Log In Sign up
The largest database of trusted experimental protocols

Hla dr apc fire 750

Manufactured by BioLegend
Visit Supplier

HLA-DR-APC/Fire™ 750 is a fluorescently-labeled antibody product used for the detection and analysis of HLA-DR expression on cell populations. It is designed for flow cytometry applications.

Automatically generated - may contain errors

Lab products found in correlation

Cd4 percp cy5, by BioLegend (4 mentions) Cd25 pe, by BioLegend (4 mentions) Cd3 fitc, by BioLegend (4 mentions) Cd8 brilliant violet 510, by BioLegend (4 mentions) Cd38 brilliant violet 421, by BioLegend (4 mentions) Cobas amplicor, by Roche (4 mentions) Cd57 allophycocyanin apc, by BioLegend (3 mentions) Facscanto 2, by BD (2 mentions) Cytometer setup and tracking beads, by BD (1 mentions) Cd25 pe cy7, by BD (1 mentions) Cd19 apc h7, by BD (1 mentions) Cd3 buv805, by BD (1 mentions) Cd8 bv650, by BD (1 mentions) Cd16 bv650, by BD (1 mentions) Cd33 bv510, by BD (1 mentions) Cd45 af700, by BD (1 mentions) Mertk bv421, by BioLegend (1 mentions) Compbead, by BD (1 mentions) Cd206 pe cy7, by BioLegend (1 mentions) Cd14 percp cy5, by BD (1 mentions)

6 protocols using hla dr apc fire 750

1

Multiparametric Flow Cytometry of tPCLS

Check if the same lab product or an alternative is used in the 5 most similar protocols
Single‐cell suspensions of tPCLS were generated as described above. Single‐cell suspensions were blocked with FcR blocking reagent (Miltenyi Biotec) in 0.5% PBS‐BSA for 20 min, stained with fluorochrome‐conjugated antibodies, and analyzed on a FACSSymphony A5SE flow cytometer (BD Biosciences). Live single cells were identified by FSC and SSC characteristics. The data were analyzed using FlowJo V10 (TreeStar). All antibodies and secondary reagents were titrated to determine optimal concentrations. Comp‐Beads (BD) were used for single‐color compensation to create multicolor compensation matrices. For gating, fluorescence minus one control was used. The instrument calibration was controlled daily using Cytometer Setup and Tracking Beads (BD Biosciences). The following antibodies were used: CD3‐BUV805 (#612896, BD Biosciences), CD4‐BB630 (#562316, BD Biosciences), CD8‐BV650 (#743067, BD Biosciences), CD14‐PerCP‐Cy5.5 (#561116, BD Biosciences), CD15‐BUV805 (#742057, BD Biosciences), CD16‐BV650 (#563692, BD Biosciences), CD19‐APC‐H7 (#560252, BD Biosciences), CD25‐PE‐Cy7 (#557741, BD Biosciences), CD33‐BV510 (#563257, BD Biosciences), CD45‐AF700 (#368514, BD Biosciences), CD80‐BV711 (#740801, BD Biosciences), CD206‐PE/Cy7 (#321124, BioLegend), CD326‐FITC (#324203, BioLegend), HLA‐DR‐APC/Fire750 (#307658, BioLegend), MerTK‐BV421 (#367603, BioLegend).
Karger A., Mansouri S., Leisegang M.S., Weigert A., Günther S., Kuenne C., Wittig I., Zukunft S., Klatt S., Aliraj B., Klotz L.V., Winter H., Mahavadi P., Fleming I., Ruppert C., Witte B., Alkoudmani I., Gattenlöhner S., Grimminger F., Seeger W., Pullamsetti S.S, & Savai R. (2023). ADPGK‐AS1 long noncoding RNA switches macrophage metabolic and phenotypic state to promote lung cancer growth. The EMBO Journal, 42(18), e111620.
+ Open protocol
+ Expand
2

Quantifying Immune Activation Markers in HIV

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sera samples of immunological responders and non-responders were collected to measure the bacterial translocation markers. Following the standard protocols, human Lipopolysaccharides (LPS) ELISA Kit (CUSABIO; Wuhan, China) and Human soluble CD14 (sCD14) ELISA Kit (MultiSciences, Hangzhou, China) were used to test plasma LPS and sCD14. Flow cytometry and Cobas Amplicor (Roche Molecular Systems Inc., Branchburg, New Jersey, United States) were used to quantify CD4 + /CD8 + T-cells and HIV-1 RNA, respectively. Fresh anticoagulated whole blood was used to quantify the expressing markers of immune activation (CD25 +, CD38 +, HLADR +, or CD38 + /HLA-DR +) of CD4 + and CD8 + T-cells and immune senescence (CD57 +) by BD FACS Canto II flow cytometer (BD Biosciences, California, United States). The antibodies needed during the experiment were purchased from Biolegend (San Diego, CA), including CD3-FITC, CD4- PerCP/Cy5.5, CD8-Brilliant Violet 510™, CD38-Brilliant Violet 421, CD25-PE, HLA-DR-APC/Fire™ 750, and CD57-allophycocyanin (APC).
Xie Y., Sun J., Hu C., Ruan B, & Zhu B. (2021). Oral Microbiota Is Associated With Immune Recovery in Human Immunodeficiency Virus-Infected Individuals. Frontiers in Microbiology, 12, 794746.
+ Open protocol
+ Expand
3

