In total, 1 × 106 cells were plated in 6‐well plates and treated with 20 ng/mL EGF, 10 μmol/L SB203580, 10 μmol/L 2‐DG alone or in combinations for 24 h, and the cell culture supernatants and cells were collected separately. Next, the lactate concentration in the culture supernatant was determined using the Lactate detection kit (#A019‐2‐1, Nanjing Jiancheng Bioengineering, Nanjing, Jiangsu, China) according to the manufacturer's protocol. Harvested cells were stained with trypan blue, and viable cells were counted using an automated cell counter (Bio‐Rad, Hercules, CA, USA). Then, the adjusted lactate concentration was calculated according to the following formula: adjusted lactate concentration = lactate concentration in experimental group / the ratio of cell number in the experimental group to that in the control group.
Lactate detection kit
Manufactured by Nanjing Jiancheng
Sourced in China
The Lactate Detection Kit is a laboratory instrument designed to measure the concentration of lactate in biological samples. It provides a quantitative analysis of lactate levels, which is a key indicator of various metabolic processes and physiological conditions.
Automatically generated - may contain errors
Lab products found in correlation
Automated cell counter, by Bio-Rad (1 mentions)
Anti hif 1α antibody, by Abcam (1 mentions)
Dcfh da, by Meilun (1 mentions)
Lactate dehydrogenase assay kit, by Nanjing Jiancheng (1 mentions)
Bilirubin, by Tokyo Chemical Industry (1 mentions)
Anti cd31 antibody, by Wuhan Servicebio Technology (1 mentions)
Chlorin e6, by J&K Scientific (1 mentions)
N n diisopropylcarbodiimide, by Tokyo Chemical Industry (1 mentions)
Anti ldhb, by Proteintech (1 mentions)
Anti c myc antibody, by Proteintech (1 mentions)
Cou 6, by Merck Group (1 mentions)
Anti ldha, by Wuhan Servicebio Technology (1 mentions)
Glucose detection kit, by Merck Group (1 mentions)
Glucose detection kit, by Nanjing Jiancheng (1 mentions)
Pk activity detection kit, by Solarbio (1 mentions)
Microplate reader, by Thermo Fisher Scientific (1 mentions)
Multiskan go microplate spectrophotometer, by Thermo Fisher Scientific (1 mentions)
9 protocols using lactate detection kit
1
Lactate production in EGF-treated cells
2
Chlorin e6-Mediated Photodynamic Therapy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chlorin e6 was purchased from J&K Scientific Ltd. (Beijing, China). Diclofenac, morpholin-4-yl-acetic acid hydrochloride, 1-(3-dimethylaminopropyl)-3 ethylcarbodiimide hydrochloride (EDC), 4-dimethylaminopyridine (DMAP) and trimethylamine (TEA) were purchased from Aladdin (Shanghai, China). Bilirubin and N,N′-diisopropylcarbodiimide (DIC) were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). mPEG2k-NH2 and Fmoc-NH-PEG2k-NH2 were purchased from Ponsure Biotechnology (Shanghai, China). Cou-6 was obtained from Sigma–Aldrich (USA). DiD and DCFH-DA were obtained from Meilunbio (Dalian, China). The annexin V-fluoresceine isothiocyanate (FITC) apoptosis detection kit and calcium-AM were obtained from Yeason (Shanghai, China). Lactate detection kit and lactate dehydrogenase assay kit were obtained from Nanjing Jiancheng Bioengineering Institute (Naijing, China). Anti-HIF-1α antibody, Cy3 goat anti-rabbit IgG (H + L) and Alexa fluor 647 goat anti-rabbit IgG (H + L) secondary antibody were purchased from Abcam (Hongkong, China). Anti-LDHA and anti-CD31 antibody were purchased from Servicebio Company (Wuhan, China). Anti-LDHB and anti-c-MYC antibody were purchased from Proteintech (Wuhan, China). Anti-VEGFα and anti-angiopoietin-2 antibody were purchased from Abclonal Company (Wuhan, China).
3
Metabolic Profiling of G-Rh2 Treatment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were plated at a density of 4.0 × 105 cells/well in a six-well plate, incubated at 37℃ for 24 h to allow adherence, and then treated with different concentrations of G-Rh2 for 24 h. Subsequently, the cells were cultured in DMEM without FBS for serum starvation. The samples were assayed using a glucose detection kit (Sigma), lactate detection kit (Nanjing Jiancheng Bio), and pyruvate content detection kit (Solarbio, China). After adding the detection reagent, the optical density values were measured at 540 nm, 530 nm, and 520 nm using a microplate reader. A standard curve was generated by analyzing different standard dilutions, and the resulting OD values were utilized to determine glucose intake, lactification, and pyruvic acid production.
4
Glucose and Lactate Measurement in Chondrocytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chondrocytes were seeded into 24-well plates at a cell density of 5 × 10 4 /mL and incubated for 24 h. As instructed by the manufacturer's protocol, glucose consumption and lactate production were measured by using Glucose Detection Kit (Jiancheng Biotechnology, Nanjing, China) and Lactate Detection Kit (Jiancheng Biotechnology) respectively.
5
Quantification of Pyruvate and Lactate
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The concentrations of pyruvate and lactate were measured using pyruvate kinase (PK) activity detection kit (Solarbio, China), lactate detection kit (Nanjing jiancheng, China) and glucose consumption (Nanjing jiancheng, China) according to manufacturer’s instructions.
6
Lactate Production in Hepatocarcinoma Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The effect of CRKL overexpression on lactate production of hepatocarcinoma cells was measured using a lactate detection kit (Nanjing Jiancheng Bioengineering Institute). Each group 1 × 104 cells in 200 μL 10% FBS supplemented with DMEM from each group were seeded into a separate well of 96‐well plate, incubated at 37°C with 5% CO2 for 6, 12, 18 and 24 hours, removed into a 0.6 mL Eppendorf tube and centrifugated with 560 g for 10 minutes. Then, 2 μL of supernatant from each group was mixed well with 100 μL enzyme working liquid and 20 μL chromogenic reagent in a 96‐well pate, and incubated at 37°C for 10 minutes. Then, 200 μL stop reagent was added to each well for absorbance assay at 530 nm using a microplate reader (Thermo). Meanwhile, 2 μL of calibrator (3 mmol/L) was used for calibration. Results were the averages from triplicate measurements.
7
Ketamine Effect on Lactate Production
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HA1800 cells were seeded in 10-cm dishes and maintained for 24 h, after which they were treated with ketamine and/or an ERK1/2 inhibitor for 6 h. Thereafter, the culture medium from each sample was transferred to an Eppendorf (EP) tube, and lactate production was then measured by a lactate detection kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturer’s protocol.
8
Quantifying Chondrocyte Lactate Secretion
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Lactate secretion levels were detected using a Lactate Detection kit (Jiancheng Biotechnology, Nanjing, China). Chondrocyte culture medium (100 μL) was collected at 24, 48, 72, and 96 h after co-culture, and added to each well of a 96-well plate. After 10 min of incubation with the working solution and chromogenic agent at 37°C, 100 μL stop solution was added into each well. The absorbance levels were detected at a wavelength of 530 nm with a Multiskan GO microplate spectrophotometer (Thermo Fisher Scientific, Waltham, USA).
9
Sperm Lactate Content Measurement
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sperm lactate content was measured with a lactate detection kit (Nanjing Jiancheng, China) according to the manufacturer's instruction. Briefly, the level of lactate was measured by detecting the NADH formed consequently to lactate oxidation by LDH with a microplate reader at 340 nm (27 (link)).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!