Pureyield plasmid miniprep
The PureYield™ Plasmid Miniprep is a lab equipment product designed for the purification of plasmid DNA from bacterial cultures. It provides a simple and efficient method for isolating high-quality plasmid DNA for various downstream applications.
Lab products found in correlation
9 protocols using pureyield plasmid miniprep
Cloning and Sequencing of Bisulfite-Treated DNA
Recombinant Peptide Expression in B. subtilis
Restriction enzymes, T 4 DNA ligase and PureYield Plasmid Miniprep were from Promega (Madison, WI, USA). Enterokinase was from Sigma (St. Louis, MO, USA). DL-2000 DNA markers were from Takara Biotechnology (Dalian, China). DNA Purification Kit, Protein Markers were from Transgen Biotechnology (Bejing, China). Mouse anti-His monoclonal antibody and goat anti-mouse IgG-AP antibody were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Ni-NTA-agarose beads were from Qiagen (Hilden, Germany).
DNA Amplification and Cloning Protocol
Illumina-based Sequencing of Vibrio cholerae S24
All primers used in this study are shown in Table
TcC Variant Mutagenesis and Expression
For HiC the codon-optimized genes (i.e. E. coli codon usage) of wild-type, I167Q and L64H/I167Q variants were purchased from GeneArt® (Life Technologies, USA). The genes were cloned into the vector pET26b(+) and chemically transformed by heat shock into E. coli XL-10.
Amplification and Sequencing of Mycoplasma obg Gene
Plasmid Preparation and Enzymatic Manipulation
Chickpea PGIPs Cloning and Sequencing
Preparation of Lipid Biosynthesis Enzymes
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