Quantifying Immune Cell Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols
To quantify the level of activation, proliferation and cell death, cryopreserved CD4 subsets remaining from the sort were thawed. Approximately 2 million cells were stimulated with either DMSO (Sigma Aldrich, Cat no D2650-5X10ML) or PMA/ionomycin (final concentration of 10nM PMA and 0.5μM ionomycin) for 72 h. After the stimulation, 1.5 million cells were washed and surface stained for live/dead aqua, CD69 Alexa Fluor 647 (Biolegend, Cat no 310918, clone FN50), HLA-DR APC-Fire750 (Biolegend, Cat no 307658, clone L243), and CD38 V450 (BD biosciences, Cat no 561378, clone HIT2). Cells were then permeabilized using BD Cytofix/Cytoperm buffer (BD Biosciences, Cat no 554714) and stained intracellularly with Ki-67 PE (BD biosciences, Cat no 556027, clone B56). Samples were run within 2 h of staining completion on BD LSR Fortessa flow cytometer and the data was analyzed using FlowJo version 10.
Rasmussen T.A., Zerbato J.M., Rhodes A., Tumpach C., Dantanarayana A., McMahon J.H., Lau J.S., Chang J.J., Gubser C., Brown W., Hoh R., Krone M., Pascoe R., Chiu C.Y., Bramhall M., Lee H.J., Haque A., Fromentin R., Chomont N., Milush J., Van der Sluis R.M., Palmer S., Deeks S.G., Cameron P.U., Evans V, & Lewin S.R. (2022). Memory CD4+ T cells that co-express PD1 and CTLA4 have reduced response to activating stimuli facilitating HIV latency. Cell Reports Medicine, 3(10), 100766.
+ Open protocol
+ Expand
4

Quantification of T-cell Markers in HIV

Check if the same lab product or an alternative is used in the 5 most similar protocols
Quantifications of CD4+ and CD8+ T-cells as well as HIV-1 RNA were carried out in HIV-infected individuals using flow cytometry and Cobas Amplicor (Roche Molecular Systems Inc., Branchburg, New Jersey, USA) as the clinical routine. The percentage of CD4+ and CD8+ T cells expressing markers of activation (CD25+, CD38+, HLADR+, or CD38+/HLA-DR+) and senescence (CD57+) were quantified by the BD FACS Canto II flow cytometer (BD Biosciences, California, USA) using fresh anticoagulated whole blood. Antibody such as CD3-FITC, CD4- PerCP/Cy5.5, CD8-Brilliant Violet 510™, CD38-Brilliant Violet 421, CD25-PE, HLA-DR-APC/Fire™ 750, and CD57-allophycocyanin (APC) were purchased from Biolegend (San Diego, CA).
Xie Y., Sun J., Wei L., Jiang H., Hu C., Yang J., Huang Y., Ruan B, & Zhu B. (2021). Altered gut microbiota correlate with different immune responses to HAART in HIV-infected individuals. BMC Microbiology, 21, 11.
+ Open protocol
+ Expand
5

Immunophenotyping of HIV Infection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Quanti cations of and CD8+ as well as HIV-1 RNA were carried out in HIV-infected individuals using ow cytometry and Cobas Amplicor (Roche Molecular Systems Inc., Branchburg, New Jersey, USA) as the clinical routine. The percentage of CD4+ and CD8+ T cells expressing markers of activation (CD25+, CD38+, HLADR+, or CD38+/HLA-DR+) and senescence (CD57+) were quanti ed by the BD FACS Canto II ow cytometer (BD Biosciences, California, USA) using fresh anticoagulated whole blood. Antibody such as CD3-FITC, CD4-PerCP/Cy5.5, CD8-Brilliant Violet 510™, CD38-Brilliant Violet 421, CD25-PE, HLA-DR-APC/Fire™ 750, and CD57-allophycocyanin (APC) were purchased from Biolegend (San Diego, CA).
Xie Y., Sun J., Li W., Jiang H., Hu C., Yang J., Huang Y., Ruan B., & Zhu B. (2020). Altered gut microbiota correlate with different immune response to HAART in HIV-infected individuals.
+ Open protocol
+ Expand
6

Quantification of T-cell Subsets in HIV

Check if the same lab product or an alternative is used in the 5 most similar protocols
Quanti cations of CD4+ and CD8+ T-cells as well as HIV-1 RNA were carried out in HIV-infected individuals using ow cytometry and Cobas Amplicor (Roche Molecular Systems Inc., Branchburg, New Jersey, USA) as the clinical routine. The percentage of CD4+ and CD8+ T cells expressing markers of activation (CD25+, CD38+, HLADR+, or CD38+/HLA-DR+) and senescence (CD57+) were quanti ed by the BD FACS Canto II ow cytometer (BD Biosciences, California, USA) using fresh anticoagulated whole blood. Antibody such as CD3-FITC, CD4-PerCP/Cy5.5, CD8-Brilliant Violet 510™, CD38-Brilliant Violet 421, CD25-PE, HLA-DR-APC/Fire™ 750, and CD57-allophycocyanin (APC) were purchased from Biolegend (San Diego, CA).
Xie Y., Sun J., Li W., Jiang H., Hu C., Yang J., Huang Y., Ruan B., & Zhu B. (2020). Altered gut microbiota correlate with different immune response to HAART in HIV-infected individuals.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!