Mouse monoclonal anti cd8 antibody clone c8 144b

Manufactured by Agilent Technologies

Sourced in Denmark

The mouse monoclonal anti-CD8 antibody (clone C8/144B) is a laboratory reagent designed for use in immunological applications. The antibody recognizes the CD8 cell surface glycoprotein, which is expressed on a subset of T cells and natural killer cells. This product is intended for research use only and its specific applications should be evaluated by the user.

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Lab products found in correlation

Envision system, by Agilent Technologies (1 mentions) Autostainer plus, by Agilent Technologies (1 mentions) Bond polymer refine red detection kit, by Leica camera (1 mentions) Aperio at system, by Leica Biosystems (1 mentions)

2 protocols using mouse monoclonal anti cd8 antibody clone c8 144b

Immunohistochemical Markers in Gallbladder Cancer

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IHC for CD1a, S100 and CD8 was performed using a single representative block of formalin-fixed, paraffin-embedded GBC tissue specimen obtained from each patient. Sections (4 μm) were deparaffinized, and antigen retrieval was performed using Histofine^Ⓡ Heat Processor Solution pH 9 (Nichirei Biosciences, Tokyo, Japan) and an automatically controlled thermostat (Histofine^Ⓡ HEAT PROII; Nichirei Biosciences). The following primary antibodies were used: mouse monoclonal anti-CD1a antibody (clone 010; IS06930–2; prediluted; Dako, Glostrup, Denmark), mouse monoclonal anti-CD8 antibody (clone C8/144B; GA62361–2; prediluted; Dako) and rabbit polyclonal anti-S100 antibody (GA50461–2 J; prediluted; Dako). The Envision+^Ⓡ System (Dako) was used as the secondary antibody. Slides were visualized using diaminobenzidine tetrahydrochloride, and nuclei were counterstained with hematoxylin. An Autostainer Plus^Ⓡ (Dako) was used for staining of specimens.

Kai K., Tanaka T., Ide T., Kawaguchi A., Noshiro H, & Aishima S. (2020). Immunohistochemical analysis of the aggregation of CD1a-positive dendritic cells in resected specimens and its association with surgical outcomes for patients with gallbladder cancer. Translational Oncology, 14(1), 100923.

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Quantifying Immune Cell Infiltration in Melanoma

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Single-marker IHC was performed on formalin-fixed paraffin-embedded (FFPE) tumor sample sections, (4 μm thickness) after heat-induced antigen retrieval (HIER, citrate buffer, pH 6.0), obtained from patients with melanoma at the designated treatment timepoints. Granzyme B was localized by immuno-labeling tissue sections with mouse mAb (clone GrB-7; DAKO) at 1:25 dilution followed by detection with the EnVisionTM G2 System/AP kit and visualization by Permanent Red Chromogen substrate staining. CD8 was labeled in FFPE tissue sections using mouse monoclonal anti-CD8 antibody (clone C8/144B; DAKO) at a 1:100 dilution followed by detection with the BOND Polymer Refine Red Detection Kit (Leica) and AP Red chromogen substrate visualization. Whole-slide scans were imaged using the Aperio-AT system (Leica Biosystems) and transferred into the HALO (Indica Labs) platform for quantitative digital image analysis.

Andtbacka R.H., Wang Y., Pierce R.H., Campbell J.S., Yushak M., Milhem M., Ross M., Niland K., Arbeit R.D., Parasuraman S., Bickley K., Yeung C.C., Aicher L.D., Smythe K.S, & Gan L. (2022). Mavorixafor, an Orally Bioavailable CXCR4 Antagonist, Increases Immune Cell Infiltration and Inflammatory Status of Tumor Microenvironment in Patients with Melanoma. Cancer Research Communications, 2(8), 904-913.

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