Federal University of Tocantins in Porto National city, under number 3341.
The plant material was dried at room temperature, and subjected to grinding using a knife mill (Willye -NL-226-02). The obtained powder was extracted by maceration using hexane and methanol. The methanolic extract was concentrated on a rotary evaporator (54-Rotavapor R-220, brand: Büchi, with vacuum pump Vacuum Contoller V-805, brand: Büchi and water circulator), yielding a crude, highly viscous extract which was diluted in a methanol: water mixture (1:9 v/v) and subjected to the liquidliquid partition with ethyl acetate and n-butanol.
The drug celecoxib has been commercially available. In Brazil, only Pfizer (Celebra®) markets this drug. In addition, the drug doxorubicin has been commercially available. In Brazil, this drug is market by Libbs (Fauldoxo®) and by Pfizer (Adriblastine®). The solvents used in the preparation of extracts and fractions were all analytical grade obtained from Vetec.